Skip to main content

Detection of Spots in 2-D Electrophoresis Gels by Symmetry Features

  • Conference paper

Part of the book series: Lecture Notes in Computer Science ((LNIP,volume 3686))

Abstract

We have implemented an algorithm for detection and segmentation of protein spots in 2-D gel electrophoresis images using symmetry derivative features computed using low level image processing operations. The implementation was compared with a previously published Watershed segmentation and a commercial software. Our algorithm was found to yield segmentation results that were either better than or comparable to the other solutions while having fewer free parameters and a low computational cost.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bettens, E., Scheunders, P., Van Dyck, D., Moens, L., Van Osta, P.: Electrophoresis 18, 792–798 (1997)

    Google Scholar 

  2. Bigün, J., Bigün, T., Nilsson, K.: IEEE-PAMI 26, 1590–1605 (2004)

    Google Scholar 

  3. Persson, M., Bigün, J., Bigun, J., Gustavsson, T. (eds.): SCIA 2003. LNCS, vol. 2749, pp. 520–525. Springer, Heidelberg (2003)

    Google Scholar 

  4. Görg, A., Weiss, W., Dunn, M.J.: Proteomics 4, 3665–3685 (2004)

    Google Scholar 

  5. Garrels, J.I.: J. Biol. Chem.  264, 5269–5282 (1989)

    Google Scholar 

  6. Appel, R.D., Vargas, J.R., Palagi, P.M., Walther, D., et al.: Electrophoresis 18, 2724–2734 (1997)

    Google Scholar 

  7. Baker, M., Busse, H., Vogt, M.: Medical Imaging 2000: Image Processing, San Diego, SPIE, Bellingham, 2979, pp. 426-436 (2000)

    Google Scholar 

  8. Rogers, M., Graham, J., Tonge, R.P.: Proteomics 6, 887–896 (2003)

    Google Scholar 

  9. Pleissner, K.-P., Hoffman, F., Kriegel, K., Wenk, C., Wegner, S., Sahlström, S., Oswald, H., Alt, H., Fleck, E.: Electrophoresis 20, 755–765 (1999)

    Google Scholar 

  10. Mo, X., Wilson, R.: In: Bigun, J., Gustavsson, T. (eds.) SCIA 2003. LNCS, vol. 2749, pp. 430–437. Springer, Heidelberg (2003)

    Google Scholar 

  11. Vincent, L., Soille, P.: IEEE PAMI 13, 583–598 (1991)

    Google Scholar 

  12. Nilsson, K., Bigün, J.: Pattern Recognition Letters 24, 2135–2144 (2003)

    Google Scholar 

  13. Premaratne, H.L., Bigün, J.: Pattern Recognition 37, 2081–2089 (2004)

    Google Scholar 

  14. Funahashi, K.: Neural Networks 2(3), 183–192

    Google Scholar 

  15. Riedmiller, M., Braun, H.: Proc. of the IEEE Intl. Conf. on Neural Networks, San Francisco (1993)

    Google Scholar 

  16. Rogers, M., Graham, J., Tonge, R.P.: Proteomics 6, 879–886 (2003)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2005 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Persson, M., Bigun, J. (2005). Detection of Spots in 2-D Electrophoresis Gels by Symmetry Features. In: Singh, S., Singh, M., Apte, C., Perner, P. (eds) Pattern Recognition and Data Mining. ICAPR 2005. Lecture Notes in Computer Science, vol 3686. Springer, Berlin, Heidelberg. https://doi.org/10.1007/11551188_47

Download citation

  • DOI: https://doi.org/10.1007/11551188_47

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-28757-5

  • Online ISBN: 978-3-540-28758-2

  • eBook Packages: Computer ScienceComputer Science (R0)

Publish with us

Policies and ethics