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Lipase Production in Solid-State Fermentation Monitoring Biomass Growth of Aspergillus niger Using Digital Image Processing

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Abstract

The aim of this study was to monitor the biomass growth of Aspergillus niger in solid-state fermentation (SSF) for lipase production using digital image processing technique. The strain A. niger 11T53A14 was cultivated in SSF using wheat bran as support, which was enriched with 0.91% (m/v) of ammonium sulfate. The addition of several vegetable oils (castor, soybean, olive, corn, and palm oils) was investigated to enhance lipase production. The maximum lipase activity was obtained using 2% (m/m) castor oil. In these conditions, the growth was evaluated each 24 h for 5 days by the glycosamine content analysis and digital image processing. Lipase activity was also determined. The results indicated that the digital image process technique can be used to monitor biomass growth in a SSF process and to correlate biomass growth and enzyme activity. In addition, the immobilized esterification lipase activity was determined for the butyl oleate synthesis, with and without 50% v/v hexane, resulting in 650 and 120 U/g, respectively. The enzyme was also used for transesterification of soybean oil and ethanol with maximum yield of 2.4%, after 30 min of reaction.

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Acknowledgments

The authors thank Petrobras for the financial support and D.Sc. Denise Maria Guimarães Freire for the lipase immobilization procedures. Lilian Ferreira de Senna and Marta Antunes Pereira Langone thank Prociência Program/UERJ.

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Authors and Affiliations

  1. Departamento de Química Analítica, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier 524, PHLC, IQ, sl 427, Maracanã, Rio de Janeiro, Brazil

    Júlio C. V. Dutra, Marta A. P. Langone & Lilian F. Senna

  2. Embrapa Agroindústria de Alimentos, Avenida das Américas 29501, Rio de Janeiro, Brazil

    Selma da C. Terzi & Sônia Couri

  3. CENPES, PETROBRAS, Av. Jequitiba 950 radial 5 sala 552, Rio de Janeiro, Brazil

    Juliana Vaz Bevilaqua

  4. IT/DTA, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Brazil

    Mônica C. T. Damaso

Authors
  1. Júlio C. V. Dutra
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  2. Selma da C. Terzi
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  3. Juliana Vaz Bevilaqua
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  4. Mônica C. T. Damaso
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  5. Sônia Couri
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  6. Marta A. P. Langone
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  7. Lilian F. Senna
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Correspondence to Sônia Couri.

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Dutra, J.C.V., da C. Terzi, S., Bevilaqua, J.V. et al. Lipase Production in Solid-State Fermentation Monitoring Biomass Growth of Aspergillus niger Using Digital Image Processing. Appl Biochem Biotechnol 147, 63–75 (2008). https://doi.org/10.1007/s12010-007-8068-0

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  • DOI: https://doi.org/10.1007/s12010-007-8068-0

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