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Ex Vivo Model of Spontaneous Neuroretinal Degeneration for Evaluating Stem Cells’ Paracrine Properties

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In Vitro Models for Stem Cell Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2269))

Abstract

Ex vivo neuroretina cultures closely resemble in vivo conditions, retaining the complex neuroretina cells dynamics, connections, and functionality, under controlled conditions. Therefore, these models have allowed advancing in the knowledge of retinal physiology and pathobiology over the years. Furthermore, the ex vivo neuroretina models represent an adequate tool for evaluating stem cell therapies over neuroretinal degeneration processes.

Here, we describe a physically separated co-culture of neuroretina explants with stem cells to evaluate the effect of stem cells paracrine properties on spontaneous neuroretinal degeneration.

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Author information

Authors and Affiliations

  1. Instituto Universitario de Oftalmobiología Aplicada (IOBA), Universidad de Valladolid, Campus Miguel Delibes, Paseo de Belén 17, Valladolid, Spain

    Ivan Fernandez-Bueno & Ricardo Usategui-Martin

  2. Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León, Valladolid, Spain

    Ivan Fernandez-Bueno

  3. Red Temática de Investigación Cooperativa en Salud (RETICS), Oftared, Instituto de Salud Carlos III, Valladolid, Spain

    Ivan Fernandez-Bueno

Authors
  1. Ivan Fernandez-Bueno
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  2. Ricardo Usategui-Martin
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Corresponding author

Correspondence to Ivan Fernandez-Bueno .

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Editors and Affiliations

  1. Department of Visceral, Transplant, Thoracic and Vascular Surgery, Applied Molecular Hepatology Lab, University of Leipzig Medical Center, Leipzig, Germany

    Peggy Stock

  2. Department of Visceral, Transplant, Thoracic and Vascular Surgery, Applied Molecular Hepatology Lab, University of Leipzig Medical Center, Leipzig, Germany

    Bruno Christ

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Fernandez-Bueno, I., Usategui-Martin, R. (2021). Ex Vivo Model of Spontaneous Neuroretinal Degeneration for Evaluating Stem Cells’ Paracrine Properties. In: Stock, P., Christ, B. (eds) In Vitro Models for Stem Cell Therapy. Methods in Molecular Biology, vol 2269. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1225-5_9

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  • DOI: https://doi.org/10.1007/978-1-0716-1225-5_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1224-8

  • Online ISBN: 978-1-0716-1225-5

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