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The Genetics and Biochemistry of Maize Zein Storage Proteins

  • Chapter
Handbook of Maize

Zeins, the most abundant proteins in the maize seed, have been studied for over 100 years. We have learned a great deal about the structure, synthesis and genetic regulation of these proteins, making them a valuable model system to study storage protein synthesis. In this review, we explore longstanding questions and controversies regarding zeins in light of new information from genomic sequencing, improved protein detection methods and molecular characterization of mutants.

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References

  • Anfinsen, C.B. (1973) Principles that govern the folding of protein chains. Science 181: 223–230.

    Article  PubMed  CAS  Google Scholar 

  • Argos, P., K. Pedersen, M.D. Marks, and B.A. Larkins (1982) A structural model for maize zein proteins. J. Biol. Chem. 257: 9984–9990.

    PubMed  CAS  Google Scholar 

  • Azevedo, R.A., C. Damerval, J. Landry, P.J. Lea, C.M. Bellato, L.W. Meinhardt, M. Le Guilloux, S.Delhaye, A.A. Toro, S.A. Gaziola, and B.D. Berdejo (2003) Regulation of maize lysinemetabolism and endosperm protein synthesis by opaque and floury mutations. Eur J Biochem. 270: 4898–908.

    Article  PubMed  CAS  Google Scholar 

  • Bagga, S., H. Adams, J.D. Kemp, and C. Sengupta-Gopalan (1995) Accumulation of 15-kilodalton zein in novel protein bodies in transgenic tobacco. Plant Physiol. 107: 13–23.

    PubMed  CAS  Google Scholar 

  • Bagga, S., H.P. Adams, F.D. Rodriguez, J.D. Kemp, and C. Sengupta-Gopalan (1997) Coexpression of the maize delta-zein and beta-zein genes results in stable accumulation of delta-zein in endoplasmic reticulum-derived protein bodies formed by beta-zein. Plant Cell 9: 1683–1696.

    Article  PubMed  CAS  Google Scholar 

  • Balconi, C., E. Rizzi, M. Motto, F. Salamini, and R.D. Thompson (1993) The accumulation of zein polypeptides and zein mRNA in cultured endosperms of maize is modulated by nitrogen supply. The Plant J. 3: 325–334.

    CAS  Google Scholar 

  • Bellucci, M., A. Alpini, F. Paolocci, L. Cong, and S. Arcioni (2000) Accumulation of maize ?-zein and ?-zein:KDEL to high levels in tobacco leaves and differential increase of BiP synthesis in transformants. Theor Appl Genet. 101: 796–804

    Article  CAS  Google Scholar 

  • Boston, R.S., E.B. Fontes, B.B. Shank, and R.L. Wrobel (1991) Increased expression of the maize immunoglobulin binding protein homolog b-70 in three zein regulatory mutants. Plant Cell 3: 497–505.

    Article  PubMed  CAS  Google Scholar 

  • Burr, B. and F.A. Burr (1976) Zein synthesis in maize endosperm by polyribosomes attached to protein bodies. Proc. Natl. Acad. Sci. U. S. A. 73: 515–519.

    Article  PubMed  CAS  Google Scholar 

  • Burr, B., F.A. Burr, T.P. St. John, M. Thomas, and R.W. Davis (1982) Zein storage protein gene family of maize. An assessment of heterogeneity with cloned messenger RNA sequences. J. Mol. Biol. 154: 33–49.

    CAS  Google Scholar 

  • Chui, C.F. and S.C. Falco (1995) A new methionine-rich seed storage protein from maize. Plant Physiol. 107: 291.

    Article  PubMed  CAS  Google Scholar 

  • Ciceri, P., S. Castelli, M. Lauria, B. Lazzari, A. Genga, L. Bernard, M. Sturaro, and A. Viotti (2000) Specific combinations of zein genes and genetic backgrounds influence the transcription of the heavy-chain zein genes in maize opaque-2 endosperms. Plant Physiol. 124: 451–460.

    Article  PubMed  CAS  Google Scholar 

  • Ciceri, P., F. Locatelli, A. Genga, A. Viotti, and R.J. Schmidt (1999) The activity of the maize Opaque2 transcriptional activator is regulated diurnally. Plant Physiol. 121: 1321–1328.

    Article  PubMed  CAS  Google Scholar 

  • Coleman, C.E., E.M. Herman, K. Takasaki, and B.A. Larkins (1996) The maize ?-zein sequesters a-zein and stabilizes its accumulation in protein bodies of transgenic tobacco endosperm. Plant Cell 8: 2335–2345.

    Article  PubMed  CAS  Google Scholar 

  • Coleman, C.E., M.A. Lopes, J.W. Gillikin, R.S. Boston, and B.A. Larkins (1995) A defective signal peptide in the maize high-lysine mutant floury 2. Proc. Natl. Acad. Sci. U. S. A. 92: 6828–6831.

    Article  PubMed  CAS  Google Scholar 

  • Consoli, L. and C. Damerval (2001) Quantification of individual zein isoforms resolved by two-dimensional electrophoresis: Genetic variability in 45 maize inbred lines. Electrophoresis 22: 2983–2989.

    Article  PubMed  CAS  Google Scholar 

  • Das, O.P. and J.W. Messing (1987) Allelic variation and differential expression at the 27-kilodalton zein locus in maize. Mol. Cell Biol. 7: 4490–4497.

    PubMed  CAS  Google Scholar 

  • Duvick, D.N. (1961) Protein granules of maize endosperm cells. Cereal Chem. 38: 374–385.

    CAS  Google Scholar 

  • Emrich, S.J., W.B. Barbazuk, L. Li, and P.S. Schnable (2007) Gene discovery and annotation using LCM-454 transcriptome sequencing. Genome Res. 17: 69–73.

    Article  PubMed  CAS  Google Scholar 

  • Esen, A. (1986) Separation of alcohol-soluble proteins (zeins) from maize into three fractions by differential solubility. Plant Physiol. 80: 623–627.

    Article  PubMed  CAS  Google Scholar 

  • Esen, A. (1987) A proposed nomenclature for the alcohol-soluble proteins (zeins) of maize (Zea mays-L). J. Cereal Sci. 5: 117–128.

    Article  CAS  Google Scholar 

  • Esen, A. and D.A. Stetler (1992) Immunocytochemical localization of the delta-zein in protein bodies of maize endosperm cells. Amer. J. Bot. 79: 243–248.

    CAS  Google Scholar 

  • Fontes, E.B., B.B. Shank, R.L. Wrobel, S.P. Moose, G.R. OBrian, E.T. Wurtzel, and R.S. Boston (1991) Characterization of an immunoglobulin binding protein homolog in the maize floury-2 endosperm mutant. Plant Cell 3: 483–496.

    Article  PubMed  CAS  Google Scholar 

  • Galante, E., A. Vitale, L. Manzocchi, C. Soave, and F. Salamini (1983) Genetic control of a membrane component and zein deposition in maize endosperm. Mol. Gen. Genet. 192: 316–321.

    Article  CAS  Google Scholar 

  • Garratt, R., G. Oliva, I. Caracelli, A. Leite, and P. Arruda (1993) Studies of the zein-like alpha-prolamins based on an analysis of amino acid sequences: implications for their evolution and three-dimensional structure. Proteins: Structure, Function and Genetics 15: 88–99.

    Article  CAS  Google Scholar 

  • Geetha, K.B., C.R. Lending, M.A. Lopes, J.C. Wallace, and B.A. Larkins (1991) opaque-2 modifiers increase ?-zein synthesis and alter its spatial distribution in maize endosperm. Plant Cell 3: 1207–1219.

    Article  PubMed  CAS  Google Scholar 

  • Geli, M.I., M. Torrent, and D. Ludevid (1994) Two structural domains mediate two sequential events in ?-zein targeting: protein endoplasmic reticulum retention and protein body formation. Plant Cell 6: 1911–1922.

    Article  PubMed  CAS  Google Scholar 

  • Geraghty, D., Peifer, M.A., Rubenstein, I. and Messing, J. (1981). The primary structure of a plant storage protein: Zein. Nucl. Acids Res. 9: 5163–5174.

    Article  CAS  Google Scholar 

  • Geraghty, D.E., J. Messing, and I. Rubenstein (1982) Sequence analysis and comparison of cDNAs of the zein multigene family. EMBO J. 1: 1329–1335.

    PubMed  CAS  Google Scholar 

  • Gianazza, E., V. Viglienghi, P.G. Righetti, F. Salamini, and C. Soave (1977) Amino acid composition of zein molecular components. Phytochem. 16: 315–317.

    Article  CAS  Google Scholar 

  • Gillikin, J.W., F. Zhang, C.E. Coleman, H.W. Bass, B.A. Larkins, and R.S. Boston (1997) A defective signal peptide tethers the floury-2 zein to the endoplasmic reticulum membrane. Plant Physiol. 114: 345–352.

    Article  PubMed  CAS  Google Scholar 

  • Habben, J.E., A.W. Kirleis, and B.A. Larkins (1993) The origin of lysine-containing proteins in opaque-2 maize endosperm. Plant Mol. Biol. 23: 825–838.

    Article  PubMed  CAS  Google Scholar 

  • Hagen, G. and I. Rubenstein (1981) Complex organization of zein genes in maize. Gene 13: 239–249.

    Article  PubMed  CAS  Google Scholar 

  • Hartings, H., M. Maddaloni, N. Lazzaroni, N. Di Fonzo, M. Motto, F. Salamini, and R. Thompson (1989) The O2 gene which regulates zein deposition in maize endosperm encodes a protein with structural homologies to transcriptional activators. EMBO J. 8: 2795–2801.

    PubMed  CAS  Google Scholar 

  • Hinnebusch, A.G. and K. Natarajan (2002) Gcn4p, a master regulator of gene expression, is controlled at multiple levels by diverse signals of starvation and stress. Eukaryot. Cell 1: 22–32.

    Article  PubMed  CAS  Google Scholar 

  • Holding, D.R., M.S. Otegui, B. Li, R.B. Meeley, T. Dam, B.G. Hunter, R. Jung, and B.A. Larkins (2007) The maize Floury1 gene encodes a novel endoplasmic reticulum protein involved in zein protein body formation. Plant Cell 19: 2569–2582.

    Article  PubMed  CAS  Google Scholar 

  • Hu, N.T., M.A. Peifer, G. Heidecker, J. Messing, and I. Rubenstein (1982) Primary structure of a genomic zein sequence in maize. EMBO J. 1: 1337–1342.

    PubMed  CAS  Google Scholar 

  • Huang, S., W.R. Adams, Q. Zhou, K.P. Malloy, D.A. Voyles, J. Anthony, A.L. Kriz, and M.H.Luethy (2004) Improving nutritional quality of maize proteins by expressing sense and antisense zein genes. J. Agric. Food Chem. 52: 1958–1964.

    Article  PubMed  CAS  Google Scholar 

  • Hunter, B.G., M.K. Beatty, G.W. Singletary, B.R. Hamaker, B.P. Dilkes, B.A. Larkins, and R. Jung (2002) Maize opaque endosperm mutations create extensive changes in patterns of gene expression. Plant Cell 14: 2591–2612.

    Article  PubMed  CAS  Google Scholar 

  • Kim, C.S., B.C. Gibbon, J.W. Gillikin, B.A. Larkins, R.S. Boston, and R. Jung (2006) The maize Mucronate mutation is a deletion in the 16-kDa ?-zein gene that induces the unfolded protein response. The Plant J. 48: 440–451.

    Article  CAS  Google Scholar 

  • Kim, C.S., B.G. Hunter, J. Kraft, R.S. Boston, S. Yans, R. Jung, and B.A. Larkins (2004) A defective signal peptide in a 19-kD alpha-zein protein causes the unfolded protein response and an opaque endosperm phenotype in the maize De*-B30 mutant. Plant Physiol. 134: 380–387.

    Article  PubMed  CAS  Google Scholar 

  • Kim, C.S., Y.-m. Woo, A.M. Clore, R.J. Burnett, N.P. Carneiro, and B.A. Larkins (2002) Zein protein interactions, rather than the asymmetric distribution of zein mRNAs on endoplasmic reticulum membranes, influence protein body formation in maize endosperm. Plant Cell 14: 655–672.

    Article  PubMed  CAS  Google Scholar 

  • Kirihara, J.A., J.B. Petri, and J. Messing (1988) Isolation and sequence of a gene encoding a methionine-rich 10-kDa zein protein from maize. Gene 71: 359–370.

    Article  PubMed  CAS  Google Scholar 

  • Kirst, M.E., D.J. Meyer, B.C. Gibbon, R. Jung, and R.S. Boston (2005) Identification and characterization of endoplasmic reticulum-associated degradation proteins differentially affected by endoplasmic reticulum stress. Plant Physiol. 138: 218–231.

    Article  PubMed  CAS  Google Scholar 

  • Kriz, A.L., R.S. Boston, and B.A. Larkins (1987) Structural and transcriptional analysis of DNA sequences flanking genes that encode 19 kilodalton zeins. Mol. Gen. Genet. 207: 90–98.

    Article  PubMed  CAS  Google Scholar 

  • Landry, J. and T. Moureaux (1970) Héterogénéite des glutélines du grain de mais: extraction sélective et composition en acidés aminés des trois fractions isolées. Bull. Soc. Chim. Biol. 52: 1021–1037.

    PubMed  CAS  Google Scholar 

  • Larkins, B.A. and A. Dalby (1975) In vitro synthesis of zein-like protein by maize polyribosomes. Biochem. Biophys. Res. Commun. 66: 1048–1054.

    Article  PubMed  CAS  Google Scholar 

  • Larkins, B.A. and W.J. Hurkman (1978) Synthesis and deposition of zein in protein bodies of maize endosperm. Plant Physiol. 62: 256–263.

    Article  PubMed  CAS  Google Scholar 

  • Larkins, B.A., K. Pedersen, A.K. Handa, W.J. Hurkman, and L.D. Smith (1979) Synthesis and processing of maize storage proteins in Xenopus laevis oocytes. Proc. Natl. Acad. Sci. U. S. A. 76: 6448-–6452.

    Article  PubMed  CAS  Google Scholar 

  • Larkins, B.A., J.C. Wallace, G. Galili, C.R. Lending, and E. Kawata (1989) Structural analysis and modification of maize storage proteins. Dev. Indust. Micro. 30: 203–209.

    CAS  Google Scholar 

  • Lawton, J.W. (2002) Zein: A history of processing and use. Cereal Chem. 79: 1–18.

    Article  CAS  Google Scholar 

  • Leaver, C.J. (1980) Genome Organization and Expression in Plants. Plenum Press, New York.

    Google Scholar 

  • Lee, K.H., R.A. Jones, A. Dalby, and C.Y. Tsai (1976) Genetic regulation of storage protein content in maize endosperm. Biochem. Genet. 14: 641–650.

    Article  PubMed  CAS  Google Scholar 

  • Lending, C.R., A.L. Kriz, B.A. Larkins, and C.E. Bracker (1988) Structure of maize protein bodies and immunocytochemical localization of zeins. Protoplasma 143: 51–62.

    Article  CAS  Google Scholar 

  • Lending, C.R. and B.A. Larkins (1989) Changes in the zein composition of protein bodies during maize endosperm development. Plant Cell 1: 1011–1023.

    Article  PubMed  CAS  Google Scholar 

  • Lending, C.R. and B.A. Larkins (1992) Effect of the floury-2 locus on protein body formation during maize endosperm development. Protoplasma 171: 123–133.

    Article  Google Scholar 

  • Lund, G., P. Ciceri, and A. Viotti (1995) Maternal-specific demethylation and expression of specific alleles of zein genes in the endosperm of Zea mays L. The Plant J. 8: 571–581.

    Article  CAS  Google Scholar 

  • Lund, G., M. Lauria, P. Guldberg, and S. Zaina (2003) Duplication-dependent CG suppression of the seed storage protein genes of maize. Genetics 165: 835–848.

    PubMed  CAS  Google Scholar 

  • Maier, U.G., J.W. Brown, C. Tologcyzki, and G. Feix (1987) Binding of a nuclear factor to a consensus sequence in the 5′ flanking region of zein genes from maize. EMBO J. 6: 17–22.

    PubMed  CAS  Google Scholar 

  • Marks, M.D. and B.A. Larkins (1982) Analysis of sequence microheterogeneity among zein messenger RNAs. J. Biol. Chem. 257: 9976–9983.

    PubMed  CAS  Google Scholar 

  • Marocco, A., A. Santucci, S. Cerioli, M. Motto, N. Di Fonzo, R. Thompson, and F. Salamini (1991) Three high-lysine mutations control the level of ATP-binding HSP70-like proteins in the maize endosperm. Plant Cell 3: 507–515.

    Article  PubMed  CAS  Google Scholar 

  • McKinney, L.L. (1958) Zein. In: The Encyclopedia of Chemistry (G.L. Clark, ed.). Reinhold, N.Y., pp. 319–320.

    Google Scholar 

  • Mertz, E.T., L.S. Bates, and O.E. Nelson (1964) Mutant gene that changes protein composition and increases lysine content of maize endosperm. Science 145: 279–280.

    Article  PubMed  CAS  Google Scholar 

  • Misra, P.S., E.T. Mertz, and D.V. Glover (1975) Characteristics of proteins in single and double endosperm mutants of maize. In: High Quality Protein Maize (Bauman, L. F., E. T. Mertz, A. Caballo, and E. W. Sprague, eds.) Dowden, Hutchinson and Ross, Inc., Stroudsburg, PA, pp. 291–305.

    Google Scholar 

  • Motto, M., M. Maddaloni, G. Ponziani, M. Brembilla, R. Marotta, N. Fonzo, C. Soave, R. Thompson, and F. Salamini (1988) Molecular cloning of the o2-m5 allele of Zea mays using transposon marking. Mol. Gen. Genet. 212: 488–494.

    Article  CAS  Google Scholar 

  • Nelson, O.E., E.T. Mertz, and L.S. Bates (1965) Second mutant gene affecting the amino acid pattern of maize endosperm proteins. Science 150: 1469–1470.

    Article  PubMed  CAS  Google Scholar 

  • Osborne, T.B. (1897) Amount and properties of the proteins of the maize kernel. J. Amer. Chem. Soc. 19: 525–532.

    Article  CAS  Google Scholar 

  • Osborne, T.B. (1908) Our present knowledge of plant proteins. Science 28: 417–427.

    Article  PubMed  Google Scholar 

  • Park, W.D., E.D. Lewis, and I. Rubenstein (1980) Heterogeneity of zein mRNA and protein in maize. Plant Physiol. 65: 98–106.

    Article  PubMed  CAS  Google Scholar 

  • Paulis, J.W., C. James, and J.S. Wall (1969) Comparison of glutelin proteins in normal and high-lysine corn. J. Agric. Food Chem. 17: 1301–1305.

    Article  CAS  Google Scholar 

  • Paulis, J.W. and J.S. Wall (1971) Fractionation and properties of alkylated-reduced corn glutelin proteins. Biochim. Biophys. Acta. 251: 57–69.

    PubMed  CAS  Google Scholar 

  • Pedersen, K., P. Argos, S.V. Naravana, and B.A. Larkins (1986) Sequence analysis and characteri zation of a maize gene encoding a high-sulfur zein protein of Mr 15,000. J. Biol. Chem. 261: 6279–6284.

    PubMed  CAS  Google Scholar 

  • Pedersen, K., K.S. Bloom, J.N. Anderson, D.V. Glover, and B.A. Larkins (1980) Analysis of the complexity and frequency of zein genes in the maize genome. Biochemistry 19: 1644–1650.

    Article  PubMed  CAS  Google Scholar 

  • Pedersen, K., J. Devereux, D.R. Wilson, E. Sheldon, and B.A. Larkins (1982) Cloning and sequence analysis reveal structural variation among related zein genes in maize. Cell 29: 1015–1026.

    Article  PubMed  CAS  Google Scholar 

  • Prat, S., J. Cortadas, P. Puigdomenech, and J. Palau (1985) Nucleic acid (cDNA) and amino acid sequences of the maize endosperm protein glutelin-2. Nucleic Acids Res. 13: 1493–1504.

    Article  PubMed  CAS  Google Scholar 

  • Prat, S., L. Perez-Grau, and P. Puigdomenech (1987) Multiple variability in the sequence of a family of maize endosperm proteins. Gene 52: 41–49.

    Article  PubMed  CAS  Google Scholar 

  • Pysh, L.D., M.J. Aukerman, and R.J. Schmidt (1993) OHP1: a maize basic domain/leucine zipper protein that interacts with Opaque2. Plant Cell 5: 227 #x2013;236.

    Article  PubMed  CAS  Google Scholar 

  • Randall, J.J., D.W. Sutton, S.F. Hanson, and J.D. Kemp (2005) BiP and zein binding domains within the delta zein protein. Planta 221: 656–666.

    Article  PubMed  CAS  Google Scholar 

  • Righetti, P.G., E. Gianazza, A. Viotti, and C. Soave (1977) Heterogeneity of storage proteins in maize. Planta 136: 115–123.

    Article  CAS  Google Scholar 

  • Schmidt, R.J., F.A. Burr, M.J. Aukerman, and B. Burr (1990) Maize regulatory gene opaque-2 encodes a protein with a “leucine-zipper ” motif that binds to zein DNA. Proc. Natl. Acad. Sci. U. S. A. 87: 46 –50.

    Article  PubMed  CAS  Google Scholar 

  • Schmidt, R.J., F.A. Burr, and B. Burr (1987) Transposon tagging and molecular analysis of the maize regulatory locus opaque-2. Science 238: 960 –963.

    Article  PubMed  CAS  Google Scholar 

  • Segal, G., R. Song, and J. Messing (2003) A new opaque variant of maize by a single dominant RNA-interference-inducing transgene. Genetics 165: 387 –397.

    PubMed  CAS  Google Scholar 

  • Shank, K.J., P. Su, I. Brglez, W.F. Boss, R.E. Dewey, and R.S. Boston (2001) Induction of lipid metabolic enzymes during the endoplasmic reticulum stress response in plants. Plant Physiol. 126: 267 –277.

    Article  PubMed  CAS  Google Scholar 

  • Soave, C., R. Reggiani, N. Di Fonzo, and F. Salamini (1981) Clustering of genes for 20 kd zein subunits in the short arm of maize chromosome 7. Genetics 97: 363 –377.

    PubMed  CAS  Google Scholar 

  • Soave, C. and F. Salamini (1984) Organization and regulation of zein in maize endosperm. Phil. Trans. R. Soc. Lond. B. 304: 341 –347.

    Article  CAS  Google Scholar 

  • Soave, C., N. Surman, A. Viotti, and F. Salamini (1978) Linkage relationships between regulatory and structural gene loci involved in zein synthesis in maize. Theor. Appl. Genet. 52: 263 –267.

    Article  Google Scholar 

  • Sodek, L. and C.M. Wilson (1971) Amino acid composition of proteins isolated from normal, opaque-2 and floury-2 corn endosperms by a modified Osborne procedure. J. Agric. Food Chem. 19: 1144 –1150.

    Article  CAS  Google Scholar 

  • Song, R., V. Llaca, E. Linton, and J. Messing (2001) Sequence, regulation, and evolution of the maize 22-kD alpha zein gene family. Genome Res. 11: 1817 –1825.

    PubMed  CAS  Google Scholar 

  • Song, R. and J. Messing (2003) Gene expression of a gene family in maize based on noncollinear haplotypes. Proc. Natl. Acad. Sci. U. S. A. 100: 9055 –9060.

    Article  PubMed  CAS  Google Scholar 

  • Spena, A., A. Viotti, and V. Pirrotta (1983) Two adjacent genomic zein sequences: structure, organization and tissue-specific restriction pattern. J. Mol. Biol. 169:799 –811.

    Article  PubMed  CAS  Google Scholar 

  • Spena, A., A. Viotti, and V. Pirrotta (1982) A homologous repetitive block structure underlies the heterogeneity of heavy and light chain zein genes. EMBO J. 1: 1589 –1594.

    PubMed  CAS  Google Scholar 

  • Swarup, S., Timmermans, M.C.P., Chaudhuri, S., and Messing, J. (1995). Determinants of the high-methionine trait in wild and exotic germplasm may have escaped selection during early cultivation of maize. The Plant J. 8: 359 –368.

    Article  CAS  Google Scholar 

  • Tatham, A.S., J.M. Field, V.J. Morris, K.J. IœAnson, L. Cardle, M.J. Dufton, and P.R. Shewry (1993) Solution conformational analysis of the alpha-zein proteins of maize. J. Biol. Chem. 268: 26253 –26259.

    PubMed  CAS  Google Scholar 

  • Thompson, G.A., D.R. Siemieniak, L.C. Sieu, J.L. Slightom, and B.A. Larkins (1992) Sequence analysis of linked maize 22 kDa alpha-zein genes. Plant Mol. Biol. 18: 827 –833.

    Article  PubMed  CAS  Google Scholar 

  • Ueda, T., Wang, Z., Pham, N., and Messing, J. (1994). Identification of a transcriptional activator- binding element in the 27-kilodalton zein promoter, the -300 element. Mol. Cell. Biol. 14: 4350 –4359.

    PubMed  CAS  Google Scholar 

  • Valentini, G., C. Soave, and E. Ottaviano (1979) Chromosomal location of zein genes in Zea mays. Heredity 42: 33 –46.

    Article  CAS  Google Scholar 

  • Vicente-Carbajosa, J., S.P. Moose, R.L. Parsons, and R.J. Schmidt (1997) A maize zinc-finger protein binds the prolamin box in zein gene promoters and interacts with the basic leucine zipper transcriptional activator Opaque2. Proc. Natl. Acad. Sci. U. S. A. 94: 7685 –7690.

    Article  PubMed  CAS  Google Scholar 

  • Viotti, A., E. Sala, P. Alberi, and C. Soave (1978) Heterogeneity of zein synthesized in vitro. Plant Sci. Lett. 13.

    Google Scholar 

  • Viotti, A., E. Sala, R. Marotta, P. Alberi, C. Balducci, and C. Soave (1979) Genes and mRNAs coding for zein polypeptides in Zea mays. Eur. J. Biochem. 102: 211 –222.

    Article  PubMed  CAS  Google Scholar 

  • Wang, Z., Ueda, T., and Messing, J. (1998). Characterization of the maize prolamin box-binding factor-1 (PBF-1) and its role in developmental regulation of the zein multigene family. Gene 223: 321 –332.

    Article  PubMed  CAS  Google Scholar 

  • Wang, Z., and Messing, J. (1998). Modulation of gene expression by DNA-protein and protein- protein interactions in the promoter region of the zein multigene family. Gene 223: 333 –345.

    Article  PubMed  CAS  Google Scholar 

  • Washida, H., A. Sugino, J. Messing, A. Esen, and T.W. Okita (2004) Asymmetric localization of seed storage protein RNAs to distinct subdomains of the endoplasmic reticulum in developing maize endosperm cells. Plant Cell Physiol. 45: 1830 –1837.

    Article  PubMed  CAS  Google Scholar 

  • Weinand, U., C. Bruschke, and G. Feix (1979) Cloning of double stranded DNAs derived from polysomal mRNA of maize endosperm: isolation and characterisation of zein clones. Nucleic Acids Res. 6: 2707 –2715.

    Article  PubMed  CAS  Google Scholar 

  • Wienand, U., P. Langridge, and G. Feix (1981) Isolation and characterization of a genomic sequence of maize coding for a zein gene. Mol. Gen. Genet. 182: 440 –444.

    Article  CAS  Google Scholar 

  • Wilson, D.R. and B.A. Larkins (1984) Zein gene organization in maize and related grasses. J. Mol. Evol. 20: 330 –340.

    Article  PubMed  CAS  Google Scholar 

  • Wolf, M.J., U. Khoo, and H.L. Seckinger (1969) Distribution and subcellular structure of endosperm protein in varieties of ordinary and high-lysine maize. Cereal Chem. 46: 253 –263.

    CAS  Google Scholar 

  • Woo, Y.M., D.W. Hu, B.A. Larkins, and R. Jung (2001) Genomics analysis of genes expressed in maize endosperm identifies novel seed proteins and clarifies patterns of zein gene expression. Plant Cell 13: 2297 –2317.

    Article  PubMed  CAS  Google Scholar 

  • Zhang, F. and R.S. Boston (1992) Increases in binding protein (BiP) accompany changes in protein body morphology in three high-lysine mutants of maize. Protoplasma 171: 142 –152.

    Article  CAS  Google Scholar 

  • Zimmerberg, J. and M.M. Kozlov (2006) How proteins produce cellular membrane curvature. Nat.Rev. Mol. Cell Biol. 7: 9 –19.

    Article  PubMed  CAS  Google Scholar 

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Authors
  1. Rebecca S. Boston
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  2. Brian A. Larkins
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Editor information

Editors and Affiliations

  1. Department of Genetics, University of Georgia, Athens, GA, 30602–7223, USA

    Jeffrey L. Bennetzen

  2. USDA Plant Gene Expression Center, 800 Buchanan Street, Albany, CA, 94710, USA

    Sarah Hake

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© 2009 Springer Science + Business Media, LLC

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Boston, R.S., Larkins, B.A. (2009). The Genetics and Biochemistry of Maize Zein Storage Proteins. In: Bennetzen, J.L., Hake, S. (eds) Handbook of Maize. Springer, New York, NY. https://doi.org/10.1007/978-0-387-77863-1_36

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