Conclusions
The work presented herein provides a general overview of caspase involvement in the apoptotic pathways and methods available for their inhibition. Genetic and chemical approaches may be utilized to lengthen batch times of cultured mammalian cells and in some instances, mutational analyses of genetic inhibitors are an effective means to obtain higher cell viabilities in culture. If these approaches to manipulate caspase activity in cell lines are applied in conjunction with improvements in bioreactor design, culture medium, cell line development, and expression vector design, optimum biopharmaceutical yield will be possible. Such advancements may lead to a more cost-effective biotechnology operation with recombinant protein yields surpassing those previously obtained.
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Sauerwald, T.M., Betenbaugh, M.J. (2004). The Role of Caspases in Apoptosis and Their Inhibition in Mammalian Cell Culture. In: Al-Rubeai, M., Fussenegger, M. (eds) Cell Engineering. Cell Engineering, vol 4. Springer, Dordrecht. https://doi.org/10.1007/1-4020-2217-4_7
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