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Experimental Mouse Models of Ragweed- and Papain-Induced Allergic Conjunctivitis

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Animal Models of Allergic Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2223))

Abstract

Mouse models of allergic conjunctivitis mimic various aspects of human allergic conjunctivitis. They are useful as acute models of allergic conjunctivitis to study immunological aspects of this condition. In this chapter, we will describe ragweed-pollen-induced experimental allergic conjunctivitis (mostly driven by adaptive immunity), and papain-soaked contact lens-induced experimental allergic conjunctivitis (mostly driven by innate immunity). Giemsa staining of histological sections is used for quantification of the number of infiltrating eosinophils, which is useful to evaluate the severity of the allergic inflammation. Immunohistochemical staining and quantitative PCR are used to clarify spatiotemporal expression of proinflammatory molecules in the conjunctival tissue. Flow cytometric analysis of conjunctival tissue is used for the detection of innate lymphoid cell type 2 (ILC2) in the ocular surface tissues.

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References

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Authors and Affiliations

  1. Laboratory of Ocular Atopic Diseases, Department of Ophthalmology, Juntendo University Graduate School of Medicine, Tokyo, Japan

    Akira Matsuda, Toshiaki Hirakata & Yosuke Asada

  2. Laboratory of System Biology, Institute of Medical Science, The University of Tokyo, Tokyo, Japan

    Yosuke Asada & Susumu Nakae

Authors
  1. Akira Matsuda
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  2. Toshiaki Hirakata
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  3. Yosuke Asada
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  4. Susumu Nakae
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Corresponding author

Correspondence to Akira Matsuda .

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Editors and Affiliations

  1. Department of Biomedical Sciences, University of North Dakota School of Medicine & Health Sciences, Grand Forks, ND, USA

    Kumi Nagamoto-Combs

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Matsuda, A., Hirakata, T., Asada, Y., Nakae, S. (2021). Experimental Mouse Models of Ragweed- and Papain-Induced Allergic Conjunctivitis. In: Nagamoto-Combs, K. (eds) Animal Models of Allergic Disease. Methods in Molecular Biology, vol 2223. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1001-5_10

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  • DOI: https://doi.org/10.1007/978-1-0716-1001-5_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1000-8

  • Online ISBN: 978-1-0716-1001-5

  • eBook Packages: Springer Protocols

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