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Diesel Exhaust Particle-Exposed Human Bronchial Epithelial Cells Induce Dendritic Cell Maturation and Polarization via Thymic Stromal Lymphopoietin

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Abstract

Human exposure to air pollutants, including ambient particulate matter, has been proposed as a mechanism for the rise in allergic disorders. Diesel exhaust particles, a major component of ambient particulate matter, induce sensitization to neoallergens, but the mechanisms by which sensitization occur remain unclear. We show that diesel exhaust particles upregulate thymic stromal lymphopoietin in human bronchial epithelial cells in an oxidant-dependent manner. Thymic stromal lymphopoietin induced by diesel exhaust particles was associated with maturation of myeloid dendritic cells, which was blocked by anti-thymic stromal lymphopoietin antibodies or silencing epithelial cell-derived thymic stromal lymphopoietin. Dendritic cells exposed to diesel exhaust particle-treated human bronchial epithelial cells induced Th2 polarization in a thymic stromal lymphopoietin-dependent manner. These findings provide new insight into the mechanisms by which diesel exhaust particles modify human lung mucosal immunity.

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Acknowledgements

This work was supported by National Institutes of Health Grants ES010187 (JR), T32 ES007267 (JR), MO1RR00096, AI27742-129007 (DT) and AI44729 (DT). We thank Rashedul Ahsan for his technical support for ROS studies.

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Authors and Affiliations

  1. Department of Medicine, Division of Pulmonary and Critical Care Medicine, New York University School of Medicine, 550 1st Avenue, Room NB7N24, New York, NY, USA

    Bertram Bleck, Doris B. Tse, Feijie Zhang & Joan Reibman

  2. Division of Infectious Diseases, Department of Medicine, New York University School of Medicine, New York, NY, USA

    Doris B. Tse

  3. Department of Pathology, Molecular Pathogenesis Program, New York University School of Medicine, New York, NY, USA

    Maria A. Curotto de Lafaille

  4. Skirball Institute for Biomolecular Medicine, New York University School of Medicine, New York, NY, USA

    Maria A. Curotto de Lafaille

  5. Department of Medicine and Environmental Medicine, New York University School of Medicine, New York, NY, USA

    Joan Reibman

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  1. Bertram Bleck
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  2. Doris B. Tse
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  3. Maria A. Curotto de Lafaille
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  4. Feijie Zhang
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  5. Joan Reibman
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Correspondence to Joan Reibman.

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Bleck, B., Tse, D.B., Curotto de Lafaille, M.A. et al. Diesel Exhaust Particle-Exposed Human Bronchial Epithelial Cells Induce Dendritic Cell Maturation and Polarization via Thymic Stromal Lymphopoietin. J Clin Immunol 28, 147–156 (2008). https://doi.org/10.1007/s10875-007-9149-0

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  • DOI: https://doi.org/10.1007/s10875-007-9149-0

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