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Biomolecules and Nutritional Quality of Soymilk Fermented with Probiotic Yeast and Bacteria

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Abstract

Soymilk was fermented with five isolates of probiotic lactic acid bacteria and in combination with probiotic yeast Saccharomyces boulardii. Nutritional profile like fat, protein, ash, pH, acidity, polyphenol, and protein hydrolysis were analyzed. Polyphenol content decreased from 265.88 to 119 μg/ml with different cultures. Protein hydrolysis ranged from 2.46 to 2.83 mmol l−1 with different cultures. The antioxidant activity was assessed using different methods like 1, 1-diphenyl-2-picrylhydrazyl free radical-scavenging assay, inhibition of ascorbate autoxidation, and measurement of reducing activity. The activities varied with the starters used but, nevertheless, were significantly higher than those found in unfermented soymilk. Bioconversion of the isoflavone glucosides (daidzin + genistin) into their corresponding bioactive aglycones (daidzein + genistein) was observed during soymilk fermentation. Total glucosides in soyamilk were 26.35 mg/100 ml. In contrast, aglycones genistein and daidzein were quantitatively lesser accounting 2.91 mg/100 ml (genistein 1.17 mg/100 ml and daidzein 1.19 mg/100 ml). Soymilk fermented with probiotic cultures resulted in the reduction of glycosides ranging from 0.40 mg to 1.36 mg/100 ml and increase in aglycones ranging from 6.32 mg to 13.66 mg/100 ml.

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Abbreviations

LAB:

lactic acid bacteria

Sb:

Saccharomyces boulardii

La:

Lactobacillus acidophilus

Lb:

Lactobacillus bulgaricus

Lc:

Lactobacillus casei

Lp:

Lactobacillus plantarum

Lh:

Lactobacillus helviticus

References

  1. Dhananjay, S., Kulkarni, S. S., Kapanoor, K. G., Naganagouda, V. K., & Veerappa, H. M. (2006). Biotechnology and Applied Biochemistry, 45, 51–57.

    Article  Google Scholar 

  2. Adlercreutz, H. (2002). Lancer Oncology, 3, 364–373.

    Article  Google Scholar 

  3. Brouns, F. (2002). Food Research International, 35, 187–193.

    Article  CAS  Google Scholar 

  4. Corn well, T., Cohick, W., & Raskin, I. (2004). Phytochemistry, 65, 995–1016.

    Article  CAS  Google Scholar 

  5. Khare, S. K., Krishna, J., & Manishwara, M. N. (1994). Biotechnology and Applied Biochemistry, 19, 193–198.

    CAS  Google Scholar 

  6. Beck, V., Unterrieder, E., Krenn, L., Kubelka, W., & Jungbauer, A. (2003). Journal of Steroid Biochemistry and Molecular Biology, 84, 259–268.

    Article  CAS  Google Scholar 

  7. Setchell, K. D. R. (1998). American Journal of Clinical Nutrition, 68(suppl), 1333S–1346S.

    CAS  Google Scholar 

  8. Setchell, K. D. R., & Cassidy, A. (1999). Journal of Nutrition, 129(suppl), 758S–767S.

    CAS  Google Scholar 

  9. Fukutake, M., Takahashi, M., Ishida, K., Kawamaru, H., Sugimura, T., & Wakabayashi, K. (1996). Food&chemical Toxicology, 34, 457–461.

    Article  CAS  Google Scholar 

  10. Arjmandi, B. H., Birnbaum, R., Goyal, N. V., Getlinger, M. J., Juma, S., & Alekel, L. (1998). American Journal of Clinical Nutrition, 68, 1364S–1368S.

    CAS  Google Scholar 

  11. Potter, S. M., Baum, J. A., Teng, H., Stillman, R. J., Shay, N. F., & Erdman, J. R. (1998). American Journal of Clinical Nutrition, 68, 1375S–1379S.

    CAS  Google Scholar 

  12. Wagner, J. D., Cefalu, W. T., Anthony, M. S., Litwak, K. N., Zhang, L., & Clarkson, T. B. (1997). Metabolism, 46, 698–705.

    Article  CAS  Google Scholar 

  13. King, R. A., & Bignell, C. M. (2000). Aus Journal of Nutrition and Dietetics, 57(2), 70–78.

    Google Scholar 

  14. Chang, Y. C., & Nair, M. G. (1995). Journal of Natural Products, 58, 1892–1896.

    Article  CAS  Google Scholar 

  15. Xu, X., Harris, K. S., Wang, H., Murphy, P. A., & Hendrich, S. (1995). Journal of Nutrition, 125, 2307–2315.

    CAS  Google Scholar 

  16. Fleet, G. H., & Mian, M. A. (1987). International Journal of Food Microbiology, 4, 145–155.

    Article  Google Scholar 

  17. AOAC. (1970). Official methods of analysis (11th ed.). Washington, DC: Association of Official Analytical Chemists.

    Google Scholar 

  18. AOAC. (1984). Official methods of analyis (14th ed.). Washington, DC: Association of Official Analytical Chemists.

    Google Scholar 

  19. Singleton, V. L., & Rossi, J. A. (1965). American Journal of Enology and Viticulture, 16, 144–158.

    CAS  Google Scholar 

  20. Adler-Nissen, J. (1979). Journal of Agricultural and Food Chemistry, 27, 1256–1262.

    Article  CAS  Google Scholar 

  21. Moon, J. H., & Terao, J. (1998). Journal of Agricultural and Food Chemistry, 46, 5062–5065.

    Article  CAS  Google Scholar 

  22. Mishra, O. P., & Kovachich, G. B. (1984). Life Sciences, 35, 849–854.

    Article  CAS  Google Scholar 

  23. Oyaizu, M. (1986). Japanese Journal of Nutrition, 44, 307–315.

    CAS  Google Scholar 

  24. Chiou, R. Y. Y., & Cheng, S. L. (2001). Journal of Agricultural and Food Chemistry, 49, 3656–3660.

    Article  CAS  Google Scholar 

  25. Hou, J-W., Yu, R-C., & Chou, C-C. (2000). Food Research International, 33, 393–397.

    Article  CAS  Google Scholar 

  26. Ankenman Granata, L., & Morr, C. V. (1996). Journal of Food Science, 61(2), 331–336.

    Article  Google Scholar 

  27. Chou, C-C., & Hou, J-W. (2000). International Journal of Food Microbiology, 56, 113–121.

    Article  CAS  Google Scholar 

  28. Karleskind, D., Laye, I., Halpin, E., & Morr, C. V. (1991). Journal of Food Science, 55, 999–1001.

    Article  Google Scholar 

  29. Oberman, H. (1985). CH3. London, England: Applied Science Publishers.

    Google Scholar 

  30. Sindhu, S. C., & Khetarpaul, . (2003). Plants Foods for Human Nutrition, 58, 1–10.

    Article  Google Scholar 

  31. Serraino, M. R., Thompton, L. U., Savoie, L., & Parent, G. (1985). Journal of Food Science, 50, 1689–1692.

    Article  CAS  Google Scholar 

  32. Kurmann, J. A., & Rasic, J. L. (1991). Therapeutic properties of fermented milks pp. 117–158. London: Elsevier Applied Sciences.

    Google Scholar 

  33. Pyo, Y-H., Lee, T-C., & Lee, Y-C. (2005). Food Research International, 38, 551–559.

    Article  CAS  Google Scholar 

  34. Wang, Y-C., Yu, R-C., & Chou, C-C. (2006). Food Microbiology, 23, 128–135.

    Article  Google Scholar 

  35. Chien, H. L. (2004). M.S.Thesis, National Taiwan University, Taipei, Taiwan.

  36. Yang, J. H., Mau, J. L., Ko, P. T., & Huang, L. C. (2000). Antioxidant properties of fermented soybean broth. Food Chemistry, 71, 249–254.

    Article  CAS  Google Scholar 

  37. Tsangalis, D., Ashton, J. E., Magill, A. E. J., & Shah, N. P. (2002). Journal of Food Science, 68, 623–631.

    Article  Google Scholar 

  38. Shimada, K., Fujikawa, K., Yahara, K., & Nakamura, T. (1992). Journal of Agricultural and Food Chemistry, 40, 945–948.

    Article  CAS  Google Scholar 

  39. Izumi, T., Nasu, A., Kataoka, S., Obata, A., & Tobe, K. (2000). Chem Pharma, 1593–1595.

  40. Pyo, Y-H., Lee, T-C., & Lee, Y-C. (2005). Journal of Food Science, 70, S215–S220.

    CAS  Google Scholar 

  41. Laurens-Hatting, A., & Viljoen, B. C. (2001). Food Research International, 34, 791–796.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Food Microbiology, Central Food Technological Research Institute, Mysore, 570020, India

    C. R. Rekha & G. Vijayalakshmi

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  1. C. R. Rekha
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  2. G. Vijayalakshmi
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Correspondence to G. Vijayalakshmi.

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Rekha, C.R., Vijayalakshmi, G. Biomolecules and Nutritional Quality of Soymilk Fermented with Probiotic Yeast and Bacteria. Appl Biochem Biotechnol 151, 452–463 (2008). https://doi.org/10.1007/s12010-008-8213-4

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