Abstract
Neotyphodium species are seed-transmitted endophytic fungi that form mutualistic (symbiotic) associations with grasses of the subfamily Pooideae, and their presence can increase stress tolerance of host grasses. Italian (annual) ryegrass (Lolium multiflorum) clones infected with N. occultans were pollinated by pollen from annual ryegrass and perennial ryegrass (L. perenne) using detached panicles, and from tall fescue (Festuca arundinacea) by conventional bagging, as part of studies on the use of this endophytic fungus to enhance productivity of this important forage grass and also of interspecific/intergeneric hybrids. In mating involving L. multiflorum, 64–100% seed transmission ratios of the fungus were observed among 13 cross-combinations, along with one exceptional case of no infection. In mating with L. perenne 53–100% transmission was observed, whereas less than 30% transmission was observed in crosses involving F. arundinacea. The results indicated that the symbiont can be seed transmitted through mating using detached panicles, and the possibility of poor compatibility between N. occultans and some L. multiflorum genotypes as well as with L .perenne and F. arundinacea.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Clay K, Schardl CL (2002) Evolutionary origins and ecological consequences of endophyte symbiosis with grasses. Am Nat 160 (Suppl): S99–S127
Jauhar PP(1993)Cytogenetics of the Festuca–Loium complex: relevance to breeding Springer, Berlin HeidelbergGermany
Koga H, Christensen MJ, Bennett RJ (1993) Incompatibility of some grass-Acremonium endophyte associations Mycol Res 97: 1237–1244
Latch GCM, Christensen MJ (1985) Artificial infection of grass with endophytes. Ann Appl Biol 107: 17–24
Moon CD, Schardl CL, Christensen MJ (2000) The evolutionary origins of Epichloë endophytes from annual ryegrass Mycologia 92: 1103–1118
Omacini M, Chaneton EJ, Ghersa CM, Müller CB (2001) Symbiotic fungal endophytes control insect host–parasite interaction webs. Nature 406: 78–81
Omacini M, Chaneton EJ, Ghersa CM, Otero P (2004) Do foliar endophytes affect grass litter decomposition? A microcosm approach using Lolium multiflorum. Oikos 104: 581–590
Shiba T, Sasaki T, Sugawara K, Arakawa A, Kanda K (2007) N-formylloline, a chemical produced within endophyte infected pasture grasses, can disrupt the growth of rice leaf bug, Trigonotylus caelestialum. Grassl Sci 53 (Suppl.): 358–359 (In Japanese. Translated title by the present authors)
Shimizu N, Ohsugi R (1977) Development and germination behaviour of seed produced by culture of cut ear in pasture plants. II. The optimum culture condition on Italian and perennial ryegrasses. Bull Natl Grassl Res Inst 11: 34–46
Sugawara K, Ohkubo H, Yamashita M, Mikoshiba Y (2004a) Flowers for Neotyphodium endophytes detection: a new method for detection from grasses Mycoscience 45: 222–226
Sugawara K, Shiba T, Yamashita M (2004b) Neotyphodium research and application in Japan In: Roberts CA, West CP, Spiers DE (eds), Neotyphodium in Cool-Season Grasses: Current Research and ApplicationsBlackwell, Ames, IA, pp. 55–63
Sugawara K, Inoue T, Yamashita M, Ohkubo H (2006) Distribution of the endophytic fungus, Neotyphodium occultans in naturalized Italian ryegrass in western Japan and its production of bioactive alkaloids known to repel insect pests Grassl Sci 52: 147–154
Sugawara K, Ohkubo H, Tsukiboshi T (2007) Distribution and morphology of Neotyphodium endophytes in apical meristems of host plants as observed using differential interference contrast microscopy. Proceedings of the 6th International Symposium on Fungal Endophytes of Grasses, pp. 139–141
Stewart AV (1987) Plant breeding aspects of ryegrasses (Lolium sp.) infected with endophytic fungi. PhD thesis, Lincoln College
Stewart AV (1997) Observations on maintaining endophyte during backcrossing of endophyte from perennial ryegrass to annual ryegrasses. In: Bacon CW, Hill NS (eds), Neotyphodium/Grass Interactions (Proceedings of the 3rd International Symposium on Acremonium/grass Interactions)Plenum Press, New York, NY, p. 279
Wafford DS, Frakes RV, Chilcote DO (1986) A detached culm technique for seed production of tall fescue in isolation from foreign pollen sources. Crop Sci 26: 193–195
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2009 Springer Science + Business Media, LLC
About this paper
Cite this paper
Sugawara, K., Arakawa, A., Shiba, T., Ohkubo, H., Tsukiboshi, T. (2009). Seed Transmission of Endophytic Fungus, Neotyphodium occultans, in Cross Breeding of Italian Ryegrass (Lolium multiflorum) Using Detached Panicle Culture, and Comparison with Situations in Interspecific/Intergeneric crossings including Festuca species. In: Molecular Breeding of Forage and Turf. Springer, New York, NY. https://doi.org/10.1007/978-0-387-79144-9_27
Download citation
DOI: https://doi.org/10.1007/978-0-387-79144-9_27
Publisher Name: Springer, New York, NY
Print ISBN: 978-0-387-79143-2
Online ISBN: 978-0-387-79144-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)