Abstract
For a long time, conventional breeding methods have been used to obtain pure, 100% homozygous lines for hybrid seed production in crops of agronomic interest. However, by doubled haploid technology, it is possible to produce 100% homozygous plants derived from precursors of male gametophytes (androgenesis), to accelerate the production of pure lines, which implies important time and cost savings. In this chapter, a protocol for anther culture in eggplant is described, from donor plant growth conditions to regeneration and acclimation of doubled haploid plants, as well as a description of how to analyze ploidy levels of regenerated plants.
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Acknowledgments
This work was supported by grant AGL2017-88135-R to J.M.S.S. from MINECO jointly funded by FEDER.
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Calabuig-Serna, A., Porcel, R., Corral-Martínez, P., Seguí-Simarro, J.M. (2020). Anther Culture in Eggplant (Solanum melongena L.). In: Bayer, M. (eds) Plant Embryogenesis. Methods in Molecular Biology, vol 2122. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0342-0_20
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DOI: https://doi.org/10.1007/978-1-0716-0342-0_20
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