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Reduction of pollutants in pulp paper mill effluent treated by PCP-degrading bacterial strains

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Abstract

Two PCP-degrading bacterial strains, Bacillus cereus (ITRC-S6) and Serratia marcescens (ITRC-S7) were used for the treatment of pulp and paper mill effluent at conditions; 1.0% glucose and 0.5% peptone at 30 ± 1°C at 120 rpm for 168 h of incubation. These two bacterial strains effectively reduced colour (45–52%), lignin (30–42%), BOD (40–70%), COD (50–60%), total phenol (32–40%) and PCP (85–90%) within 168 h of incubation. However, the highest reduction in colour (62%), lignin (54%), BOD (70%), COD (90%), total phenol (90%) and PCP (100%) was recorded by mixed culture treatment. The bacterial mechanism for the degradation of pulp and paper mill effluent may be explained by an increase in the cells biomass using added co-substrates resulting liberation of significant amount of chloride due to bacterial dechlorination of chlorolignins and chlorophenols this showed reduction in colour, lignin and toxicity in the effluent. Further, GC-MS analysis of ethyl acetate-extractable compounds from treated pulp paper mill effluent reinforces the bacterium capability for the degradation of lignin and pentachlorophenol, as many aromatic compounds such as 2-chlorophenol, 2, 4, 6-trichlorophenol and tetrachlorohydroquinone, 6-chlorohydroxyquinol and tetrachlorohydroquinone detected which were not present in the untreated effluent.

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  1. Environmental Microbiology Section, Industrial Toxicology Research Centre, Post Box No. 80, M.G. Marg, Lucknow, UP, 226001, India

    Ram Chandra, Abhay Raj & Sangeeta Yadav

  2. Analytical Chemistry Division, Industrial Toxicology Research Centre, Post Box No. 80, M.G. Marg, Lucknow, UP, 226001, India

    Devendra Kumar Patel

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  1. Ram Chandra
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  2. Abhay Raj
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Chandra, R., Raj, A., Yadav, S. et al. Reduction of pollutants in pulp paper mill effluent treated by PCP-degrading bacterial strains. Environ Monit Assess 155, 1–11 (2009). https://doi.org/10.1007/s10661-008-0413-4

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