Conclusion
Both the in vitro and in vivo data reported here strongly support the hypothesis that Hha and H-NS interact with DNA to generate a nucleoprotein complex that influences gene expression. The Hha-H-NS complex seems to be mainly responsible for the thermoosmotic modulation of the expression of the hly operon of plasmid pHly152. Binding sites for Hha and H-NS along the regulatory region of the hly operon, as well as the topological changes mediated by the interaction of both proteins with DNA, are currently being investigated.
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© 2002 Kluwer Academic Publishers
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Juárez, A. et al. (2002). Interaction of the Nucleoid-Associated Proteins HAA and H-NS to Modulated Expression of the Hemolysin Operon in Escherichia coli . In: Emoődy, L., Pál, T., Hacker, J., Blum-Oehler, G. (eds) Genes and Proteins Underlying Microbial Urinary Tract Virulence. Advances in Experimental Medicine and Biology, vol 485. Springer, Boston, MA. https://doi.org/10.1007/0-306-46840-9_17
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