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Amplified DNA Biosensors

  • Chapter
Functional Nucleic Acids for Analytical Applications

Part of the book series: Integrated Analytical Systems ((ANASYS))

Abstract

Amplified detection of DNA is a central research topic in modern bioanalytical science. Electronic or optical transduction of DNA recognition events provides readout signals for DNA biosensors. Amplification of the DNA analysis is accomplished by the coupling of nucleic acid-functionalized enzymes or nucleic acid-functionalized nanoparticles (NP) as labels for the DNA duplex formation. This chapter discusses the amplified amperometric analysis of DNA by redox enzymes, the amplified optical sensing of DNA by enzymes or DNAzymes, and the amplified voltammetric, optical, or microgravimetric analysis of DNA using metallic or semiconductor nanoparticles. Further approaches to amplify DNA detection involve the use of micro-carriers of redox compounds as labels for DNA complex formation on electrodes, or the use of micro-objects such as liposomes, that label the resulting DNA complexes on electrodes and alter the interfacial properties of the electrodes. Finally, DNA machines are used for the optical detection of DNA, and the systems are suggested as future analytical procedures that could substitute the polymerase chain reaction (PCR) process.

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Acknowledgments

Our research on amplified DNA analyses is supported by the Israel Ministry of Science and Technology, and by the Johnson & Johnson Corporation.

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Authors and Affiliations

  1. Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

    Itamar Willner, Bella Shlyahovsky, Bilha Willner & Maya Zayats

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  1. Itamar Willner
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  2. Bella Shlyahovsky
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  3. Bilha Willner
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  4. Maya Zayats
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Editors and Affiliations

  1. Department of Biochemistry and Biomedical Sciences, McMaster University Hamilton, Ontario, Canada

    Li Yingfu

  2. Department of Chemistry, University of Illinois at Urbana-Champaign Urbana, Illinois, USA

    Lu Yi

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© 2009 Springer Science+Business Media, LLC

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Willner, I., Shlyahovsky, B., Willner, B., Zayats, M. (2009). Amplified DNA Biosensors. In: Yingfu, L., Yi, L. (eds) Functional Nucleic Acids for Analytical Applications. Integrated Analytical Systems. Springer, New York, NY. https://doi.org/10.1007/978-0-387-73711-9_8

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