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Flow Cytometry Instrument Setting as a Crucial Checkpoint for Optimal T-Cell Analysis and Sorting

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Cytotoxic T-Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2325))

Abstract

Although flow cytometry and cell sorting are widely used by immunologists both for basic and translation research, many aspects of these techniques should be optimized to obtain reproducible and meaningful data. In this chapter we provide some protocols and tips on instrument setting, multicolor panel design and T-cell immunophenotyping and proliferation assay.

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Author information

Authors and Affiliations

  1. Flow Cytometry Core, Humanitas Clinical and Research Center, Milan, Italy

    Achille Anselmo & Federico Simone Colombo

Authors
  1. Achille Anselmo
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  2. Federico Simone Colombo
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Corresponding author

Correspondence to Achille Anselmo .

Editor information

Editors and Affiliations

  1. Department of Surgical and Medical Sciences, University of Foggia, Foggia, Italy

    Margherita Gigante

  2. Department of Surgical and Medical Sciences, University of Foggia, Foggia, Italy

    Elena Ranieri

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Anselmo, A., Colombo, F.S. (2021). Flow Cytometry Instrument Setting as a Crucial Checkpoint for Optimal T-Cell Analysis and Sorting. In: Gigante, M., Ranieri, E. (eds) Cytotoxic T-Cells. Methods in Molecular Biology, vol 2325. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1507-2_1

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  • DOI: https://doi.org/10.1007/978-1-0716-1507-2_1

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1506-5

  • Online ISBN: 978-1-0716-1507-2

  • eBook Packages: Springer Protocols

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