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Effects of N deprivation and nitrate pulses on NRT1 and NRT2 transcript levels and nitrate influx rate in Brassica napus L.

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Plant Nutrition

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 92))

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Abstract

A model has been proposed by Clarkson (1986) in which nitrate uptake by higher plants is down-regulated under conditions of high internal N. This model was investigated in Brassica napus L. by measuring the activity of low and high affinity transport systems (HATS and LATS) in relation to mRNA abundance of inducible nitrate transporter genes BnNRT1 and BnNRT2 in roots. Induction effects of nitrate were examined by supplying N-deprived plants with nitrate pulses 12 h before measuring influx and 4 h before assaying mRNA levels. N deprivation decreased nitrate influx from both low and high external concentrations with no significant changes in the relative abundance of BnNRT1 and BnNRT2 transcripts. Nitrate pulses increase the NO3? influx of the HATS and the mRNA expression of BnNRT2 gene with the increasing duration of N deprivation but had no effect on LATS activity and BnNRT1 mRNA abundance.

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References

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Author information

Authors and Affiliations

  1. U.M.R INRA / U.C.B.N. Laboratoire de Physiologie et Biochimie Végétales Université de Caen, IRBA, F-14032, Caen Cedex

    S. Faure, E. Le Deunff, P. Lainé & A. Ourry

  2. Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion, SY23 3EB, UK

    J. H. Macduff

Authors
  1. S. Faure
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  2. E. Le Deunff
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  3. P. Lainé
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  4. J. H. Macduff
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  5. A. Ourry
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Editor information

Editors and Affiliations

  1. University of Hannover, Hannover, Germany

    W. J. Horst  & M. K. Schenk  & 

  2. University of Kassel, Witzenhausen, Germany

    A. Bürkert

  3. University of Göttingen, Göttingen, Germany

    N. Claassen  & H. Flessa  & 

  4. University of Tübingen, Tübingen, Germany

    W. B. Frommer  & N. v. Wirén  & 

  5. University of Bonn, Bonn, Germany

    H. Goldbach

  6. Hydro Agri, Pflanzenernährung und Umweltforschung, Dülmen, Germany

    H. -W. Olfs

  7. University of Hohenheim, Stuttgart, Germany

    V. Römheld

  8. University of Kiel, Kiel, Germany

    B. Sattelmacher

  9. TU München, Freising-Weihenstephan, Germany

    U. Schmidhalter

  10. University of Gießen, Gießen, Germany

    S. Schubert

  11. University of Halle-Wittenberg, Halle, Germany

    L. Wittenmayer

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© 2001 Kluwer Academic Publishers

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Faure, S., Le Deunff, E., Lainé, P., Macduff, J.H., Ourry, A. (2001). Effects of N deprivation and nitrate pulses on NRT1 and NRT2 transcript levels and nitrate influx rate in Brassica napus L.. In: Horst, W.J., et al. Plant Nutrition. Developments in Plant and Soil Sciences, vol 92. Springer, Dordrecht. https://doi.org/10.1007/0-306-47624-X_101

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  • DOI: https://doi.org/10.1007/0-306-47624-X_101

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-7105-2

  • Online ISBN: 978-0-306-47624-2

  • eBook Packages: Springer Book Archive

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