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Antimicrobial effect of photodynamic therapy using a highly pure chlorin e6

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Abstract

The aim of the present study is to evaluate the antimicrobial effect of photodynamic therapy (PDT) using a highly pure chlorin e6 (Ce6), against various pathogenic bacteria. To examine the antimicrobial effect of Ce6-mediated PDT against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Salmonella enterica serovar Typhimurium, inhibition zone formation, CFU quantification, and bacterial viability were evaluated. Inhibition zone analysis showed that Ce6-mediated PDT is very effective to inhibit the growth of S. aureus and P. aeruginosa, but has only minor effect to E. coli and S. Typhimurium, which was dependent on the energy density of laser and dose of Ce6. Ce6-mediated PDT also nearly inhibited the colony formation of S. aureus and P. aeruginosa, and partially inhibited that of E. coli and S. Typhimurium. In addition, the number of viable bacteria decreased greatly after PDT application with LS-chlorin e6 of 10 μM and laser and energy density of 20 J/cm2. These results show that Ce6-mediated PDT can be an effective alternative for antimicrobial treatment.

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Acknowledgements

This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A080160).

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Authors and Affiliations

  1. Department of Biochemistry, College of Medicine, Konyang University, Daejeon, 302-711, Republic of Korea

    Jong-Hwan Park

  2. Department of Pathology, School of Dentistry, Chosun University, 375 Seosuk-dong Dong-gu, Gwangju, 501-759, Republic of Korea

    Yeon-Hee Moon, Sang-Gun Ahn & Jung-Hoon Yoon

  3. Microbiology & Immunology, School of Dentistry, Chosun University, Gwangju, 501-759, Republic of Korea

    Iel-Soo Bang

  4. Department of Life Science, GIST, Gwangju, 500-715, Republic of Korea

    Yong-Chul Kim

  5. Department of Biochemistry, College of Oriental Medicine, Dongguk University, Gyungju, 780-714, Republic of Korea

    Soo-A Kim

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  1. Jong-Hwan Park
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Correspondence to Sang-Gun Ahn or Jung-Hoon Yoon.

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Park, JH., Moon, YH., Bang, IS. et al. Antimicrobial effect of photodynamic therapy using a highly pure chlorin e6. Lasers Med Sci 25, 705–710 (2010). https://doi.org/10.1007/s10103-010-0781-1

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  • DOI: https://doi.org/10.1007/s10103-010-0781-1

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