Skip to main content

Advertisement

SpringerLink
Log in

Cellularity and apparent diffusion coefficient in oligodendroglial tumours characterized by genotype

  • Clinical Study - Patient Study
  • Published:
Journal of Neuro-Oncology Aims and scope Submit manuscript

Abstract

Purpose Apparent diffusion coefficient (ADC) describes water diffusion within tissues. Previous studies report a negative linear correlation between minimum ADC and tumour cellularity in different types of gliomas, but there are no studies in oligodendroglial tumours. This study evaluated the relationship between ADC and tumour cellularity in oligodendroglial tumours characterized by genotype. Methods ADC was assessed in 17 patients with known 1p/19q status: 3 grade II oligodendrogliomas (OII), 9 grade II oligoastrocytomas (OAII), 5 grade III oligoastrocytomas (OAIII). Regions of interest were placed on ADC maps around tumour margins to generate mean tumour ADC, and over minimum and maximum tumour ADC. Histopathology assessment of tumour cellularity determined minimum, maximum and mean cell density in serial stereotactic biopsies. Results 1p/19q loss was present in 2/3 OII, 5/9 OAII, 2/5 OAIII. Grade III tumours had higher maximum cell density than grade II tumours (17.2 vs. 10.57%: Mann Whitney U; P = 0.20). Oligoastrocytoma were more likely to have a lower minimum cell density than oligodendrogliomas (Mann Whitney U; P = 0.032). There was no relationship between cell density and genotype. There was no linear correlation between mean ADC and mean cell density (Spearman’s rho; r = 0.486: P = 0.438), minimum ADC and maximum cell density (Spearman’s rho; r = 0.158: P = 0.660), and maximum ADC and minimum cell density (Spearman’s rho; r = 0.039: P = 0.985). Conclusions In oligodendroglial tumours there is no relationship between quantitative assessment of cellularity and ADC. This may reflect differences in oligodendroglial tumour biology compared to other gliomas, although the composition of the extracellular matrix may influence ADC more than cellularity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Le Bihan D, Poupon C, Amadon A, Lethimonnier F (2006) Artifacts and pitfalls in diffusion MRI. J Magn Reson Imaging 24:478–488

    Article  PubMed  Google Scholar 

  2. Gupta RK, Cloughesy TF, Sinha U, Garakian J, Lazareff J, Rubino G, Rubino L, Becker DP, Vinters HV, Alger JR (2000) Relationships between choline magnetic resonance spectroscopy, apparent diffusion coefficient and quantitative histopathology in human glioma. J Neurooncol 50:215–226

    Article  CAS  PubMed  Google Scholar 

  3. Sugahara T, Korogi Y, Kochi M, Ikushima I, Shigematu Y, Hirai T, Okuda T, Liang L, Ge Y, Komohara Y, Ushio Y, Takahashi M (1999) Usefulness of diffusion-weighted MRI with echo-planar technique in the evaluation of cellularity in gliomas. J Magn Reson Imaging 9:53–60

    Article  CAS  PubMed  Google Scholar 

  4. Kono K, Inoue Y, Nakayama K, Shakudo M, Morino M, Ohata K, Wakasa K, Yamada R (2001) The role of diffusion-weighted imaging in patients with brain tumors. AJNR Am J Neuroradiol 22:1081–1088

    CAS  PubMed  Google Scholar 

  5. Chiang IC, Kuo YT, Lu CY, Yeung KW, Lin WC, Sheu FO, Liu GC (2004) Distinction between high-grade gliomas and solitary metastases using peritumoral 3-T magnetic resonance spectroscopy, diffusion, and perfusion imagings. Neuroradiology 46:619–627

    Article  PubMed  Google Scholar 

  6. Yang D, Korogi Y, Sugahara T, Kitajima M, Shigematsu Y, Liang L, Ushio Y, Takahashi M (2002) Cerebral gliomas: prospective comparison of multivoxel 2D chemical-shift imaging proton MR spectroscopy, echoplanar perfusion and diffusion-weighted MRI. Neuroradiology 44:656–666

    Article  CAS  PubMed  Google Scholar 

  7. Chenevert TL, Stegman LD, Taylor JM, Robertson PL, Greenberg HS, Rehemtulla A, Ross BD (2000) Diffusion magnetic resonance imaging: an early surrogate marker of therapeutic efficacy in brain tumors. J Natl Cancer Inst 92:2029–2036

    Article  CAS  PubMed  Google Scholar 

  8. Hein PA, Eskey CJ, Dunn JF, Hug EB (2004) Diffusion-weighted imaging in the follow-up of treated high-grade gliomas: tumor recurrence versus radiation injury. AJNR Am J Neuroradiol 25:201–209

    PubMed  Google Scholar 

  9. Jager HR, Waldman AD, Benton C, Fox N, Rees J (2005) Differential chemosensitivity of tumor components in a malignant oligodendroglioma: assessment with diffusion-weighted, perfusion-weighted, and serial volumetric MR imaging. AJNR Am J Neuroradiol 26:274–278

    PubMed  Google Scholar 

  10. Tozer DJ, Jager HR, Danchaivijitr N, Benton CE, Tofts PS, Rees JH, Waldman AD (2007) Apparent diffusion coefficient histograms may predict low-grade glioma subtype. NMR Biomed 20:49–57

    Article  PubMed  Google Scholar 

  11. Megyesi JF, Kachur E, Lee DH, Zlatescu MC, Betensky RA, Forsyth PA, Okada Y, Sasaki H, Mizoguchi M, Louis DN, Cairncross JG (2004) Imaging correlates of molecular signatures in oligodendrogliomas. Clin Cancer Res 10:4303–4306

    Article  PubMed  Google Scholar 

  12. Jenkinson MD, du Plessis DG, Smith TS, Joyce KA, Warnke PC, Walker C (2006) Histological growth patterns and genotype in oligodendroglial tumours: correlation with MRI features. Brain 129:1884–1891

    Article  PubMed  Google Scholar 

  13. Jenkinson MD, Smith TS, Joyce KA, Fildes D, Broome J, du Plessis DG, Haylock B, Husband DJ, Warnke PC, Walker C (2006) Cerebral blood volume, genotype and chemosensitivity in oligodendroglial tumours. Neuroradiology 48:703–713

    Article  PubMed  Google Scholar 

  14. Jenkinson MD, Smith TS, Brodbelt AR, Joyce KA, Warnke PC, Walker C (2007) Apparent diffusion coefficients in oligodendroglial tumours characterised by genotype. J Magn Reson Imaging 26:1405–1412

    Article  PubMed  Google Scholar 

  15. Walker C, du Plessis DG, Fildes D, Haylock B, Husband D, Jenkinson MD, Joyce KA, Broome J, Kopitski K, Prosser J, Smith T, Vinjamuri S, Warnke PC (2004) Correlation of molecular genetics with molecular and morphological imaging in gliomas with an oligodendroglial component. Clin Cancer Res 10:7182–7191

    Article  CAS  PubMed  Google Scholar 

  16. Walker C, du Plessis DG, Joyce KA, Fildes D, Gee A, Haylock B, Husband D, Smith T, Broome J, Warnke PC (2005) Molecular pathology and clinical characteristics of oligodendroglial neoplasms. Ann Neurol 57:855–865

    Article  CAS  PubMed  Google Scholar 

  17. Walker C, Haylock B, Husband D, Joyce KA, Fildes D, Jenkinson MD, Smith T, Broome J, du Plessis DG, Warnke PC (2006) Clinical use of genotype to predict chemosensitivity in oligodendroglial tumors. Neurology 66:1661–1667

    Article  CAS  PubMed  Google Scholar 

  18. Walker C, Haylock B, Husband D, Joyce KA, Fildes D, Jenkinson MD, Smith T, Broome J, Kopitzki K, du Plessis DG, Prosser J, Vinjamuri S, Warnke PC (2006) Genetic and metabolic predictors of chemosensitivity in oligodendroglial neoplasms. Br J Cancer 95:1424–1431

    Article  CAS  PubMed  Google Scholar 

  19. Louis DN, Ohgaki H, Wiestler OD, Cavanee WK (2007) WHO classification of tumours of the central nervous system. IARC Press, Lyon

  20. Le Bihan D, Breton E, Lallemand D, Grenier P, Cabanis E, Laval-Jeantet M (1986) MR imaging of intravoxel incoherent motions: application to diffusion and perfusion in neurologic disorders. Radiology 161:401–407

    CAS  PubMed  Google Scholar 

  21. Gladson CL (1999) The extracellular matrix of gliomas: modulation of cell function. J Neuropathol Exp Neurol 58:1029–1040

    Article  CAS  PubMed  Google Scholar 

  22. Sadeghi N, Camby I, Goldman S, Gabius HJ, Baleriaux D, Salmon I, Decaesteckere C, Kiss R, Metens T (2003) Effect of hydrophilic components of the extracellular matrix on quantifiable diffusion-weighted imaging of human gliomas: preliminary results of correlating apparent diffusion coefficient values and hyaluronan expression level. AJR Am J Roentgenol 181:235–241

    PubMed  Google Scholar 

  23. Beppu T, Inoue T, Shibata Y, Yamada N, Kurose A, Ogasawara K, Ogawa A, Kabasawa H (2005) Fractional anisotropy value by diffusion tensor magnetic resonance imaging as a predictor of cell density and proliferation activity of glioblastomas. Surg Neurol 63:56–61 (discussion 61)

    Article  PubMed  Google Scholar 

  24. Price SJ, Jena R, Burnet NG, Hutchinson PJ, Dean AF, Pena A, Pickard JD, Carpenter TA, Gillard JH (2006) Improved delineation of glioma margins and regions of infiltration with the use of diffusion tensor imaging: an image-guided biopsy study. AJNR Am J Neuroradiol 27:1969–1974

    CAS  PubMed  Google Scholar 

  25. Stadlbauer A, Ganslandt O, Buslei R, Hammen T, Gruber S, Moser E, Buchfelder M, Salomonowitz E, Nimsky C (2006) Gliomas: histopathologic evaluation of changes in directionality and magnitude of water diffusion at diffusion-tensor MR imaging. Radiology 240:803–810

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

Supported by grants from The Walton Centre Neuroscience Fund, Clatterbridge Cancer Research Trust and The Royal Colleges of Edinburgh and Ireland.

Conflict of interest statement

We declare that we have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Neurosurgery, The Walton Centre for Neurology and Neurosurgery, Lower Lane, Liverpool, L9 7LJ, UK

    Michael D. Jenkinson & Andrew R. Brodbelt

  2. Department of Neuroradiology, The Walton Centre for Neurology and Neurosurgery, Liverpool, L9 7LJ, UK

    Trevor S. Smith

  3. Department of Neuropathology, Hope Hospital, Manchester, M6 8HD, UK

    Daniel G. du Plessis

  4. School of Cancer Studies, University of Liverpool, Liverpool, L3 9TA, UK

    Michael D. Jenkinson, Kathy A. Joyce & Carol Walker

Authors
  1. Michael D. Jenkinson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Daniel G. du Plessis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Trevor S. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Andrew R. Brodbelt
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kathy A. Joyce
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Carol Walker
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Michael D. Jenkinson.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jenkinson, M.D., du Plessis, D.G., Smith, T.S. et al. Cellularity and apparent diffusion coefficient in oligodendroglial tumours characterized by genotype. J Neurooncol 96, 385–392 (2010). https://doi.org/10.1007/s11060-009-9970-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11060-009-9970-9

Keywords

Search

Navigation