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Mitochondrial Coenzyme Q10 Determination Via Isotope Dilution Liquid Chromatography –Tandem Mass Spectrometry

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Mitochondrial Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2275))

Abstract

Coenzyme Q10 (CoQ10) is an essential part of the mitochondrial respiratory chain . Here, we describe an accurate and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for determination of mitochondrial CoQ10 in isolated mitochondria . In the assay, mitochondrial suspensions are spiked with CoQ10-[2H9] internal standard (IS), extracted with organic solvents and CoQ10 quantified by LC-MS/MS using multiple reaction monitoring (MRM).

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Author information

Authors and Affiliations

  1. Centre for Inherited Metabolic Diseases, Karolinska University Hospital, Stockholm, Sweden

    Erik Hedman

  2. Laboratory division HUSLAB, Helsinki University Central Hospital, Helsinki, Finland

    Outi Itkonen

Authors
  1. Erik Hedman
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  2. Outi Itkonen
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Correspondence to Erik Hedman .

Editor information

Editors and Affiliations

  1. Department of Pharmaceutical Sciences, Midwestern University, College of Pharmacy, Glendale, AZ, USA

    Volkmar Weissig

  2. Cochin Hospital, Cochin Institute, INSERM U1016, Paris, France

    Marvin Edeas

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Hedman, E., Itkonen, O. (2021). Mitochondrial Coenzyme Q10 Determination Via Isotope Dilution Liquid Chromatography –Tandem Mass Spectrometry. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine . Methods in Molecular Biology, vol 2275. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1262-0_21

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  • DOI: https://doi.org/10.1007/978-1-0716-1262-0_21

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1261-3

  • Online ISBN: 978-1-0716-1262-0

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