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Therapeutic Effect of Repeated Natural Killer T Cell Stimulation in Mouse Cholangitis Complicated by Colitis

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Abstract

Primary sclerosing cholangitis is often complicated by ulcerative colitis. Recently, we reported on Th1-dominant cholangitis associated with experimental colitis, and natural killer T (NKT) cells might play an important role in this model. The aim of this study was to clarify the immunopathogenic role of NKT cells in this model using α-galactosylceramide. CD-1 mice were administered 2.0% dextran sulfate sodium for 29 days and injection of α-galactosylceramide was performed every 5 days, then inflammation was assessed. Mononuclear cells from the liver were analyzed with respect to cytokine production and the surface marker. α -Galactosylceramide improved survival rate, weight gain, and inflammation score. Also, interferon-γ release from MNC, CD4/CD8 ratio, NKT cell population, and NK cell population were decreased by this treatment. These findings indicate that repeated stimulation of NKT cells modifies the Th1/Th2 balance to reduce Th1 dominance, and this may be a mechanism by which α -galactosylceramide has a therapeutic effect.

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Authors and Affiliations

  1. Department of General Medicine and Clinical Pharmacotherapy, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan

    Yoshihiro Numata, Susuma Tazuma Md, Phd, Yoshitaka Ueno, Tomoji Nishioka Md, Phd, Hideyuki Hyogo Md, Phd & Kazuaki Chayama Md, Phd

  2. Department of General Medicine and Clinical Pharmacotherapy, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan

    Susuma Tazuma Md, Phd

Authors
  1. Yoshihiro Numata
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  2. Susuma Tazuma Md, Phd
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  3. Yoshitaka Ueno
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  4. Tomoji Nishioka Md, Phd
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  5. Hideyuki Hyogo Md, Phd
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  6. Kazuaki Chayama Md, Phd
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Correspondence to Susuma Tazuma Md, Phd.

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Numata, Y., Tazuma, S., Ueno, Y. et al. Therapeutic Effect of Repeated Natural Killer T Cell Stimulation in Mouse Cholangitis Complicated by Colitis. Dig Dis Sci 50, 1844–1851 (2005). https://doi.org/10.1007/s10620-005-2949-2

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  • DOI: https://doi.org/10.1007/s10620-005-2949-2

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