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Synthesis of Hybrid Gold Nanoparticle (AuNP) Functionalized Superparamagnetic Nanoparticles (SPMNPs) for Efficient Coupling of Biomolecules

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2125))

Abstract

Recently, we reported our methodology for isolating plasma membrane and lysosome from eukaryotic cell using superparamagnetic nanoparticles (SPMNPs). Here in this article, we report a step-by-step protocol for synthesis of hybrid gold nanoparticle (AuNP), surface functionalization of AuNPs on superparamagnetic nanoparticles (SPMNPs), and potential use of hybrid AuNP-SPMNP for efficient coupling of biomolecules.

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References

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Acknowledgments

This work was supported by Envirotransgene® Global, Chennai, India, and Deepak Thimiri Consulting Group (DTCG®), Chennai, India. Authors thank the infrastructural support from Bannari Amman Institute of Technology, India.

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Correspondence to Deepak B. Thimiri Govinda Raj .

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Thimiri Govinda Raj, D.B., Khan, N.A., Venkatachalam, S., Arumugam, S., Palaniswamy, S. (2019). Synthesis of Hybrid Gold Nanoparticle (AuNP) Functionalized Superparamagnetic Nanoparticles (SPMNPs) for Efficient Coupling of Biomolecules. In: Turksen, K. (eds) Stem Cell Nanotechnology. Methods in Molecular Biology, vol 2125. Humana, New York, NY. https://doi.org/10.1007/7651_2019_254

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  • DOI: https://doi.org/10.1007/7651_2019_254

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0359-8

  • Online ISBN: 978-1-0716-0360-4

  • eBook Packages: Springer Protocols

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