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Chemical Synthesis of PDZ Domains

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PDZ Mediated Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2256))

Abstract

Developments in chemical protein synthesis have enabled the generation of tailor-made proteins including incorporation of many types of modifications into proteins, enhancing our ability to control site-specificity of protein posttranslational modifications (PTMs), modify protein backbones and introduce photocrosslinking probes. For PDZ (postsynaptic density protein, disks large, zonula occludens) protein domains, expressed protein ligation (EPL) has been employed to introduce analogs of cognate amino acids, amide-to-ester bond mutations, and phosphorylations in the study of PDZ domain-mediated protein-protein interactions (PPIs). Here, we present protocols for EPL of PDZ domains focusing on phosphorylation and amide-to-ester modifications in the PDZ domain proteins.

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Author information

Authors and Affiliations

  1. Department of Drug Design and Pharmacology, Center for Biopharmaceuticals, University of Copenhagen, Copenhagen, Denmark

    Christin Kossmann, Sana Ma, Louise S. Clemmensen & Kristian Strømgaard

Authors
  1. Christin Kossmann
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  2. Sana Ma
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  3. Louise S. Clemmensen
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  4. Kristian Strømgaard
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Corresponding author

Correspondence to Kristian Strømgaard .

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Editors and Affiliations

  1. Centre de Recherche en CancÕrologie de Marseille, Aix-Marseille University, Inserm, CNRS, Institut Paoli-Calmettes, Marseille, France

    Jean-Paul Borg

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Kossmann, C., Ma, S., Clemmensen, L.S., Strømgaard, K. (2021). Chemical Synthesis of PDZ Domains. In: Borg, JP. (eds) PDZ Mediated Interactions. Methods in Molecular Biology, vol 2256. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1166-1_12

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  • DOI: https://doi.org/10.1007/978-1-0716-1166-1_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1165-4

  • Online ISBN: 978-1-0716-1166-1

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