This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Gurib-Fakim A (2006) Medicinal plants: Traditions of yesterday and drugs of tomorrow. Mol Aspects Med 27(1):1–93
Bafi-Yeboa NFA, Arnason JT, Baker J, Smith ML (2005) Antifungal constituents of Northern prickly ash Zanthoxylum americanum Mill. Phytomedicine 12(5):370–377
Yoon SB, Lee YJ, Park SK, Kim HC, Bae H, Kim HM, Ko SG, Choi HY, Oh MS, Park W (2009) Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages. J Ethnopharmacol 125(2):286–290
Thuong PT, Pokharel YR, Lee MY, Kim SK, Bae K, Su ND, Oh WK, Kang KW (2009) Dual Anti-oxidative Effects of Fraxetin Isolated from Fraxinus rhinchophylla. Biol Pharm Bull 32(9):1527–1532
Yeo SK, Ooi LG, Lim TJ, Liong MT (2009) Antihypertensive properties of plant-based prebiotics. Int J Mol Sci 10(8):3517–3530
Efferth T, Li PCH, Konkimalla VSB, Kaina B (2007) From traditional Chinese medicine to rational cancer therapy. Trends Mol Med 13(8):353–361
Graham JG, Quinn ML, Fabricant DS, Farnsworth NR (2000) Plants used against cancer—an extension of the work of Jonathan Hartwell. J Ethnopharmacol 73(3):347–377
Bedoya LM, Sanchez-Palomino S, Abad MJ, Bermejo P, Alcami J (2001) Anti-HIV activity of medicinal plant extracts. J Ethnopharmacol 77(1):113–116
Liu CZ, Towler MJ, Medrano G, Cramer CL, Weathers PJ (2009) Production of mouse interleukin-12 is greater in tobacco hairy roots grown in a mist reactor than in an airlift reactor. Biotechnol Bioeng 102(4):1074–1086
Woodard SL, Wilken LR, Barros GOF, White SG, Nikolov ZL (2009) Evaluation of monoclonal antibody and phenolic extraction from transgenic Lemna for purification process development. Biotechnol Bioeng 104(3):562–571
Verpoorte R, Contin A, Memelink J (2002) Biotechnology for the production of plant secondary metabolites. Phytochem Rev 1(1):13–25
Chilton MD, Tepfer DA, Petit A, David C, Cassedelbart F, Tempe J (1982) Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant-root cells. Nature 295(5848):432–434
Mishra BN, Ranjan R (2008) Growth of hairy-root cultures in various bioreactors for the production of secondary metabolites. Biotechnol Appl Biochem 49:1–10
Hu ZB, Du M (2006) Hairy root and its application in plant genetic engineering. J Integr Plant Biol 48:121–127
Hamill JD, Robins RJ, Parr AJ, Evans DM, Furze JM, Rhodes MJC (1990) Over-expressing a yeast ornithine decarboxylase gene in transgenic Roots of Nicotiana rustica Can Lead to Enhanced Nicotine Accumulation. Plant Mol Biol 15(1):27–38
Hashimoto T, Yun DJ, Yamada Y (1993) Production of tropane alkaloids in genetically engineered root cultures. Phytochemistry 32(3):713–718
Moyano E, Jouhikainen K, Tammela P, Palazon J, Cusido RM, Pinol MT, Teeri TH, Oksman-Caldentey KM (2003) Effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of Datura metel and Hyoscyamus muticus. J Exp Bot 54(381):203–211
Zhang L, Ding RX, Chai YR, Bonfill M, Moyano E, Oksman-Caldentey KM, Xu TF, Pi Y, Wang ZN, Zhang HM, Kai GY, Liao ZH, Sun XF, Tang KX (2004) Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures, In: Proceedings of the national academy of sciences of the United States of America 101(17):6786–6791
Jouhikainen K, Lindgren L, Jokelainen T, Hiltunen R, Teeri TH, Oksman-Caldentey KM (1999) Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering. Planta 208(4):545–551
Li FX, Jin ZP, Zhao DX, Cheng LQ, Fu CX, Ma FS (2006) Overexpression of the Saussurea medusa chalcone isomerase gene in Saussurea involucrata hairy root cultures enhances their biosynthesis of apigenin. Phytochemistry 67(6):553–560
Huang TK, McDonald KA (2012) Bioreactor systems for in vitro production of foreign proteins using plant cell cultures. Biotechnol Adv 30(2):398–409
Sevon N, Drager B, Hiltunen R, OksmanCaldentey KM (1997) Characterization of transgenic plants derived from hairy roots of Hyoscyamus muticus. Plant Cell Rep 16(9):605–611
Shanks JV, Morgan J (1999) Plant hairy root culture. Curr Opin Biotechnol 10(2):151–155
Kim Y, Wyslouzil BE, Weathers PJ (2002a) Invited review: secondary metabolism of hairy root cultures in bioreactors. In Vitro Cell Dev Biol Plant 38(1):1–10
Eibl R, Eibl D (2008) Design of bioreactors suitable for plant cell and tissue cultures. Phytochem Rev 7:593–598
Paek KY, Chakrabarty D, Hahn EJ (2005) Application of bioreactor systems for large scale production of horticultural and medicinal plants. Plant Cell, Tissue Organ Cult 81(3):287–300
Curtis WR (2000) Bioreactor growth of hairy roots. In: Spier R (ed) Encyclopedia of Cell Technology. John Wiley and Sons, New York, pp 827–841
Jung G, Tepfer D (1987) Use of Genetic-Transformation by the Ri T-DNA of Agrobacterium rhizogenes to stimulate biomass and tropane alkaloid production in Atropa belladonna and Calystegia sepium roots grown-invitro. Plant Sci 50(2):145–151
Wilson PDG, Hilton MG, Robins RJ, Rhodes MJC (1987) Fermentation studies of transformed root cultures. In: international conference on bioreactors and biotransformations, Elsevier, London, pp 38–51
Doran PM (1999) Design of mixing systems for plant cell suspensions in stirred reactors. Biotechnol Prog 15(3):319–335
Kondo O, Honda H, Taya M, Kobayashi T (1989) Comparison of growth-properties of carrot hairy root in various bioreactors. Appl Microbiol Biotechnol 32(3):291–294
Uozumi N, Kohketsu K, Kobayashi T (1993) Growth and kinetic-parameters of Ajuga hairy root in fed-batch culture on monosaccharide medium. J Chem Technol Biotechnol 57(2):155–161
Davioud E, Kan C, Hamon J, Tempe J, Husson HP (1989) Production of indole alkaloids by In vitro root cultures from Catharanthus trichophyllus. Phytochemistry 28(10):2675–2680
Jeong GT, Park DH, Hwang B, Woo JC (2003) Comparison of growth characteristics of Panax ginseng hairy roots in various bioreactors. Appl Biochem Biotechnol 105:493–503
Srivastava S, Srivastava AK (2012b) In vitro Azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Appl Biochem Biotechnol 166(2):365–378
Srivastava S, Srivastava AK (2012a) Azadirachtin production by hairy root cultivation of Azadirachta indica in a modified stirred tank reactor. Bioprocess Biosystems Engineering 35(9):1549–1553
Choi Y, Kim Y, Paek K (2006) Types and designs of bioreactors for hairy culture. In: Duttagupta S, Ibaraki Y (eds) Plant tissue culture engineering. Springer, Dordrecht, pp 161–172
Kwok KH, Doran PM (1995) Kinetic and stoichiometric analysis of hairy roots in a segmented bubble-column reactor. Biotechnol Prog 11(4):429–435
Kanokwaree K, Doran PM (1998) Application of membrane tubing aeration and perfluorocarbon to improve oxygen delivery to hairy root cultures. Biotechnol Prog 14(3):479–486
Min JY, Jung HY, Kang SM, Kim YD, Kang YM, Park DJ, Prasad DT, Choi MS (2007) Production of tropane alkaloids by small-scale bubble column bioreactor cultures of Scopolia parviflora adventitious roots. Bioresour Technol 98(9):1748–1753
Yoshikawa T, Furuya T (1987) Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes. Plant Cell Reps 6(6):449–453
Taya M, Yoyama A, Kondo O, Kobayashi T, Matsui C (1989) Growth-characteristics of plant hairy roots and their cultures in bioreactors. J Chem Eng Japan 22(1):84–89
Rodriguez-Mendiola MA, Stafford A, Cresswell R, Ariascastro C (1991) Bioreactors for growth of plant-roots. Enzym Microb Technol 13(9):697–702
Sauerwein M, Yamazaki T, Shimomura K (1991) Hernandulcin in hairy root cultures of Lippia dulcis. Plant Cell Rep 9(10):579–581
Shimomura K, Sudo H, Saga H, Kamada H (1991) Shikonin production and secretion by hairy root cultures of Lithospermum erythrorhizon. Plant Cell Rep 10(6–7):282–285
Sudo H, Yamakawa T, Yamazaki M, Aimi N, Saito K (2002) Bioreactor production of camptothecin by hairy root cultures of Ophiorrhiza pumila. Biotechnol Lett 24(5):359–363
Abbasi BH, Liu R, Saxena PK, Liu CZ (2009) Cichoric acid production from hairy root cultures of Echinacea purpurea grown in a modified airlift bioreactor. J Chem Technol Biotechnol 84(11):1697–1701
Kintzios S, Makri O, Pistola E, Matakiadis T, Shi HP, Economou A (2004) Scale-up production of puerarin from hairy roots of Pueraria phaseoloides in an airlift bioreactor. Biotechnol Lett 26(13):1057–1059
Katuri SR, Ranjan R, Khanna R (2011) Mathematical modeling of mist bioreactor for the growth of hairy roots In: National workshop-cum-conference on recent trends in Mathematics and Computing
Ramakrishnan D, Salim J, Curtis WR (1994) Inoculation and tissue distribution in pilot-scale plant-root culture bioreactors. Biotechnol Tech 8(9):639–644
Wyslouzil BE, Whipple M, Chatterjee C, Walcerz DB, Weathers PJ, Hart DP (1997) Mist deposition onto hairy root cultures: Aerosol modeling and experiments. Biotechnol Prog 13(2):185–194
Towler MJ, Kim Y, Wysiouzil BE, Correll MJ, Weathers PJ (2006) Design, development, and applications of mist bioreactors for micropropagation and hairy root culture. In: Gupta SD, Ibaraki Y (eds) Plant tissue culture engineering. Springer, Netherlands, pp 119–134
Sivakumar G, Liu CZ, Towler M, Weathers PJ (2010b) Biomass production of hairy roots of Artemisia annua and Arachis hypogaea in a scaled-up mist bioreactor. Biotechnol Bioeng 107(5):802–813
Kochan E, Krolicka A, Chmiel A (2012) Growth and Ginsenoside production in Panax quinquefolium hairy roots cultivated in flasks and nutrient sprinkle bioreactor. Acta Physiologiae Plantarum 34(4):1513–1518
Grzegorczyk I, Wysokinska H (2010) Antioxidant compounds in Salvia officinalis L. shoot and hairy root cultures in the nutrient sprinkle bioreactor. Acta Societatis Botanicorum Poloniae 79(1):7–10
Kuzma L, Bruchajzer E, Wysokinska H (2009) Methyl jasmonate effect on diterpenoid accumulation in Salvia sclarea hairy root culture in shake flasks and sprinkle bioreactor. Enzym Microb Technol 44(6–7):406–410
Williams GRC, Doran PM (1999) Investigation of liquid-solid hydrodynamic boundary layers and oxygen requirements in hairy root cultures. Biotechnol Bioeng 64(6):729–740
Ramakrishnan D, Curtis WR (2004) Trickle-bed root culture bioreactor design and scale-up: Growth, fluid-dynamics, and oxygen mass transfer. Biotechnol Bioeng 88(2):248–260
Kim Y, Wyslouzil BE, Weathers PJ (2001) A comparative study of mist and bubble column reactors in the in vitro production of Artemisinin. Plant Cell Rep 20(5):451–455
Kim YJ, Weathers PJ, Wyslouzil BE (2002c) Growth of Artemisia annua hairy roots in liquid- and gas-phase reactors. Biotechnol Bioeng 80(4):454–464
Suresh B, Bais HP, Raghavarao KSMS, Ravishankar GA, Ghildyal NP (2005) Comparative evaluation of bioreactor design using Tagetes patula L. hairy roots as a model system. Process Biochem 40(5):1509–1515
Wilson PDG (1997) The pilot-scale cultivation of transformed roots. In: Doran PM (ed) Hairy roots: culture and applications. Harwood Academic, Amsterdam, pp 190–197
Eibl R, Eibl D (2006) Design and use of the wave bioreactor for plant cell culture. In: Gupta SD, Ibaraki Y (eds) Plant tissue culture engineering. Springer, Netherlands, pp 203–227
Asplund PT, Curtis WR (2001) Intrinsic oxygen use kinetics of transformed plant root culture. Biotechnol Prog 17(3):481–489
Kim Y, Wyslouzil BE, Weathers PJ (2002b) Invited review: secondary metabolism of hairy root cultures in bioreactors. In vitro Cell Dev Biol Plant 38(1):1–10
Bordonaro JL, Curtis WR (2000) Inhibitory role of root hairs on transport within root culture bioreactors. Biotechnol Bioeng 70(2):176–186
Shiao TL, Doran PM (2000) Root hairiness: effect on fluid flow and oxygen transfer in hairy root cultures. J Biotechnol 83(3):199–210
Dhaouadi H, Poncin S, Hornut JM, Midoux N (2008) Gas-liquid mass transfer in bubble column reactor: analytical solution and experimental confirmation. Chem Eng Process 47(4):548–556
Ducos JP, Terrier B, Courtois D (2009) Disposable bioreactors for plant micropropagation and mass plant cell culture. Dispos Bioreactors 115:89–115
Carvalho EB, Curtis WR (1998) Characterization of fluid-flow resistance in root cultures with a convective flow tubular bioreactor. Biotechnol Bioeng 60(3):375–384
Hitaka Y, Kino-Oka M, Taya M, Tone S (1997) Effect of liquid flow on culture of red beet hairy roots in single column reactor. J Chem Eng Jpn 30(6):1070–1075
Kino-Oka R, Hitaka Y, Taya M, Tone S (1999) High-density culture of red beet hairy roots by considering medium flow condition in a bioreactor. Chem Eng Sci 54(15–16):3179–3186
Shiao TI, Ellis MH, Dolferus R, Dennis ES, Doran PM (2002) Overexpression of alcohol dehydrogenase or pyruvate decarboxylase improves growth of hairy roots at reduced oxygen concentrations. Biotechnol Bioeng 77(4):455–461
Dilorio AA, Cheetham RD, Weathers PJ (1992) Carbon-dioxide improves the growth of hairy roots cultured on solid medium and in nutrient mists. Appl Microbiol Biotechnol 37(4):463–467
Wyslouzil BE, Waterbury RG, Weathers PJ (2000) The growth of single roots of Artemisia annua in nutrient mist reactors. Biotechnol Bioeng 70(2):143–150
Sung LS, Huang SY (2000) Headspace ethylene accumulation on Stizolobium hassjoo hairy root culture producing L-3,4-dihydroxyphenylalanine. Biotechnology Letters 22(10):875–878
Abbasi BH, Tian CL, Murch SJ, Saxena PK, Liu CZ (2007) Light-enhanced caffeic acid derivatives biosynthesis in hairy root cultures of Echinacea purpurea. Plant Cell Rep 26(8):1367–1372
Wang YC, Zhang HX, Zhao B, Yuan XF (2001) Improved growth of Artemisia annua L hairy roots and artemisinin production under red light conditions. Biotechnol Lett 23(23):1971–1973
Zhong JJ, Seki T, Kinoshita S, Yoshida T (1991) Effect of light irradiation on Anthocyanin production by suspended culture of Perilla frutescens. Biotechnol Bioeng 38(6):653–658
Taya M, Sato H, Kinooka M, Tone S (1994) Characterization of Pak-bung green hairy roots cultivated under light irradiation. J Ferment Bioeng 78(1):42–48
Bhadra R, Morgan JA, Shanks JV (1998) Transient studies of light-adapted cultures of hairy roots of Catharanthus roseus: Growth and indole alkaloid accumulation. Biotechnol Bioeng 60(6):670–678
Jacob A, Malpathak N (2004) Green hairy root cultures of Solanum khasianum clarke—a new route to in vitro Solasodine production. Curr Sci 87(10):1442–1447
Flores H, Medina-Bolivar H (1993) Root cultures and plant natural products: unearthing the hidden half of plant metabolism. In: Plant tissue culture and biotechnology. Balaban Publisher, UK, pp 59–74
Liu CZ, Guo C, Wang YC, Ouyang F (2002) Effect of light irradiation on hairy root growth and Artemisinin biosynthesis of Artemisia annua L. Process Biochem 38(4):581–585
Yu KW, Murthy HN, Hahn EJ, Paek KY (2005) Ginsenoside production by hairy root cultures of Panax ginseng: influence of temperature and light quality. Biochem Eng J 23(1):53–56
Yu SX, Kwok KH, Doran PM (1996) Effect of sucrose, exogenous product concentration, and other culture conditions on growth and steroidal alkaloid production by Solanum aviculare hairy roots. Enzym Microb Technol 18(4):238–243
Hilton MG, Rhodes MJC (1990) Growth and Hyoscyamine production of hairy root cultures of Datura stramonium in a modified stirred tank reactor. Appl Microbiol Biotechnol 33(2):132–138
Sivakumar G, Yu KW, Hahn EJ, Paek KY (2005) Optimization of organic nutrients for ginseng hairy roots production in large-scale bioreactors. Curr Sci 89(4):641–649
Wilhelmson A, Hakkinen ST, Kallio PT, Oksman-Caldentey KM, Nuutila AM (2006) Heterologous expression of Vitreoscilla hemoglobin (VHb) and cultivation conditions affect the alkaloid profile of Hyoscyamus muticus hairy roots. Biotechnol Prog 22(2):350–358
Weathers PJ, Hemmavanh DD, Walcerz DB, Cheetham RD, Smith TC (1997) Interactive effects of nitrate and phosphate salts, sucrose, and inoculum culture age on growth and sesquiterpene production in Artemisia annua hairy root cultures. In vitro Cell Dev Biol Plant 33(4):306–312
Amdoun R, Khelifi L, Khelifi-Slaoui M, Amroune S, Benyoussef EH, Thi DV, Assaf-Ducrocq C, Gontier E (2009) Influence of minerals and elicitation on Datura stramonium L. tropane alkaloid production: Modelization of the in vitro biochemical response. Plant Sci 177(2):81–87
Cui XH, Chakrabarty D, Lee EJ, Paek KY (2010) Production of adventitious roots and secondary metabolites by Hypericum perforatum L in a bioreactor. Bioresour Technol 101(12):4708–4716
Sreedhar RV, Roohie K, Maya P, Venkatachalam L, Bhagyalakshmi N (2009) Biotic elicitors enhance flavour compounds during accelerated curing of vanilla beans. Food Chem 112(2):461–468
Vasconsuelo A, Boland R (2007) Molecular aspects of the early stages of elicitation of secondary metabolites in plants. Plant Sci 172(5):861–875
Georgiev MI, Pavlov AI, Bley T (2007) Hairy root type plant in vitro systems as sources of bioactive substances. Appl Microbiol Biotechnol 74(6):1175–1185
Pitta-Alvarez SI, Spollansky TC, Giulietti AM (2000) The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzym Microb Technol 26(2–4):252–258
Satdive RK, Fulzele DP, Eapen S (2007) Enhanced production of Azadirachtin by hairy root cultures of Azadirachta indica A Juss by elicitation and media optimization. J Biotechnol 128(2):281–289
Rahimi S, Hasanloo T, Najafi F, Khavari-Nejad RA (2011) Methyl jasmonate influence on Silymarin production and plant stress responses in Silybum marianum hairy root cultures in a bioreactor. Nat Prod Res 26(18):1662–1667
Putalun W, Luealon W, De-Eknamkul W, Tanaka H, Shoyama Y (2007) Improvement of Artemisinin production by Chitosan in hairy root cultures of Artemisia annua L. Biotechnol Lett 29(7):1143–1146
Savitha BC, Thimmaraju R, Bhagyalakshmi N, Ravishankar GA (2006) Different biotic and abiotic elicitors influence Betalain production in hairy root cultures of Beta vulgaris in shake-flask and bioreactor. Process Biochem 41(1):50–60
Bhagwath SG, Hjortso MA (2000) Statistical analysis of elicitation strategies for Thiarubrine A production in hairy root cultures of Ambrosia artemisiifolia. J Biotechnol 80(2):159–167
Lin LD, Wu JY (2002) Enhancement of shikonin production in single- and two-phase suspension cultures of Lithospermum erythrorhizon cells using low-energy ultrasound. Biotechnol Bioeng 78(1):81–88
Lin LD, Wu JY, Ho KP, Qi SY (2001) Ultrasound-induced physiological effects and secondary metabolite (Saponin) production in Panax ginseng cell cultures. Ultrasound Med Biol 27(8):1147–1152
Wu J, Lin L (2003) Enhancement of Taxol production and release in Taxus chinensis cell cultures by ultrasound, methyl jasmonate and in situ solvent extraction. Appl Microbiol Biotechnol 62(2–3):151–155
Chisti Y (2003) Sonobioreactors: using ultrasound for enhanced microbial productivity. Trends Biotechnol 21(2):89–93
Kumar A, Kumaresan T, Pandit AB, Joshi JB (2006) Characterization of flow phenomena induced by ultrasonic horn. Chem Eng Sci 61(22):7410–7420
Cao CQ, Dong SQ, Zhao YA, Guo QJ (2010) Experimental and numerical research for fluidization behaviors in a gas-solid acoustic fluidized bed. AICHE J 56(7):1726–1736
Laborde JL, Hita A, Caltagirone JP, Gerard A (2000) Fluid dynamics phenomena induced by power ultrasounds. Ultrasonics 38(1–8):297–300
Kim S, Hopper E, Hjortso M (1995) Hairy root-growth models—effect of different branching patterns. Biotechnol Prog 11(2):178–186
Lenk F, Vogel M, Bley T, Steingroewer J (2012) Automatic image recognition to determine morphological development and secondary metabolite accumulation in hairy root networks. Eng Life Sci 12(5):1–7
Bastian P, Chavarria-Krauser A, Engwer C, Jager W, Marnach S, Ptashnyk M (2008) Modelling in vitro growth of dense root networks. J Theor Biol 254(1):99–109
Prince CL, Bringi V, Shuler ML (1991) Convective mass-transfer in large porous biocatalysts—plant organ-cultures. Biotechnol Prog 7(2):195–199
Ranjan R, Khanna R, Mishra BN (2011) Sustained operation of nutrient mist reactor to grow hairy roots. Asia-Pacific J Chem Eng 6(1):23–28
Sowana DD, Williams DRG, Dunlop EH, Dally BB, O’Neill BK, Fletcher DF (2001) Turbulent shear stress effects on plant cell suspension cultures. Chem Eng Res Des 79(A8):867–875
Atta A, Roy S, Nigam KDP (2010) A two-phase Eulerian approach using relative permeability concept for modeling of hydrodynamics in trickle-bed reactors at elevated pressure. Chem Eng Res Des 88(3):369–378
Merchuk J, Garcia-Camacho F, Molina-Grima E (2007) Photobioreactor design and fluid dynamics. Chem Biochem Eng Quarterly 21(4):345–355
Yu G, Li YC, Shen GM, Wang WL, Lin C, Wu HX, Chen ZS (2009) A novel method using CFD to optimize the inner structure parameters of flat photobioreactors. J Appl Phycol 21(6):719–727
Dhotre MT, Ekambara K, Joshi JB (2004) CFD simulation of sparger design and height to diameter ratio on gas hold-up profiles in bubble column reactors. Exp Thermal Fluid Sci 28(5):407–421
Zhong C, Yuan YJ (2009) Responses of Taxus cuspidata to hydrodynamics in bubble column bioreactors with different sparging nozzle sizes. Biochem Eng J 45(2):100–106
Ding J, Wang X, Zhou XF, Ren NQ, Guo WQ (2010) CFD optimization of continuous stirred-tank (CSTR) reactor for biohydrogen production. Bioresour Technol 101(18):7005–7013
Liu R, Sun W, Liu CZ (2011a) Computational fluid dynamics modeling of mass-transfer behavior in a bioreactor for hairy root culture. II. Analysis of ultrasound-intensified process. Biotechnol Prog 27(6):1672–1679
Liu R, Sun W, Liu CZ (2011b) Computational fluid dynamics modeling of mass transfer behavior in a bioreactor for hairy root culture. I. Model development and experimental validation. Biotechnol Prog 27(6):1661–1671
Ñopo L, Woffenden BJ, Reed DG, Buswell S, Zhang C, Medina-Bolivar F (2012) Super-promoter: TEV, a powerful gene expression system for tobacco hairy Roots. In: Lorence Argelia (ed) Recombinant gene expression: reviews and protocols, methods in molecular biology. Springer, Science + Business Media, pp 501–526
Patil P, Desai N, Govindwar S, Jadhav JP, Bapat V (2009) Degradation analysis of reactive red 198 by hairy roots of Tagetes patula L. (Marigold). Planta 230(4):725–735
Alderete LGS, Talano MA, Ibáñez SG, Purro S, Agostini E, Milrad SR, Medina MI (2009) Establishment of transgenic tobacco hairy roots expressing basic peroxidises and its application for phenol removal. J Biotechnol 139(4):273–279
Angelini VA, Orejas J, Medina MI, Agostini E (2011) Scale up of 2,4-dichlorophenol removal from aqueous solutions using Brassica napus hairy roots. J Hazard Mater 185(1):269–274
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Stiles, A.R., Liu, CZ. (2013). Hairy Root Culture: Bioreactor Design and Process Intensification. In: Doran, P. (eds) Biotechnology of Hairy Root Systems. Advances in Biochemical Engineering/Biotechnology, vol 134. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2013_181
Download citation
DOI: https://doi.org/10.1007/10_2013_181
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-39018-0
Online ISBN: 978-3-642-39019-7
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)