Abstract
Reperfusion injury causes oxidative stress thereby resulting in an imbalance between oxidant-antioxidant systems. In the present communication, the effect of ascorbic acid supplementation has been studied on certain oxidant and antioxidant parameters in the blood of the patients with myocardial infarction before and after thrombolysis. In patients after thrombolysis, the activity of antioxidant enzyme, superoxide dismutase, in the blood was found to be significantly reduced where as the activity of the oxidant enzyme, xanthine oxidase, was found to be significantly increased. Malondialdehyde levels, the index of free radical mediated damage, was also found to be significantly elevated in thrombolyzed patients compared to the patients before thrombolysis. Supplementation of vitamin C to the post reperfusion patients restored these parameters back to normal or near normal levels.
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References
Frei B, England L, Ames BN: Ascorbate is an outstanding antioxidant in human blood plasma. Proc Natl Acad Sci USA 86: 6377–6381, 1989
Frei B, Stocker R, England L, Ames BN: Ascorbate: the most effective antioxidant in human blood plasma. Adv Exp Med Biol 264: 155–163, 1990
Weber P, Bendich A, Schalch W: Vit C and human health-a review of recent data related to human requirements. Int J Vitam Nutr Res 66: 19–30, 1996
Enstrom JE: Vit C in prospective epidemiological studies. In: L Packer, J Fuchs (eds) Vitamin C in health and disease. New York: Marcel Decker Inc. 381–398, 1997
Gey KF: Vitamins E plus C and interacting conutrients required for optimal health. Biofactors 7: 113–174, 1998
Bordia AK: The effect of Vitamin C on blood lipids, Fibrinolytic activity and platelet adhesiveness in patients with coronary artery disease. Atherosclerosis 35: 181–187, 1980
Bordia A, Verma SK: Effect of vitamin C on platelet adhesiveness and platelet aggregation in coronary artery disease patients. Clin Cardiol 8: 552–554, 1985
Halliwell B: Vitamin C: antioxidant or pro-oxidant in Vivo. Free Radic Res 25: 439–454, 1996
Packer L: Vitamin C and redox cycling antioxidants. In: L Packer, J Fuchs, (eds). Vitamin C in health and disease. New York: Marcel Dekker Inc 95–121, 1997
Bowry VW, Mohr D, Cleary J, Stocker R: Prevention of tocopherol mediated peroxidation in ubiquinol-10-free human low density lipoprotein. J Biol Chem 270: 5756–5763, 1995
Klenner FR: Observations on the dose and observations of ascorbic acid when employed beyond the range of a vitamin in human pathology. J App Nutr 23: 61–88, 1971
Cathcart RF: Vitamin C: titrating to bowel tolerance, an ascorbemia and acute induced scurvy. Medical Hypothesis 7: 1359–1376, 1981
Tsao CS: An overview of ascorbic acid chemistry and biochemistry. In: Packer L, Fuchs J, (eds). Vitamin C in health and Disease. New York: Marcel Dekker Inc. 25–58, 1997
McCord JM: Oxygen free radicals in post ischemic tissue injury. N Engl J Med 312: 159–163, 1985
Halliwell B: Oxygen free radicals and metal ions: Potentail antioxidant intervention strategies. In Cress CE (moderator): Oxygen free radicals and human disease. Ann Int Med 107: 526–545, 1987
Halliwell B, Gutterridge GMC, Cross CE: Free radicals, antioxidants and human disease : where are we now? J Lab Clin Invest 119: 589–620, 1992
Slater TF, Cheesman KH, Davies MJ, Proudfoot K, Xin W: Free radical mechanisms in relation to tissue injury. Proc Nutr Soc 46: 1–12, 1987
Warso MA, Lands EM: Presence of lipid hydroperoxide in human blood plasma. J Clin Invest 75: 667–671, 1985
Frei B, Stocker R, Ames BN: Antioxidant defenses and lipid peroxidation in human blood plasma. Proc Natl Acad Sci USA 85: 9748–9752, 1988
Levy Y, Ben-Amotz A, Aviram M: Effect of dietry supplemention of different β-carotene isomers on lipoprotein oxidative modification. J Nutr Med 5: 13–22, 1995
Goldhaber J, Weiss JN: Oxygen free radicals and cardiac reperfusion abnormalities. Hypertension 20: 118–127, 1992
Ambroso G, Chiarello M: Myocardial reperfusion injury: mechanisms and management-a review. Am J Med 91: 86–88, 1991
Ferrari R, Ceconi C, Curello S, Cargnoni A, De Guili F, Visioli O: Occurrence of oxidative stress during myocardial reperfusion. Mol Cell Biochem 111: 61–69, 1992
Luchesi BR: Myocardial Ischemia-reperfusion and free radical injury. Am J Cardiol 65: 141–231, 1990.
Misra HP, Fridovich I: The role of superoxide anion in the auto oxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247: 3170–3175, 1972
Roussos GG: Xanthine oxidase from bovine small intestine. Meth Enzymol 12: 5–16, 1967
Lowry OH, Rosebrough NJ, Farr AL, Randall RG: Protein measurement with Folin phenol reagent. J Biol Chem 193: 265–275, 1951
Hu M: Measurement of protein thiol groups and glutathione in plasma. Methods Enzymol 233: 380–382, 1994
Ohkawa H, Oshishi N, Yagi K: Assay of lipid peroxidation in animal tissues by thiobarbituric acid reaction. Anal Biochem 95: 351–358, 1979
Maxwell SRJ, Lip GYH. Reperfusion injury: a review of the pathophysiology, clinical manifestation and therapeutic options. Int J Cardiol 58: 95–117, 1997
Zweir JL, Flaherty JT, Weisfeldt ML: Direct measurement of free radical generation following reperfusion of ischemic myocardium. Proc Natl Acad Sci USA 84: 1404–1407, 1987
Bolli R: Oxygen-derived free radicals and myocardial reperfusion injury: an overview. Cardiovasc Drug Ther 5: 249–268, 1991
Bhakuni P, Chandra M, Misra MK: Levels of free radicals scavengers and antioxidants in post-reperfused patients of myocardial infarction. Current Sci 89: 168–170, 2005
Robert MJD, Young IS, Trouton TG, Trimble ER, Khan MM, Webb SW, Wilson CM, Patterson GC, Adgey AA: Transient release of lipid peroxides after coronary artery balloon angioplasty. Lancet 336: 143–145, 1990
Coughlan JG, Flitter WD, Holley AE, Norell M, Mitchell AG, Iisley CD, Slater TF: Detection of free radicals and cholesterol hydroperoxide in blood taken from the coronary sinus of man during percutaneous transluminal coronary angioplasty. Free Rad Res Comm 14: 403–413, 1991
Davies SW, Ranjadayalan K, Wickens DG, Dormandy TL, Timmis AD: Lipid peroxidation associated with successful thrombolysis. Lancet 335: 741–743, 1990
Ekelund UEG, Harrison RW, Shokek O, Thakkar RN, Tunin RS, Senzaki H, Kass DA, Marban E, Hare JM: Intravenous allopurinol decreases myocardial oxygen consumption and increases mechanical efficiency in dogs with pacing-induced heart failure. Circ Res 85: 437–445, 1999
Cappola TP, Kass DA, Nelson GS, Berger RD, Rosas GO, Kobeissi ZA, Marban E, Hare JM: Allopurinol improves myocardial efficiency in patients with idiopathic dilated cardiomyopathy. Circulation 104: 2407–2411, 2001
Jialal I, Gundy SN: Preservation of the endogenous antioxidants in low density lipoprotein by ascorbate but not probucol during oxidative modification. J Clin Invest 86: 597–601, 1991
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Bhakuni, P., Chandra, M. & Misra, M.K. Effect of Ascorbic Acid Supplementation on Certain Oxidative Stress Parameters in the Post Reperfusion Patients of Myocardial Infarction. Mol Cell Biochem 290, 153–158 (2006). https://doi.org/10.1007/s11010-006-9182-y
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DOI: https://doi.org/10.1007/s11010-006-9182-y