Abstract
Extracellular vesicles (EVs) are vesicles released by cells, which due to their cargo and cell membrane proteins induce changes in the recipient cells. These vesicles can be a novel option to induce stem cell differentiation. Here we described a method to induce mesenchymal stem cell differentiation (MSC) into neuron-like cells using small EVs from neurons. First, we will describe a method based on neurons to induce adipocyte derived stem cells differentiation, a type of MSC, by coculturing both using inserts. Secondly, we will describe a follow-up method by using only isolated neuron-derived small EVs to directly induce ADSC differentiation in neuron-like cells. Importantly, in both methods it is possible to avoid the direct cell-to-cell contact, thus allowing for the study of soluble factors role during stem cell differentiation.
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Acknowledgments
We are grateful for the funding supporting the research involving extracellular vesicles from the University of Sao Paulo. Additional supported by São Paulo Research Foundation (FAPESP) grants: #2015/17897-9, #2014/22887-0, #2015/21829-9, #2017/21266-0 and #2013/08135-2, the National Council for Scientific and Technological Development—CNPq grant number #420152/2018-0 and CAPES—Finance Code 001. The Authors are observer members of the COST Action CA16119 In vitro 3-D total cell guidance and fitness (CellFit).
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Roballo, K.C.S., Ambrosio, C.E., da Silveira, J.C. (2021). Protocol to Study the Role of Extracellular Vesicles During Induced Stem Cell Differentiation. In: Brevini, T.A., Fazeli, A., Turksen, K. (eds) Next Generation Culture Platforms for Reliable In Vitro Models . Methods in Molecular Biology, vol 2273. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1246-0_3
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DOI: https://doi.org/10.1007/978-1-0716-1246-0_3
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