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Enzymatic hydrolysis of soybean oil using lipase from different sources to yield concentrated of polyunsaturated fatty acids

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Abstract

The ability of three commercially available lipases to mediate the hydrolysis of the soybean oil to yield concentrated of essential fatty acids was evaluated. The tested lipases were from microbial (Candida rugosa and Thermomyces lanuginosa) and animal cells (Porcine pancreatic lipase). In terms of free fatty acids, microbial lipases were more effective to promote the enzymatic hydrolysis of the soybean oil (over 70%) than the porcine pancreatic lipase (24%). In spite of this, porcine pancreatic lipase (PPL) showed the most satisfactory specificity towards both essential fatty acids and was, therefore, chosen to carry out additional studies. An experimental design was performed taking into consideration the enzyme and NaCl amounts as independent variables. The main effects were fitted by multiple regression analysis to a linear model and maximum fatty acids concentration could be obtained using 3.0 wt% of lipase and 0.08 wt% of NaCl. The mathematical model representing the hydrolysis degree was found to describe adequately the experimental results. Under these conditions, concentrations of 29.5 g/L and 4.6 g/L for linoleic and linolenic acids, respectively, were obtained.

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Abbreviations

EFAs:

Essential fatty acids

PUFAs:

Polyunsaturated fatty acids

FA(s):

Fatty acid(s)

FAMEs:

Fatty acid methyl esters

References

  • AOCS (2004) Official methods and recommended practices of American Oil Chemists’ Society, 5th edn, Second Printing. Am Oil Chem Soc USA, Champaign, Illinois

  • Bauduin P, Renoncourt A, Touraud D, Kunz W, Ninham BW (2004) Hofmeister effect on enzymatic catalysis and colloidal structures. Curr Opin Colloid Interface Sci 9:43–47

    Article  CAS  Google Scholar 

  • Brady L, Brzozowski AM, Derewenda ZS, Dodson E, Dodson G, Tolley S, Turkenburg JP, Christiansen L, Huge-Jensen B, Norskov L, Thim L, Menge U (1990) A serine protease triad forms the catalytic centre of a triacylglycerol lipase. Nature 343:767–770

    Article  CAS  Google Scholar 

  • Brockerhoff H, Jensen RG (1974) Lipolytic enzymes. Academic Press, New York

    Google Scholar 

  • Bueno T (2005) Obtenção de concentrados de ácidos graxos poliinsaturados por hidrólise enzimática do óleo de soja. Brasil. Faculdade de Engenharia Química de Lorena, MSc Dissertation (available on line www.faenquil.br)

  • Carvalho PD, Campos PRB, Noffs MD, Oliveira JG, Shimizu MT, Silva DM (2003) Application of microbial lipases to concentrate polyunsaturated fatty acids. Quim Nova 26:75–80

    CAS  Google Scholar 

  • Ciuffreda P, Loseto A, Manzocchi A, Santaniello E (2001) Lipolytic activity of porcine pancreas lipase on fatty acid esters of dialkylglycerols: a structural basis for the design of new substrates for the assay of pancreatic lipases activity. Chem Phys Lipids 111:105–110

    Article  CAS  Google Scholar 

  • de Castro HF, Mendes AA, Santos JC, Aguiar CL (2004) Modificação de óleos e gorduras por biotransformação. Quim Nova 27:146–156

    Google Scholar 

  • Domínguez de María P, Sánchez-Montero JM (2006) Understanding Candida rugosa lipases: an overview. Biotechnol Adv 24:180–196

    Article  Google Scholar 

  • Faber K (1997) Biotransformation in organic chemistry: a textbook, 3rd edn. Springer Produktions-Gesellschaft, Berlin

    Google Scholar 

  • Ferrato F, Carriere F, Sarda L, Verger R (1997) A critical reevaluation of the phenomenon of interfacial activation. In: Rubin B, Dennis FA (eds) Methods in enzymology, vol 286. Academic Press, New York, pp 327–347

    Google Scholar 

  • Fuentes G, Ballesteros A, Verma CS (2004) Specificity in lipases: a computational study of transesterification of sucrose. Protein Sci 13:3092–3103

    Article  CAS  Google Scholar 

  • Haraldsson GG (1991) The applications of lipases for modification of fats and oils, including marine oils. Mar Lipids Biotechnol 7:337–352

    Google Scholar 

  • Kuen ST (2001) An overview of the Asean olechemical market. Malasyan Oil Sci Technol 10:59–71

    Google Scholar 

  • Mendes AA, Castro HF (2005) Effect on the enzymatic hydrolysis of lipids from dairy wastewater by replacing gum arabic emulsifier for sodium chloride. Braz Arch Biol Techn 48:135–142

    Google Scholar 

  • Noel M, Combes D (2003) Rhizomucor miehei lipase: differential scanning calorimetry and pressure/temperature stability studies in presence of soluble additives. Enzyme Microb Tech 33:299–308

    Article  CAS  Google Scholar 

  • Pleiss J, Fischer M, Schmid RD (1998) Anatomy of lipase binding sites: the scissile fatty acid binding site. Chem Phys Lipids 93:67–80

    Article  CAS  Google Scholar 

  • Rooney D, Weatherley LR (2001) The effect of reaction conditions upon lipase catalysed hydrolysis of high oleate sunflower oil in a stirred liquid-liquid reactor. Process Biochem 36:947–953

    Article  CAS  Google Scholar 

  • Salis A, Svensson I, Monduzzi M, Solinas V, Adlercreutz P (2003) The atypical lipase B from Candida antarctica is better adapted for organic media than the typical lipase from Thermomyces lanuginosa. Biochim Biophys Acta 1646:145–151

    CAS  Google Scholar 

  • Schmid RD, Verger R (1998) Lipases: interfacial enzymes with attractive applications. Angew Chem Int Edit 37:1609–1633

    CAS  Google Scholar 

  • Sonntag NO (1979) Structure and composition of fats and oils. In: Swern D (eds) Bailey`s industrial oil and fat products, 4th edn, vol 1. John Wiley & Sons Inc., New Jersey

    Google Scholar 

  • Vergroesen AJ (1976) Early signs of polyunsaturated fatty acid deficiency. Biblthca Nutr Dieta 23:19–25

    Google Scholar 

  • Ward OP, Singh A (2005) Omega-3/6 fatty acids: alternative sources of production. Process Biochem 40:3627–3652

    Article  CAS  Google Scholar 

  • Willis WM, Lencki RW, Marangoni AG (1998) Lipid modification strategies in the production of nutritionally functional fats and oils. Cr Rev Food Sci Nut 38:639–674

    Article  CAS  Google Scholar 

  • Winkler FK, d’Arcy A, Hunziker W (1990) Structure of human pancreatic lipase. Nature 343:771–774

    Article  CAS  Google Scholar 

  • Wu XY, Jääskelänen S, Linko Y (1996) An investigation of crude lipases for hydrolysis, esterification, and transesterification. Enzyme Microb Technol 19:226–231

    Article  CAS  Google Scholar 

  • Yang Y, Lowe ME (2000) The open lid mediates pancreatic lipase function. J Lipids Res 41:48–57

    CAS  Google Scholar 

Download references

Acknowledgments

The authors acknowledge the financial assistance from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Correspondence to Heizir Ferreira de Castro.

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Freitas, L., Bueno, T., Perez, V.H. et al. Enzymatic hydrolysis of soybean oil using lipase from different sources to yield concentrated of polyunsaturated fatty acids. World J Microbiol Biotechnol 23, 1725–1731 (2007). https://doi.org/10.1007/s11274-007-9421-8

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