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Chimeric DNA–RNA Guide RNA Designs

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CRISPR Guide RNA Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2162))

Abstract

CRISPR-associated nuclease (Cas) has been widely applied to modify the genomes of various cell types. As RNA-guided endonucleases, Cas enzymes can target different genomic sequences simply by changing the guide sequence of the CRISPR RNA (crRNA) or single guide RNA (sgRNA). Recent studies have demonstrated that DNA-RNA chimeric crRNA or sgRNA can efficiently guide the Cas9 protein for genome editing with reduced off-target effects. This chapter aims to describe a procedure for using chimeric RNA to modify the genomes of mammalian cells.

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Author information

Authors and Affiliations

  1. Department of Pathology, Medical Research Institute, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China

    Shuhan Lu & Hao Yin

  2. Department of Urology, Medical Research Institute, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China

    Shuhan Lu & Hao Yin

  3. Medical Research Institute, Renmin Hospital of Wuhan University, Wuhan, China

    Ying Zhang

Authors
  1. Shuhan Lu
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  2. Ying Zhang
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  3. Hao Yin
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Corresponding authors

Correspondence to Ying Zhang or Hao Yin .

Editor information

Editors and Affiliations

  1. Radcliffe Department of Medicine, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK

    Tudor A. Fulga

  2. Radcliffe Department of Medicine, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK

    David J. H. F. Knapp

  3. Radcliffe Department of Medicine, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK

    Quentin R. V. Ferry

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Lu, S., Zhang, Y., Yin, H. (2021). Chimeric DNA–RNA Guide RNA Designs. In: Fulga, T.A., Knapp, D.J.H.F., Ferry, Q.R.V. (eds) CRISPR Guide RNA Design. Methods in Molecular Biology, vol 2162. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0687-2_6

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  • DOI: https://doi.org/10.1007/978-1-0716-0687-2_6

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0686-5

  • Online ISBN: 978-1-0716-0687-2

  • eBook Packages: Springer Protocols

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