Abstract
The choice of the fluorophore to be used in super-resolution far-field nanoscopy is an essential parameter that typically determines the quality of the resulting image. To overcome the diffraction limit, different super-resolution techniques exploit different optical and photochemical phenomena, imposing method-specific requirements for useful probes. The choice of the fluorophore is even more intimately tied to performance for super-resolution imaging in live cells, where both photophysical properties and target-specific labeling inside the cell are essential. In this chapter, we highlight the advances in fluorophore development for a range of existing super-resolution techniques. We discuss the requirements these distinct methods place on the fluorophores in order to achieve optimal resolution, particularly as these methods move toward imaging living cells beyond the diffraction limit.
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Yushchenko, D.A., Bruchez, M.P. (2012). Tailoring Fluorescent Labels for Far-Field Nanoscopy. In: Tinnefeld, P., Eggeling, C., Hell, S. (eds) Far-Field Optical Nanoscopy. Springer Series on Fluorescence, vol 14. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2011_35
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DOI: https://doi.org/10.1007/4243_2011_35
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