Abstract
A biopsy is a surgical procedure performed to establish a clear diagnosis of a lesion in order to clarify a clinical diagnostic suspicion. During a biopsy procedure it is fundamental to maintain safe and readable cut margins in order to permit histological visualization of possible marginal infiltrations or malignant transformation of a lesion. The aim of this study was to evaluate the histological peripheral damage caused by application of a KTP (potassium titanium phosphate) laser during oral soft tissue biopsy procedures. A KTP laser (λ 532 nm) at different power settings and fluences was used to obtain 45 samples from pig cadaver tongues. The samples were then subdivided into five groups of nine samples each. A final specimen was taken by scalpel as a control. All samples were put into test tubes containing 10% buffered formalin solution, and were examined separately under an optical microscope by two pathologists to evaluate the peripheral thermal damage induced by the laser. In all specimens the cut edges of the incision were free from histological artefacts, especially when lower settings were applied. Statistical analysis showed no differences among the groups. The KTP laser demonstrated surgical effectiveness and caused little peripheral damage to the cut edges, and therefore would always allow a safe histological diagnosis to be obtained.
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Rizoiu IM, Eversole LR, Kimmel AI (1996) Effects of an erbium, chromium: yttrium, scandium, gallium, garnet laser on mucocutaneous soft tissues. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 82:386–395
Romeo U, Del Vecchio A, Ripari F, Palaia G, Chiappafreddo C, Tenore G, Visca P (2007) Effects of different laser devices on oral soft tissues: in vitro experience. J Oral Laser Appl 7(3):155–159
Hernández Fernández C, Subirá Rios D, Bueno Chomón G, Tabares Jiménez J (2008) Prostate and KTP laser. Arch Esp Urol 61(9):1023–1027
Kishimoto Y, Hirano S, Kato N, Suehiro A, Kanemaru S, Ito J (2008) Endoscopic KTP laser photocoagulation therapy for pharyngolaryngeal venous malformations in adults. Ann Otol Rhinol Laryngol 117(12):881–885
Dabis R, Rosbotham J, Jones L, Knowles S, Harland CC (2006) Potassium titanyl phosphate (KTP) laser treatment for molluscum contagiosum. J Dermatol Treat 17(1):45–47
Miyazaki H, Kato J, Watanabe H, Harada H, Kakizaki H, Tetsumura A, Sato A, Omura K (2009) Intralesional laser treatment of voluminous vascular lesions in the oral cavity. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 107(2):164–172
Schoop U, Kluger W, Dervisbegovic S, Goharkhay K, Wernisch J, Georgopoulos A, Sperr W, Moritz A (2006) Innovative wavelengths in endodontic treatment. Lasers Surg Med 38(6):624–630
Zhang C, Wang X, Kinoshita J, Zhao B, Toko T, Kimura Y, Matsumoto K (2007) Effects of KTP laser irradiation, diode laser, and LED on tooth bleaching: a comparative study. Photomed Laser Surg 25(2):91–95
Romeo U, Palaia G, Botti R, Leone V, Rocca JP, Polimeni A (2009) Non-surgical periodontal therapy assisted by potassium-titanyl-phosphate laser: a pilot study. Laser Med Sci. doi:10.1007/s10103-009-0738-4
Zhang L, Poh CF, Lam WL, Epstein JB, Cheng X, Zhang X, Priddy R, Lovas J, Le ND, Rosin MP (2001) Impact of localized treatment in reducing risk of progression of low-grade oral dysplasia: molecular evidence of incomplete resection. Oral Oncol 37(6):505–512
Ma G, Sano K, Ikeda H, Inokuchi T (1999) Promotional effect of CO2 laser and scalpel incision on 4-NQO-induced premalignant lesions of mouse tongue. Laser Surg Med 25(3):207–212
Kimura Y, Wilder-Smith P, Matsumoto K (2000) Lasers in endodontics: a review. Int Endod J 33:173–185
Stern RH, Sognnaes RF (1964) Laser beam effect on dental hard tissues. J Dent Res 43:873
Conrado LA, Munin E, Frois IM, Zingaro RA (2004) Root apex sealing with different filling materials photopolymerized with fiber optic-delivered argon laser light. Lasers Med Sci 19:95–99
Matsumoto K, Suzuki H, Usami Y, Hattori M, Komoro T (2008) Histological evaluation of artifacts in tongue tissue produced by the CO2 laser and the electrotome. Photomed Laser Surg 26(6):573–577
Vivek V, Jayasree RS, Balan A, Sreelatha KT, Gupta AK (2008) Three-year follow-up of oral leukoplakia after neodymium:yttrium aluminum garnet (Nd:YAG) laser surgery. Lasers Med Sci 23(4):375–379
Ergun S, Mete O, Yeşil S, Tanyeri H (2009) Solitary angiokeratoma of the tongue treated with diode laser. Lasers Med Sci 24(1):123–125
Rosa DS, Aranha AC, Eduardo Cde P, Aoki A (2007) Esthetic treatment of gingival melanin hyperpigmentation with Er:YAG laser: short-term clinical observations and patient follow-up. J Periodontol 78(10):2018–2025
Zola M, Rosenberg D, Anakwa K (2006) Treatment of a ranula using an Er,Cr:YSGG laser. J Oral Maxillofac Surg 64(5):823–827
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Romeo, U., Palaia, G., Del Vecchio, A. et al. Effects of KTP laser on oral soft tissues. An in vitro study. Lasers Med Sci 25, 539–543 (2010). https://doi.org/10.1007/s10103-010-0756-2
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DOI: https://doi.org/10.1007/s10103-010-0756-2