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Aging

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Sean P. Curran

    1. Leonard Davis School of Gerontology, University of Southern California, Los Angeles, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2144)

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About this book

This volume details the impact of aging on biological system and to understand the basis of this diversity with the goal of elucidating mechanisms to optimize health. Chapters guide readers through different animal models and methodologies to examine aging at the organism, tissue, cellular, and molecular levels across the lifespan. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge,  Aging: Methods and Protocols aims to ensure successful results in the further study of this vital field.

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Keywords

Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Measuring the Replicative Lifespan of Saccharomyces cerevisiae Using the HYAA Microfluidic Platform

    • Ruofan Yu, Myeong Chan Jo, Weiwei Dang
    Pages 1-6

  3. Analysis of Lifespan in C. elegans: Low- and High-Throughput Approaches

    • Adam B. Cornwell, Andrew V. Samuelson
    Pages 7-27

  4. A Simple Apparatus for Individual C. elegans Culture

    • William E. Pittman, Drew B. Sinha, Holly E. Kinser, Nisha S. Patil, Eric S. Terry, Isaac B. Plutzer et al.
    Pages 29-45

  5. Analysis of Drosophila melanogaster Lifespan

    • Gary N. Landis, Devon Doherty, John Tower
    Pages 47-56

  6. Mouse Fitness as Determined Through Treadmill Running and Walking

    • Joseph C. Reynolds, Changhan Lee
    Pages 57-65

  7. Human Population Genetics in Aging Studies for Molecular Biologists

    • Brendan Miller, Amin Haghani, Jennifer Ailshire, T. Em Arpawong
    Pages 67-76

  8. Design and Analysis of Pharmacological Studies in Aging

    • Khalyd J. Clay, Michael Petrascheck
    Pages 77-89

  9. Methods for Assessing Fertility in C. elegans from a Single Population

    • Chia-An Yen, Sean P. Curran
    Pages 91-102

  10. Metabolic Assessment of Lipid Abundance and Distribution

    • James D. Nhan, Sean P. Curran
    Pages 103-110

  11. Quantitative Profiling of Lipid Species in Caenorhabditis elegans with Gas Chromatography–Mass Spectrometry

    • Elizabeth C. Pino, Alexander A. Soukas
    Pages 111-123

  12. Assessing Insulin and Glucose Tolerance in the Aging Mouse

    • Roberta Buono, Sebastian Brandhorst
    Pages 125-129

  13. High-Throughput Assessment of Changes in the Caenorhabditis elegans Gut Microbiome

    • Fan Zhang, Jessica L. Weckhorst, Adrien Assié, Anastasia S. Khodakova, Mario Loeza-Cabrera, Daniela Vidal et al.
    Pages 131-144

  14. Measurements of Innate Immune Function in C. elegans

    • Kyle J. Foster, Deborah L. McEwan, Read Pukkila-Worley
    Pages 145-160

  15. Measuring Phagocytosis in Bone Marrow-Derived Macrophages and Peritoneal Macrophages with Aging

    • Ryan Lu, Nirmal K. Sampathkumar, Bérénice A. Benayoun
    Pages 161-170

  16. Chromatin Immunoprecipitation from Caenorhabditis elegans Somatic Cells

    • Mintie Pu, Siu Sylvia Lee
    Pages 171-175

  17. Transcriptional Profiling of C. elegans Adult Cells and Tissues with Age

    • Rachel Kaletsky, Coleen T. Murphy
    Pages 177-186

  18. Assessing Tissue-Specific Autophagy Flux in Adult Caenorhabditis elegans

    • Jessica T. Chang, Malene Hansen, Caroline Kumsta
    Pages 187-200

  19. Assay Development and Measurement of the Aging Biomarker Humanin

    • Brendan Miller, Junxiang Wan
    Pages 201-209

  20. Staining and Quantification of β-Amyloid Pathology in Transgenic Mouse Models of Alzheimer’s Disease

    • Amy Christensen, Christian J. Pike
    Pages 211-221
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Editors and Affiliations

  • Leonard Davis School of Gerontology, University of Southern California, Los Angeles, USA

    Sean P. Curran

Bibliographic Information

  • Book Title: Aging

  • Book Subtitle: Methods and Protocols

  • Editors: Sean P. Curran

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0592-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0591-2Published: 15 May 2020

  • Softcover ISBN: 978-1-0716-0594-3Published: 16 May 2021

  • eBook ISBN: 978-1-0716-0592-9Published: 14 May 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 277

  • Number of Illustrations: 11 b/w illustrations, 37 illustrations in colour

  • Topics: Molecular Medicine, Cell Biology

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