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Heavy-ion beam irradiation is an effective technique for reducing major allergens in peanut seeds

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Abstract

Heavy-ion beam irradiation is an effective technique for mutation breeding to produce new cultivars. Heavy-ion beams have high linear energy transfer capable of breaking the double-stranded DNA molecules, thus inducing stable knockout mutants. Here, we report the first application of this technology to produce hypoallergenic peanut lacking major allergens, Ara h 2 and 3, from the Japanese Nakateyutaka variety. After irradiation with either N or C heavy-ion beams at a dose of 100 Gy, seventeen knockout mutants from 11,335 screened M2 seeds were obtained, eight of which lacked either one of the two isoforms of Ara h 2, and the other nine lacked one of the isoforms of Ara h 3. This result indicates that heavy-ion beam irradiation is a powerful means of producing knockout hypoallergenic peanuts.

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Abbreviations

HIBI:

Heavy-ion beam irradiation

RIBF:

RIKEN Nishina Center Radioactive-Isotope Beam Factory

IUIS:

International Union of Immunological Societies

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

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Acknowledgments

We thank Prof. Reiko Urade (Kyoto University) for warm encouragement. The first author also wishes to thank the Japanese Government (Monbukagakusho:MEXT) for scholarship support.

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Authors and Affiliations

  1. Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 611-0011, Japan

    Cerrone S. Cabanos, Hiroki Katayama, Hiroyuki Urabe & Nobuyuki Maruyama

  2. Chiba Prefectural Agriculture and Forestry Research Center, Upload Crops Breeding Laboratory, Chiba, Japan

    Chikara Kuwata

  3. Chiba Prefectural Agriculture and Forestry Research Center, Biotechnology Laboratory, Chiba, Japan

    Yuri Murota

  4. Radiation Biology Team, RIKEN Nishina Center, RIKEN, Hirosawa, Wako, 351-0198, Japan

    Tomoko Abe

  5. Laboratory of Plant Breeding, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan

    Yutaka Okumoto

Authors
  1. Cerrone S. Cabanos
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  2. Hiroki Katayama
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  3. Hiroyuki Urabe
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  4. Chikara Kuwata
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  6. Tomoko Abe
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  8. Nobuyuki Maruyama
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Correspondence to Nobuyuki Maruyama.

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Cabanos, C.S., Katayama, H., Urabe, H. et al. Heavy-ion beam irradiation is an effective technique for reducing major allergens in peanut seeds. Mol Breeding 30, 1037–1044 (2012). https://doi.org/10.1007/s11032-011-9687-2

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  • DOI: https://doi.org/10.1007/s11032-011-9687-2

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