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Salinity-induced changes in two cultivars of Vigna radiata: responses of antioxidative and proline metabolism

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Abstract

The influence of increasing salinity stress on plant growth, antioxidant enzymes and proline metabolism in two cultivars of Vigna radiata L. (cv. Pusa Bold and cv. CO 4) was investigated. Salt stress was imposed on 30-days-old cultivars with four different concentrations of NaCl (0, 100, 200 and 300 mM). The roots and shoots of CO 4 showed greater reduction in fresh weight, dry weight and water content when compared to Pusa Bold with increasing salt stress. Under salinity stress, the roots and shoots of CO 4 exhibited higher Na+: K+ ratio than Pusa Bold. The activities of reactive oxygen species (ROS) scavenging enzymes and reduced glutathione (GSH) concentration were found to be higher in the leaves of Pusa Bold than in CO 4, whereas oxidized glutathione (GSSG) concentration was found to be higher in the leaves of CO 4 compared to those in Pusa Bold. Our studies on oxidative damage in two Vigna cultivars showed lower levels of lipid peroxidation and H2O2 concentration in Pusa Bold than in CO 4 under salt stress conditions. High accumulation of proline and glycine betaine under salt stress was also observed in Pusa Bold when compared to CO 4. The activities of proline biosynthetic enzymes were significantly high in Pusa Bold. However, under salinity stress, Pusa Bold showed a greater decline in proline dehydrogenase (ProDH) activity compared to CO 4. Our data in this investigation demonstrate that oxidative stress plays a major role in salt-stressed Vigna cultivars and Pusa Bold has efficient antioxidative characteristics which could provide better protection against oxidative damage in leaves under salt-stressed conditions.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

GSH:

Reduced glutathione

GSSG:

Oxidized glutathione

GR:

Glutathione reductase

MDAR:

Monodehydroascorbate reductase

POD:

Peroxidase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

TBA:

Thiobarbituric acid

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Acknowledgements

Thanks are due to Professor A. R. Muthaiah, Center for Plant Breeding and Genetics, Tamilnadu Agricultural University (TNAU), Coimbatore, India for providing the Vigna cultivars. Research Fellowships to KS and PPJ from Jawaharlal Nehru Memorial Fund (JNMF) are gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry, 605 014, India

    K. Sumithra & B. Dalton Carmel

  2. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500 046, India

    P. P. Jutur & Attipalli R. Reddy

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  1. K. Sumithra
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  2. P. P. Jutur
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  3. B. Dalton Carmel
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  4. Attipalli R. Reddy
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Correspondence to Attipalli R. Reddy.

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Sumithra, K., Jutur, P.P., Carmel, B.D. et al. Salinity-induced changes in two cultivars of Vigna radiata: responses of antioxidative and proline metabolism. Plant Growth Regul 50, 11–22 (2006). https://doi.org/10.1007/s10725-006-9121-7

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  • DOI: https://doi.org/10.1007/s10725-006-9121-7

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