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Mechanical Permeabilization as a New Method for Assessment of Mitochondrial Function in Insect Tissues

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Mitochondrial Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2276))

Abstract

Respirometry analysis is an effective technique to assess mitochondrial physiology. Insects are valuable biochemical models to understand metabolism and human diseases. Insect flight muscle and brain have been extensively used to explore mitochondrial function due to dissection feasibility and the low sample effort to allow oxygen consumption measurements. However, adequate plasma membrane permeabilization is required for substrates/modulators to reach mitochondria. Here, we describe a new method for study of mitochondrial physiology in insect tissues based on mechanical permeabilization as a fast and reliable method that do not require the use of detergents for chemical permeabilization of plasma membrane, while preserves mitochondrial integrity.

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Acknowledgments

We would like to thank Mrs. Geane C. Braz for the excellent technical assistance on maintenance of A. aegypti colony. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001, by the Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq) [grant numbers 404153/2016-0 MFO, and 483334/2013-8 AG], and the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) [grant numbers E-26/102.333/2013, E-26/203.043/2016, and E-26/111.169/2011]. AG and MFO are CNPq fellows [grant numbers 402409/2012-4 and 303044/2017-9] and from PAPD-FAPERJ [grant number E-44/208702/2014]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Federal University of Rio de Janeiro, Institute of Medical Biochemistry Leopoldo de Meis, Rio De Janeiro, RJ, Brazil

    Alessandro Gaviraghi, Yan Aveiro, Stephanie S. Carvalho, Rodiesley S. Rosa, Matheus P. Oliveira & Marcus F. Oliveira

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  1. Alessandro Gaviraghi
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  2. Yan Aveiro
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  3. Stephanie S. Carvalho
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  4. Rodiesley S. Rosa
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  5. Matheus P. Oliveira
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  6. Marcus F. Oliveira
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Editors and Affiliations

  1. Department of Pharmaceutical Sciences, Midwestern University, Glendale, AZ, USA

    Volkmar Weissig

  2. Cochin Hospital, Cochin Institute, INSERM U1016, PARIS, France

    Marvin Edeas

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Gaviraghi, A., Aveiro, Y., Carvalho, S.S., Rosa, R.S., Oliveira, M.P., Oliveira, M.F. (2021). Mechanical Permeabilization as a New Method for Assessment of Mitochondrial Function in Insect Tissues. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine . Methods in Molecular Biology, vol 2276. Springer, New York, NY. https://doi.org/10.1007/978-1-0716-1266-8_5

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  • DOI: https://doi.org/10.1007/978-1-0716-1266-8_5

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