Abstract
Implantation of bacteria embedded in a fibrin clot allows for successful establishment of sepsis in preclinical models. This model allows the investigator to modulate the strain of bacteria as well as the bacterial load delivered. As it allows for a slow release of standardized bacteria, the use of a fibrin clot model may be considered in studying the initial and later phases of sepsis and the host response to infection. Here we describe methods for performing the fibrin clot model of sepsis.
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Acknowledgments
This work was supported by the National Institutes of Health grants K08GM126313 (to Dr. Ghanta) and R01GM118456 (to Dr. Perrella).
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Ghanta, S., Kwon, MY., Perrella, M.A. (2021). Induction of Sepsis Via Fibrin Clot Implantation. In: Walker, W.E. (eds) Sepsis. Methods in Molecular Biology, vol 2321. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1488-4_3
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DOI: https://doi.org/10.1007/978-1-0716-1488-4_3
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