Skip to main content
Springer Nature Link
Log in

Plant Lipids

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Dorothea Bartels

    1. Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Bonn, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Peter Dörmann

    1. IMBIO Institut, Universität Bonn, Bonn, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2295)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

About this book

This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments and organelles; lipid signaling, lipid-protein interactions, and imaging; and lipid databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Plant Lipids: Methods and Protocols is a valuable guide for experienced researchers and undergraduate, graduate, and Ph.D. students. This book is also an excellent resource for novice scientists with little to no experience inlipid experiments who are interested in approaching this field experimentally.

 


Similar content being viewed by others

Keywords

Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Basic Methods of Lipid Isolation and Analysis

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Lipid Isolation from Plants

      • Jesse D. Bengtsson, James G. Wallis, John Browse
      Pages 3-13

    3. Three Methods to Extract Membrane Glycerolipids : Comparing Sensitivity to Lipase Degradation and Yield

      • Samira Mahboub, Zachery D. Shomo, R. Maxwell Regester, Mahaa Albusharif, Rebecca L. Roston
      Pages 15-27

    4. Thin-Layer Chromatography

      • Georg Hölzl, Peter Dörmann
      Pages 29-41

    5. Lipid Analysis by Gas Chromatography and Gas Chromatography–Mass Spectrometry

      • Mathias Brands, Philipp Gutbrod, Peter Dörmann
      Pages 43-57

    6. 14C-Tracing of Lipid Metabolism

      • Hari Kiran Kotapati, Philip D. Bates
      Pages 59-80

    7. Methods of Lipid Analyses for Microalgae : Charophytes, Eustigmatophytes, and Euglenophytes

      • Masako Iwai, Shiori Shibata, Hiroyuki Ohta, Koichiro Awai
      Pages 81-97

  3. Mass Spectrometry and NMR Analysis

    1. Front Matter

      Pages 99-99
      Download chapter PDF

    2. Direct Infusion Mass Spectrometry for Complex Lipid Analysis

      • Katharina Gutbrod, Helga Peisker, Peter Dörmann
      Pages 101-115

    3. Fatty Acid Composition by Total Acyl Lipid Collision-Induced Dissociation Time-of-Flight (TAL-CID-TOF) Mass Spectrometry

      • Pamela Tamura, Carl Fruehan, David K. Johnson, Paul Hinkes, Todd D. Williams, Ruth Welti
      Pages 117-133

    4. Targeted Analysis of the Plant Lipidome by UPLC-NanoESI-MS/MS

      • Cornelia Herrfurth, Yi-Tse Liu, Ivo Feussner
      Pages 135-155

    5. Mass Spectrometry -Based Profiling of Plant Sphingolipids from Typical and Aberrant Metabolism

      • Rebecca E. Cahoon, Ariadna Gonzalez Solis, Jennifer E. Markham, Edgar B. Cahoon
      Pages 157-177

    6. Analysis of Free and Esterified Sterol Content and Composition in Seeds Using GC and ESI-MS/MS

      • Richard Broughton, Frédéric Beaudoin
      Pages 179-201

    7. Techniques for the Measurement of Molecular Species of Acyl-CoA in Plants and Microalgae

      • Richard P. Haslam, Tony R. Larson
      Pages 203-218

    8. Quantification of Acyl-Acyl Carrier Proteins for Fatty Acid Synthesis Using LC-MS/MS

      • Lauren M. Jenkins, Jeong-Won Nam, Bradley S. Evans, Doug K. Allen
      Pages 219-247

    9. Structural Analysis of Glycosylglycerolipids Using NMR Spectroscopy

      • Wiebke Knaack, Georg Hölzl, Nicolas Gisch
      Pages 249-272

  4. Lipid Isolation and Analysis from Plant Tissues Cell Compartments and Organelles

    1. Front Matter

      Pages 273-273
      Download chapter PDF

    2. Analysis of Extracellular Cell Wall Lipids: Wax , Cutin , and Suberin in Leaves, Roots, Fruits, and Seeds

      • Johanna Baales, Viktoria V. Zeisler-Diehl, Lukas Schreiber
      Pages 275-293

    3. Isolation of Lipid Droplets for Protein and Lipid Analysis

      • Patrick J. Horn, Kent D. Chapman, Till Ischebeck
      Pages 295-320
Back to top

Editors and Affiliations

  • Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Bonn, Germany

    Dorothea Bartels

  • IMBIO Institut, Universität Bonn, Bonn, Germany

    Peter Dörmann

Bibliographic Information

  • Book Title: Plant Lipids

  • Book Subtitle: Methods and Protocols

  • Editors: Dorothea Bartels, Peter Dörmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1362-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1361-0Published: 29 May 2021

  • Softcover ISBN: 978-1-0716-1364-1Published: 30 May 2022

  • eBook ISBN: 978-1-0716-1362-7Published: 28 May 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 472

  • Number of Illustrations: 30 b/w illustrations, 52 illustrations in colour

  • Topics: Plant Sciences, Lipidology

Publish with us

Policies and ethics

Back to top