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High conversion frequency of germinated somatic embryos of Siberian ginseng (Eleutherococcus senticosus Maxim) using a bubble column bioreactor

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Abstract

Embryogenic callus was obtained from young petiole, stem, and root explants of 4-year-old Siberian ginseng plants on medium supplemented with 2,4-dichlorophenoxyacetic acid. Embryogenic callus differentiated into somatic embryos (SEs), most of which could germinate but developed abnormally. Friable embryogenic callus was induced mainly from the root regions of germinated primary SEs or regenerated plantlets on plant growth regulator-free medium. Histological studies showed that the embryogenic callus initiated from the subepidermal cells of young roots. The bioreactor system was more efficient than suspension culture regarding the number and growth of SEs, although a similar amount of embryonic tissue was used. An average of 115,370 germinated SEs developed from an initial 400 mg of embryogenic callus, and 64.7 % of germinated SEs converted into plantlets after a 4-weeks culture on agar medium. During the bioreactor culture process, secondary SEs were induced directly from SEs at various stages, a phenomenon that rarely occurred in suspension culture. These secondary SEs developed quickly and germinated during the bioreactor culture process. Proline content and peroxidase and catalase activities of SEs cultured in bioreactors were higher than in SEs cultured in suspension culture.

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Acknowledgments

This work was supported by the 948 Project of State Forestry Administration of China (2009-4-26), National Natural Science Foundation of China (No. 30671701), the excellent doctor degree dissertation program in Northeast Forestry University (OPTP10-NEFU), the program for Changjiang Scholars and Innovative Research Team in University, and the Fundamental Research Funds for the Central Universities (DL11EA02).

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Authors and Affiliations

  1. State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China

    Jingli Yang, Chenguang Zhou, Lihui Liu, Du Jia, Zhiyang Yin & Chenghao Li

  2. Division of Bioresource Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, 200-701, South Korea

    Myongjo Kim & Changyeon Yu

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  1. Jingli Yang
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  2. Chenguang Zhou
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  3. Lihui Liu
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  4. Du Jia
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  5. Zhiyang Yin
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  6. Myongjo Kim
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  7. Changyeon Yu
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  8. Chenghao Li
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Correspondence to Changyeon Yu or Chenghao Li.

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Yang, J., Zhou, C., Liu, L. et al. High conversion frequency of germinated somatic embryos of Siberian ginseng (Eleutherococcus senticosus Maxim) using a bubble column bioreactor. Plant Cell Tiss Organ Cult 110, 289–298 (2012). https://doi.org/10.1007/s11240-012-0150-9

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  • DOI: https://doi.org/10.1007/s11240-012-0150-9

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