Abstract
In this chapter we will try to provide a more intuitive (and less mathematical) insight into image formation and practical image restoration by deconvolution methods. The mathematics of image formation and deconvolution microscopy have been described in greater detail elsewhere (see Chapters 11, 22, 23, and 24), so we will limit our discussion to fundamental issues and gloss over most of the mathematics of image restoration. We will also focus on practical ways of assessing microscope performance and getting the best possible data before applying more sophisticated image processing methods than are usually seen in the literature.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Abbe, E., 1873, Beitrage zur theorie des microscopes und der mikroskopischen wahrnehmung, Schultzes Arch f. Mikr. Anat. 9:413:468.
Agard, D.A., Hiraoka, Y., Shaw, P., and Sedat, J.W., 1989, Fluorescence microscopy in three dimensions, Methods Cell Biol. 30:353–377.
Born, M., and Wolf, E., 1980, Principles of Optics: Electromagnetic Theory of Propagation, Interference and Diffraction of Light, Pergamon Press, Oxford, England.
Cagnet, M., Francon, M., and Thrierr, J.C., 1962, An Atlas of Optical Phenomena, Springer-Verlag, Berlin.
Denk, W., Strickler, J.H., and Webb, W.W., 1990, Two-photon laser scanning fluorescence microscopy, Science 248:73–76.
Holmes, T.J., and O’Connor, N.J., 2000, Blind deconvolution of 3D transmitted light brightfield micrographs, J. Microsc. 200:114–127.
Jansson, P.A., Hunt, R.H., and Plyler, E.K., 1970, Resolution enhancement of spectra, J. Opt. Soc. Am. 60:596–599.
Keller, H.E., 1995, Objective lenses for confocal microscopy, In: Handbook of Biological Confocal Microscopy (J.B. Pawley, ed.), Plenum Press, New York, pp. 111–126 or Chapter 7, this volume.
Lucy, L.B., 1974, An iterative technique for the rectification of observed distributions, Astronomy J. 79:745–765.
Mullikin, J.C., Van Vliet, L.J., Netten, H., Boddeke, F.R., van der Feltz, G.W., and Young, I.T., 1994, Methods for CCD camera characterization. Proc. SPIE 2173:73–84.
Pawley, J.B., 1995, Handbook of Biological Confocal Microscopy, 2nd ed., Plenum Press, New York.
Press, W.H., Teukolsky, S.A., Vetterling, W.T., and Flannery, B.P., 2002, Numerical recipes in C: The art of scientific computing. Cambridge University Press, Cambridge, UK, 1–970; available free, on-line at www.library.cornell.edu.
Richardson, W.H., 1972, Bayesian-based iterative method of image restoration, J. Opt. Soc. Am. 62:55–59.
Shaw, P.J., and Rawlins, D.J., 1991, Three-dimensional fluorescence microscopy, Prog. Biophys. Mol. Biol. 56:187–213.
Tikhonov, A.N., and Arsenin, V.I., 1977, Solutions of Ill-Posed Problems, V.H. Winston, Washington, DC.
van der Voort, H.T.M., and Brakenhoff, G.J., 1990, 3-D image formation in high-aperture fluorescence confocal microscopy: A numerical analysis, J. Microsc. 158:43–54.
van Kempen, G.M., 1999, Image restoration in fluorescence microscopy, In: Advanced School for Computing and Imaging, Delft University of Technology, Delft, The Netherlands, p. 161.
van Kempen, G.M.P., and van Vliet, L.J., 2000, Background estimation in nonlinear image restoration; J. Opt. Soc. Am. A-Opt. and Im. Sci. 17(3):425–433.
van Kempen, G.M., van Vliet, L., Verveer, P., and van der Voort, H., 1997, A quantitative comparison of image restoration methods for confocal
microscopy, J. Microsc. 185:354–365.
Wilson, T., 1990, Confocal microscopy, In: Confocal Miscrocopy (T. Wilson, ed), Academic Press, London, pp. 1–64.
Young, I.T., 1989, Image fidelity: Characterizing the imaging transfer function, Methods Cell Biol. 30:1–45.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2006 Springer Science+Business Media, LLC
About this chapter
Cite this chapter
Cannell, M.B., McMorland, A., Soeller, C. (2006). Image Enhancement by Deconvolution. In: Pawley, J. (eds) Handbook Of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-45524-2_25
Download citation
DOI: https://doi.org/10.1007/978-0-387-45524-2_25
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-387-25921-5
Online ISBN: 978-0-387-45524-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)