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Identification of Endothelial Cells and Their Progenitors

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Vascular Morphogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2206))

Abstract

Blood vessel formation is a key feature in physiologic and pathologic processes. Once considered a homogeneous cell population that functions as a passive physical barrier between blood and tissue, endothelial cells (ECs) are now recognized to be quite “heterogeneous.” While numerous attempts to enhance endothelial repair and replacement have been attempted using so called “endothelial progenitor cells” it is now clear that a better understanding of the origin, location, and activation of stem and progenitor cells of the resident vascular endothelium is required before attempting exogenous cell therapy approaches. This chapter provides an overview for performance of single-cell clonogenic studies of human umbilical cord blood circulating endothelial colony-forming cells (ECFC) that represent distinct precursors for the endothelial lineage with vessel forming potential.

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Author information

Authors and Affiliations

  1. Division of Pediatric Rheumatology, The Hospital for Sick Children, Toronto, ON, Canada

    Ellen Go

  2. Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine, Indianapolis, IN, USA

    Mervin C. Yoder

Authors
  1. Ellen Go
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  2. Mervin C. Yoder
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Correspondence to Mervin C. Yoder .

Editor information

Editors and Affiliations

  1. Department of Basic Medical Sciences, Neurosciences and Sensory Organs, University of Bari Medical School, Bari, Italy

    Domenico Ribatti

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Go, E., Yoder, M.C. (2021). Identification of Endothelial Cells and Their Progenitors. In: Ribatti, D. (eds) Vascular Morphogenesis. Methods in Molecular Biology, vol 2206. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0916-3_3

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  • DOI: https://doi.org/10.1007/978-1-0716-0916-3_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0915-6

  • Online ISBN: 978-1-0716-0916-3

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