Abstract
Automation of cell culture processes is necessary to achieve scalable and reproducible manufacture of cell-based therapies. CTX0E03 is a near-clinic neural stem cell line for stroke therapy. The transfer of the CTX0E03 manufacture process from a manual to a completely automated process is demonstrated by (a) adapting the manual process to conform to the automated platform and (b) conducted a large scale (20 × T175 flasks) automated CTX0E03 expansion in accordance with the working bank to drug substance lot manufacture schedule. The automated lot was within the GMP release specification for viability, growth rate, nestin immunoreactivity and transcriptome equivalence.
Similar content being viewed by others
References
Archer R, Williams DJ (2005) Why tissue engineering needs process engineering. Nat Biotechnol 23:1353–1355
Joannides A, Fiore-Hériché C, Westmore K (2006) Automated mechanical passaging: a novel and efficient method for human embryonic stem cell expansion. Stem Cells 24:230–235
Lysaught MJ, Jaklenec A, Deweerd E (2008) Great expectations: private sector activity in tissue engineering, regenerative medicine, and stem cell therapeutics. Tissue Eng Part A 14:305–315
Mansbridge J (2006) Commercial considerations in tissue engineering. J Anat 209:527–532
Mason C (2007) Regenerative medicine 2.0. Regen Med 2:11–18
Pollock K, Stroemer P, Patel S, Stevanato L, Hope A, Miljan E, Dong Z, Hodges H, Price J, Sinden JD (2006) A conditionally immortal clonal stem cell line from human cortical neuroepithelium for the treatment of ischemic stroke. Exp Neurol 199:143–155
Terstegge S, Laufenberg I, Pochert J (2007) Automated maintenance of embryonic stem cell cultures. Biotechnol Bioeng 96:195–201
Thomas RJ, Hourd PC, Williams DJ (2008a) Application of process quality engineering techniques to improve the understanding of the in vitro processing of stem cells for therapeutic use. J Biotechnol 136:148–155
Thomas RJ, Anderson D, Chandra A, Smith NM, Young LE, Williams D, Denning C (2008b) Automated, scalable culture of human embryonic stem cells in feeder-free conditions. Biotech Bioeng (early view online)
Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3(7):research0034.1-0034.11
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Thomas, R.J., Hope, A.D., Hourd, P. et al. Automated, serum-free production of CTX0E03: a therapeutic clinical grade human neural stem cell line. Biotechnol Lett 31, 1167–1172 (2009). https://doi.org/10.1007/s10529-009-9989-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-009-9989-1