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Roles of MicroRNAs in Esophageal Squamous Cell Carcinoma Pathogenesis

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Book cover Esophageal Squamous Cell Carcinoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2129))

Abstract

MicroRNAs (miRNAs) are 20–22 nucleotides long single-stranded noncoding RNAs. They regulate gene expression posttranscriptionally by base pairing with the complementary sequences in the 3′-untranslated region of their targeted mRNA. Aberrant expression of miRNAs leads to alterations in the expression of oncogenes and tumor suppressors, thereby affecting cellular growth, proliferation, apoptosis, motility, and invasion capacity of gastrointestinal cells, including cells of esophageal squamous cell carcinoma (ESCC). Thus, alterations in miRNAs expression associated with the pathogenesis and progression of ESCC. In addition, expression profiles of miRNAs correlated with various clinicopathological factors, including pathological stages, histological differentiation, invasion, metastasis of cancer, as well as survival rates and therapy response of patients with ESCC. Consequently, expression profiles of miRNAs could be useful as diagnostic, prognostic, and prediction biomarkers in ESCC. Herein, we describe the quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and microarray methods for detection and quantitate miRNAs in ESCC. In addition, we summarize the roles of miRNAs in ESCC pathogenesis, progression, and prognosis.

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Authors and Affiliations

  1. Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Queensland, Australia

    Farhadul Islam & Vinod Gopalan

  2. Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh

    Farhadul Islam

  3. Cancer Molecular Pathology, School of Medicine, Griffith University, Gold Coast, Queensland, Australia

    Alfred K. Lam

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  1. Farhadul Islam
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  2. Vinod Gopalan
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  3. Alfred K. Lam
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Correspondence to Alfred K. Lam .

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Editors and Affiliations

  1. Cancer Molecular Pathology, School of Medicine, Griffith University, Gold Coast, QLD, Australia

    Alfred K. Lam

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Islam, F., Gopalan, V., Lam, A.K. (2020). Roles of MicroRNAs in Esophageal Squamous Cell Carcinoma Pathogenesis. In: Lam, A. (eds) Esophageal Squamous Cell Carcinoma. Methods in Molecular Biology, vol 2129. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0377-2_18

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  • DOI: https://doi.org/10.1007/978-1-0716-0377-2_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0376-5

  • Online ISBN: 978-1-0716-0377-2

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