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Antibody Arrays pp 247–256Cite as

Immune Cell Isolation from Murine Intestine for Antibody Array Analysis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2237))

Abstract

Gut mucosal immune cells play an essential role in health due to their ability to orchestrate host signaling events in response to exogenous antigens. These antigens may originate from microorganisms including viruses, commensal or pathogenic bacteria, or single-celled eukaryotes, as well as from dietary foodstuff-derived proteins or products. A critical technological capacity to understand host responses to antigens is the ability to efficiently isolate and functionally characterize immune cells from intestinal tissues. Additionally, after characterization, it is of paramount importance to understand the exact functions of these immune cells under different disease states or genetic variables. Here, we outline methods for immune cell isolation from murine small and large intestines with the goal of undertaking a functional analysis of isolated cell types using antibody array platforms.

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Abbreviations

IEL :

Intraepithelial lymphocytes

LI:

Large intestine

LPL:

Lamina propria lymphocytes

PP:

Peyer’s patch

SI:

Small intestine

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Acknowledgments

R.M.J. is supported, in part, by NIH Grant R01DK098391.

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Authors and Affiliations

  1. Division of Pediatric Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA

    Joshua A. Owens & Rheinallt M. Jones

Authors
  1. Joshua A. Owens
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  2. Rheinallt M. Jones
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Corresponding author

Correspondence to Joshua A. Owens .

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Editors and Affiliations

  1. RayBiotech, Peachtree Corners, GA, USA

    Kelly C. Whittaker

  2. RayBiotech Life, Peachtree Corners, GA, USA

    Ruo-Pan Huang

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Owens, J.A., Jones, R.M. (2021). Immune Cell Isolation from Murine Intestine for Antibody Array Analysis. In: Whittaker, K.C., Huang, RP. (eds) Antibody Arrays. Methods in Molecular Biology, vol 2237. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1064-0_21

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  • DOI: https://doi.org/10.1007/978-1-0716-1064-0_21

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1063-3

  • Online ISBN: 978-1-0716-1064-0

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