Abstract
Salmonella enterica is a Gram-negative intracellular pathogen that causes a range of life-threatening diseases in humans and animals worldwide. In a systemic infection, the ability of Salmonella to survive/replicate in macrophages, particularly in the liver and spleen, is crucial for virulence. Transformed macrophage cell lines and primary macrophages prepared from mouse bone marrow are commonly used models for the study of Salmonella infection. However, these models raise technical or ethical issues that highlight the need for alternative methods. This chapter describes a technique for immortalizing early hematopoietic progenitor cells derived from wild-type or transgenic mice and using them to produce macrophages. It validates, through a specific example, the interest of this cellular approach for the study of Salmonella infection.
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Acknowledgments
Z.F. was supported by the China Scholarship Council (CSC Grant). The plasmid for conditional expression of MSCV-ERHBD-Hoxb8 was a gift of Hans Häcker (USA, Tennessee, Memphis). This work was supported institutional grants from INSERM, CNRS and Aix-Marseille University to the CIML and the Agence Nationale de la Recherche grants: ANR-16-CE15-0023-01 (SalmoTubes) and ANR-10-INBS-04 (Investments for the Future, France-BioImaging).
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Fang, Z., Lagier, M., Méresse, S. (2021). Production of Murine Macrophages from Hoxb8-Immortalized Myeloblasts: Utility and Use in the Context of Salmonella Infection. In: Schatten, H. (eds) Salmonella. Methods in Molecular Biology, vol 2182. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0791-6_11
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DOI: https://doi.org/10.1007/978-1-0716-0791-6_11
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