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Isolation and Cultivation of Skin-Derived Precursors

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1879))

Abstract

Skin-derived precursors (SKPs) have been shown recently to be capable of inducing hair genesis and hair follicle regeneration. Here, we describe a protocol for SKP isolation and culture based on our experience and previous publications.

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References

  1. Ito M et al (2007) Wnt-dependent de novo hair follicle regeneration in adult mouse skin after wounding. Nature 447(7142):316–320

    Article  CAS  Google Scholar 

  2. Morris RJ et al (2004) Capturing and profiling adult hair follicle stem cells. Nat Biotechnol 22:411–417

    Article  CAS  Google Scholar 

  3. Jahoda CA et al (1984) Induction of hair growth by implantation of cultured dermal papilla cells. Nature 311:560–562

    Article  CAS  Google Scholar 

  4. Biernaskie J et al (2009) SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells. Cell Stem Cell 5(6):610–623

    Article  CAS  Google Scholar 

  5. Wang X et al (2016) Hair follicle and sebaceous gland de novo regeneration with cultured epidermal stem cells and skin-derived precursors. Stem Cells Transl Med 5(12):1695–1706

    Article  CAS  Google Scholar 

  6. Toma JG et al (2001) Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol 3:778–784

    Article  CAS  Google Scholar 

  7. Toma JG et al (2005) Isolation and characterization of multipotent skin-derived precursors from human skin. Stem Cells 23:727–737

    Article  CAS  Google Scholar 

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Correspondence to Yaojiong Wu .

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Wang, X., Dong, S., Wu, Y. (2018). Isolation and Cultivation of Skin-Derived Precursors. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 1879. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_141

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  • DOI: https://doi.org/10.1007/7651_2018_141

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8869-3

  • Online ISBN: 978-1-4939-8870-9

  • eBook Packages: Springer Protocols

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