Abstract
Vegetative insecticidal protein in Bacillus can be detected through the process of PCR (Polymerase Chain Reaction). PCR approach is based on the amplification of the gene and will assign the amplified gene into the Vip gene family but this approach will not identify the specific gene of the family. Therefore, PCR-RFLP technique is used to detect the type of the Vip genes within a same family.
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Reference
Hernández-RodrÃguez CS, Boets A, Van Rie J, Ferré J (2009) Screening and identification of vip genes in Bacillus thuringiensis strains. J Appl Microbiol 107:219–225
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Senthilkumar, M., Amaresan, N., Sankaranarayanan, A. (2021). Detection of Vegetative Insecticidal Protein (Vip) in Bacillus. In: Plant-Microbe Interactions. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1080-0_71
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DOI: https://doi.org/10.1007/978-1-0716-1080-0_71
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1079-4
Online ISBN: 978-1-0716-1080-0
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