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Preparation of poly(vinyl alcohol)/DNA hydrogels via hydrogen bonds formed on ultra-high pressurization and controlled release of DNA from the hydrogels for gene delivery

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Abstract

Poly(vinyl alcohol) (PVA) hydrogels interacting with DNA mediated by hydrogen bonds (PVA/DNA hydrogel) were developed using ultra-high pressure (UHP) technology. The goal was to create a new method of gene delivery by controlled release of DNA. Mixed solutions of DNA and PVA at various concentrations were pressurized at 10 000 atmospheres at 37°C for 10 min. PVA/DNA hydrogels with good formability were produced at PVA concentrations of more than 5% w/v. The presence of DNA in the obtained hydrogels was confirmed by spectroscopic analysis and nucleic acid dye staining. DNA release from the hydrogels was investigated using PVA/DNA hydrogel samples of 5% and 10% w/v formed by UHP treatment or by conventional freeze–thaw methods. The DNA release curves from both types of samples showed a rapid phase in the initial 15 h followed by a sustained release phase. However, there was a difference in the amount of DNA released. Less DNA was released by the pressurized hydrogels than by the freeze–thaw hydrogels. Also, the cumulative amount of DNA released decreased as the PVA content in the hydrogels increased. These results indicate that DNA release from the hydrogels can be modulated by changing the preparation method and the PVA content. Furthermore, it was demonstrated that DNA release could be controlled by varying the amount and duration of pressurizing used to form the hydrogels. Intact fractions of plasmid DNA released from the hydrogels were separated by agarose gel electrophoretic analysis. These results suggest that, using controlled release, DNA from PVA/DNA hydrogels formed by UHP treatment can be transfected into cells.

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References

  1. T Nowak K Nishida S Shimoda Y Konno K Ichinose M Sakakibara M Shichiri N Nakabayashi K Ishihara (2000) ArticleTitleBiocompatiblility of MPC: in vivo evaluation for clinical application J Artif Organs 1 39–46 Occurrence Handle10.1007/BF02479925

    Article  Google Scholar 

  2. DJ Glover HJ Lipps DA Jans (2005) ArticleTitleTowards safe, non-viral therapeutic gene expression in humans Nat Rev Genet 6 299–310 Occurrence Handle15761468 Occurrence Handle10.1038/nrg1577 Occurrence Handle1:CAS:528:DC%2BD2MXivFGisrY%3D

    Article  PubMed  CAS  Google Scholar 

  3. S Zhang Y Xu B Wan W Qiao D Liu Z Li (2004) ArticleTitleCationic compounds used in lipoplexes and polyplexes for gene delivery J Control Release 100 165–180 Occurrence Handle15544865 Occurrence Handle10.1016/j.jconrel.2004.08.019 Occurrence Handle1:CAS:528:DC%2BD2cXps1Oru7w%3D

    Article  PubMed  CAS  Google Scholar 

  4. U Lungwitz M Breunig T Blunk A Göpferich (2005) ArticleTitlePolyethylenimine-based non-viral gene delivery systems Eur J Pharm Biopharm 60 247–266 Occurrence Handle15939236 Occurrence Handle10.1016/j.ejpb.2004.11.011 Occurrence Handle1:CAS:528:DC%2BD2MXltFCrsrs%3D

    Article  PubMed  CAS  Google Scholar 

  5. C Dufes IF Uchegbu AG Scatzlein (2005) ArticleTitleDendrimers in gene delivery Adv Drug Deliv Rev 57 2117–2202 Occurrence Handle10.1016/j.addr.2005.09.017

    Article  Google Scholar 

  6. T Kimura T Yamaoka R Iwase A Murakami (2002) ArticleTitleEffect of physicochemical properties of polyplexes composed of chemically modified PL derivatives on transfection efficiency in vitro Macromol Biosci 2 437–446 Occurrence Handle10.1002/mabi.200290004 Occurrence Handle1:CAS:528:DC%2BD3sXitFWqsrk%3D

    Article  CAS  Google Scholar 

  7. S Futaki (2005) ArticleTitleMembrane-permeable arginine-rich peptides and the translocation mechanisms Adv Drug Deliv Rev 57 547–558 Occurrence Handle15722163 Occurrence Handle10.1016/j.addr.2004.10.009 Occurrence Handle1:CAS:528:DC%2BD2MXhslWgu7c%3D

    Article  PubMed  CAS  Google Scholar 

  8. T Reschel Č Koňák D Oupickỳ LW Seymour K Ulbrich (2002) ArticleTitlePhysical properties and in vitro transfection efficiency of gene delivery vectors based on complexes of DNA with synthetic polycations J Control Release 81 201–217 Occurrence Handle11992692 Occurrence Handle10.1016/S0168-3659(02)00045-7 Occurrence Handle1:CAS:528:DC%2BD38XjtFyht78%3D

    Article  PubMed  CAS  Google Scholar 

  9. A Elouahabi JM Ruysschaert (2005) ArticleTitleFormation and intracellular trafficking of lipoplexes and polyplexes Mol Ther 11 336–347 Occurrence Handle15727930 Occurrence Handle10.1016/j.ymthe.2004.12.006 Occurrence Handle1:CAS:528:DC%2BD2MXhsVOjurw%3D

    Article  PubMed  CAS  Google Scholar 

  10. D Fischer Y Li B Ahlemeyer J Krieglstein T Kissel (2006) ArticleTitleIn vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis Biomaterals 24 1121–1131 Occurrence Handle10.1016/S0142-9612(02)00445-3

    Article  Google Scholar 

  11. S Choksakulnimitr S Matsuda H Tokuda Y Takakura M Hashida (1995) ArticleTitleIn vitro cytotoxicity of macromolecules in different cell culture systems J Control Release 34 233–241 Occurrence Handle10.1016/0168-3659(95)00007-U Occurrence Handle1:CAS:528:DyaK2MXlvVWjtbw%3D

    Article  CAS  Google Scholar 

  12. K Sakurai M Mizu S Shinkai (2001) ArticleTitlePolysaccharide–polynucleotide complexes. 2. Complementary polynucleotide mimic behavior of the natural polysaccharide schizophyllan in the macromolecular complex with single-stranded RNA and DNA Biomacromolecules 2 641–650 Occurrence Handle11710016 Occurrence Handle10.1021/bm000121r Occurrence Handle1:CAS:528:DC%2BD3MXkt1Wktrk%3D

    Article  PubMed  CAS  Google Scholar 

  13. T Kimura A Okuno K Miyazaki T Furuzono Y Ohya T Ouchi S Mutsuo H Yoshizawa Y Kitamura T Fujisato A Kishida (2004) ArticleTitleNovel PVA-DNA nonoparticles prepared by ultra high pressure technology for gene delivery Mater Sci Eng C 24 797–801 Occurrence Handle10.1016/j.msec.2004.08.046

    Article  Google Scholar 

  14. E Doi A Shimizu N Kitabatake (1993) Gel-sol transition of ovalbumin by high pressure R Hayashi (Eds) High pressure bioscience and food science Sanei Press Kyoto 171–177

    Google Scholar 

  15. LD Shea E Smiley J Bonadio DJ Mooney (1999) ArticleTitleDNA delivery from polymer matrices for tissue engineering Nat Biotech 17 551–554 Occurrence Handle10.1038/9853 Occurrence Handle1:CAS:528:DyaK1MXjs1Kgurk%3D

    Article  CAS  Google Scholar 

  16. KW Chun JB Lee SH Kim TG Rark (2005) ArticleTitleControlled release of plasmid DNA from photo-cross-linked pluronic hydrogels Biomaterials 26 3319–3326 Occurrence Handle15603827 Occurrence Handle10.1016/j.biomaterials.2004.07.055 Occurrence Handle1:CAS:528:DC%2BD2cXhtVyrsLrF

    Article  PubMed  CAS  Google Scholar 

  17. T Ochiya Y Takahama S Nagahara Y Sumita A Hisada H Itoh Y Nagai M Terada (1999) ArticleTitleNew delivery system for plasmid DNA in vivo using atelocollagen as a carrier material the Minipellet Nat Med 5 707–710 Occurrence Handle10371512 Occurrence Handle10.1038/9560 Occurrence Handle1:CAS:528:DyaK1MXjs1Kltb0%3D

    Article  PubMed  CAS  Google Scholar 

  18. Y Fukunaka K Iwanaga K Morimoto M Kakemi Y Tabata (2005) ArticleTitleControlled release of plasmid DNA from cationized gelatin hydrogels based on hydrogel degradation Biomaterials 26 3319–3326 Occurrence Handle10.1016/j.biomaterials.2004.07.055

    Article  Google Scholar 

  19. T Kushibiki R Tomoshige Y Fukunaka M Kakemi Y Tabata (2003) ArticleTitleIn vivo release and gene expression of plasmid DNA by hydrogels of gelatin with different cationization extents J Control Release 90 207–216 Occurrence Handle12810303 Occurrence Handle10.1016/S0168-3659(03)00197-4 Occurrence Handle1:CAS:528:DC%2BD3sXksFKkurw%3D

    Article  PubMed  CAS  Google Scholar 

  20. H Miyashita S Shimmura H Kobayashi T Taguchi K Asano-Kato Y Uchino M Kato J Shimazaki J Tanaka K Tsubota (2006) ArticleTitleCollagen-immobilized poly (vinyl alcohol) as an artificial cornea scaffold that supports a stratified corneal epithelium J Biomed Mater Res Part B: Appl Biomater 76B 56–63 Occurrence Handle10.1002/jbm.b.30332 Occurrence Handle1:CAS:528:DC%2BD28Xptl2guw%3D%3D

    Article  CAS  Google Scholar 

  21. SH Hyon WI Cha Y Ikada (1989) ArticleTitlePreparation of transparent poly (vinyl alcohol) hydrogel Polymer Bull 22 119–122 Occurrence Handle10.1007/BF00255200 Occurrence Handle1:CAS:528:DyaK3cXotFKq

    Article  CAS  Google Scholar 

  22. K Yamamoto T Furuzono A Kishida S Mustuo H Yoshizawa Y Kitamura (2002) ArticleTitleFormation of a supramolecular assembly of poly (vinyl alcohol) by ultrahigh pressure Meeting Report of the Poval Committee 121 25–26

    Google Scholar 

  23. E Walter K Moelling HP Pavolvich (1999) ArticleTitleMicroencapsulation of DNA using poly (d,l-lactide-co-glycolide): stability issues and release characteristics J Control Release 61 361–374 Occurrence Handle10477808 Occurrence Handle10.1016/S0168-3659(99)00151-0 Occurrence Handle1:CAS:528:DyaK1MXls1ylsrc%3D

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-0062, Japan

    Tsuyoshi Kimura PhD, Kwangwoo Nam PhD & Akio Kishida PhD

  2. Department of Medical Engineering, Suzuka University of Medical Science, Suzuka, Japan

    Sayaka Iwai & Toshiyuki Moritan PhD

  3. Department of Environmental Chemistry and Materials, Okayama University, Okayama, Japan

    Shingo Mutsuo & Hidekazu Yoshizawa PhD

  4. Department of Biomedical Engineering, National Cardiovascular Center Research Institute, Osaka, Japan

    Masahiro Okada PhD & Tsutomu Furuzono PhD

  5. Department of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center Research Institute, Osaka, Japan

    Tosihya Fujisato PhD

Authors
  1. Tsuyoshi Kimura PhD
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  2. Sayaka Iwai
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  3. Toshiyuki Moritan PhD
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  4. Kwangwoo Nam PhD
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  5. Shingo Mutsuo
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  6. Hidekazu Yoshizawa PhD
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  7. Masahiro Okada PhD
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  8. Tsutomu Furuzono PhD
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  9. Tosihya Fujisato PhD
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  10. Akio Kishida PhD
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Corresponding author

Correspondence to Akio Kishida PhD.

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Kimura, T., Iwai, S., Moritan, T. et al. Preparation of poly(vinyl alcohol)/DNA hydrogels via hydrogen bonds formed on ultra-high pressurization and controlled release of DNA from the hydrogels for gene delivery. J Artif Organs 10, 104–108 (2007). https://doi.org/10.1007/s10047-006-0367-7

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  • DOI: https://doi.org/10.1007/s10047-006-0367-7

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