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Protein-Ligand Interactions

Methods and Applications

  • Book
  • © 2021
  • Latest edition

Overview

Editors:
  1. Tina Daviter

    1. The Institute of Cancer Research, London, UK

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  2. Christopher M. Johnson

    1. MRC Laboratory of Molecular Biology, Cambridge, UK

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  3. Stephen H. McLaughlin

    1. MRC Laboratory of Molecular Biology, Cambridge, UK

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  4. Mark A. Williams

    1. ISMB BiophysX Centre, Birkbeck, University of London, London, UK

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2263)

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About this book

This third edition provides new and updated chapters detailing a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins with their natural ligands, drugs or other binding partners. Chapters detail natural substrates, potential drug leads, quantitative understanding of the mechanism of interaction, and different techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Third Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions.


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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Overview

    1. Front Matter

      Pages 1-1
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    2. Assessing and Improving Protein Sample Quality

      • Bertrand Raynal, Sébastien Brûlé, Stephan Uebel, Stefan H. Knauer
      Pages 3-46

    3. A Familiar Protein–Ligand Interaction Revisited with Multiple Methods

      • Xiaochun Li-Blatter, Ludovit Zweifel, Timothy Sharpe
      Pages 47-79

  4. Screening for Ligand Binding

    1. Front Matter

      Pages 199-199
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    2. Indirect Detection of Ligand Binding by Thermal Melt Analysis

      • Joseph Shaw, Christopher Stubbs
      Pages 201-215

    3. The Use of Acoustic Mist Ionization Mass Spectrometry for High-Throughput Screening

      • Helen Plant, David Murray, Hannah Semple, Gareth Davies, Ian Sinclair, Geoffrey A. Holdgate
      Pages 217-230

    4. Ligand Discovery: High-Throughput Binding: Fluorescence Polarization ( Anisotropy )

      • Geoffrey A. Holdgate, Paul E. Hemsley
      Pages 231-246

    5. Fragment Screening by NMR

      • Ben J. Davis
      Pages 247-270

  5. Nucleotide Binding and Hydrolysis

    1. Front Matter

      Pages 271-271
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    2. A Quick Primer in Fluorescence-Based Equilibrium and Pre-steady State Methods for Determining Protein–Nucleotide Affinities

      • Harland E. Brandon, Hans-Joachim Wieden
      Pages 273-287

    3. Measurement of Nucleotide Hydrolysis Using Fluorescent Biosensors for Phosphate

      • Simone Kunzelmann
      Pages 289-318

  6. Binding Nucleic Acids

    1. Front Matter

      Pages 319-319
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    2. Gel-Based Analysis of Protein–Nucleic Acid Interactions

      • James A. W. Stowell, Terence T. L. Tang, Maximilian Seidel, Lori A. Passmore
      Pages 321-339
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Editors and Affiliations

  • The Institute of Cancer Research, London, UK

    Tina Daviter

  • MRC Laboratory of Molecular Biology, Cambridge, UK

    Christopher M. Johnson, Stephen H. McLaughlin

  • ISMB BiophysX Centre, Birkbeck, University of London, London, UK

    Mark A. Williams

Bibliographic Information

  • Book Title: Protein-Ligand Interactions

  • Book Subtitle: Methods and Applications

  • Editors: Tina Daviter, Christopher M. Johnson, Stephen H. McLaughlin, Mark A. Williams

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1197-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1196-8Published: 21 April 2021

  • Softcover ISBN: 978-1-0716-1199-9Published: 21 April 2021

  • eBook ISBN: 978-1-0716-1197-5Published: 20 April 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 492

  • Number of Illustrations: 43 b/w illustrations, 80 illustrations in colour

  • Topics: Life Sciences, general, Chemistry/Food Science, general, Pharmacology/Toxicology

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