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Quantitative elemental analysis on aluminum accumulation by HVTEM-EDX in liver tissues of mice orally administered with aluminum chloride

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Abstract

Quantitative elemental analysis on Al was carried out by high-accelerating voltage transmission electron microscopy (HVTEM) equipped with energy-dispersive X-ray microanalysis (EDX) using an accelerating voltage at 300 kV with high permeability in 1-μm-thick samples obtained from mice administered with aluminum chloride solution for 3, 9, and 17 weeks. By light microscopic observation, no morphological changes were observed in the hepatocytes and macrophages in the liver tissues of mice that were administered with excess Al as compared with the normal control mice. In contrast, by electron microscopic observation, ultrastructural changes were observed in the lysosomes in the hepatocytes as well as the pinocytotic vesicles in the macrophages in the experimental animals. Therefore, the concentrations of Al detected in lysosomes in hepatocytes and pinocytotic vesicles in macrophages of livers of mice administered with Al were measured in relationship to those administration periods. Moreover, transitional changes of hepatocyte lysosome ratios by image analysis and the macrophage counts in the unit area increased in liver tissues of mice administered with Al as compared with normal control mice. From the results, it was demonstrated that hepatocyte lysosome ratio and macrophage count increased in liver tissues of treated mice during those short-term excessive Al administration periods. It was also clarified that the concentrations of Al in both hepatocytes and macrophages increased as observed by HVTEM-EDX. In conclusion, Al accumulated in hepatocytes and macrophages at 3 and 9 weeks administration, while the ultrastructural changes remained in the hepatocytes and macrophages. In contrast, Al concentration did not increase in the liver at 17 weeks administration.

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References

  1. RA Yokel PJ McNamara (2001) ArticleTitleAluminum toxicokinetics: an update minireview Pharmacol Toxicol 88 159–167 Occurrence Handle11322172 Occurrence Handle1:CAS:528:DC%2BD3MXjtVahtbc%3D Occurrence Handle10.1034/j.1600-0773.2001.d01-98.x

    Article  PubMed  CAS  Google Scholar 

  2. TP Flaten (2001) ArticleTitleAluminum as a risk factor in Alzheimer's disease, with emphasis on drinking water Brain Res Bull 55 187–196 Occurrence Handle11470314 Occurrence Handle1:CAS:528:DC%2BD3MXltlOitLk%3D Occurrence Handle10.1016/S0361-9230(01)00459-2

    Article  PubMed  CAS  Google Scholar 

  3. AC Alfrey GR LeGendre WD Kaehny (1976) ArticleTitleThe dialysis encephalopathy syndrome. Possible aluminum intoxication N Engl J Med 294 184–188 Occurrence Handle1244532 Occurrence Handle1:STN:280:DyaE28%2FnsFGqsg%3D%3D Occurrence Handle10.1056/NEJM197601222940402

    Article  PubMed  CAS  Google Scholar 

  4. M Wilhelm D Jäger FK Ohnesorge (1990) ArticleTitleAluminum toxicokinetics Pharmacol Toxicol 66 4–9 Occurrence Handle2408032 Occurrence Handle1:CAS:528:DyaK3cXhtFeltLg%3D Occurrence Handle10.1111/j.1600-0773.1990.tb00692.x

    Article  PubMed  CAS  Google Scholar 

  5. E Lydersen S Lofgren (2002) ArticleTitlePotential effects of metals in reacidified limed water bodies in Norway and Sweden Environ Monit Assess 73 IssueID2 155–178 Occurrence Handle11878646 Occurrence Handle1:CAS:528:DC%2BD38XhvFKltr8%3D Occurrence Handle10.1023/A:1013002904977

    Article  PubMed  CAS  Google Scholar 

  6. J Savory C Exley WF Forbes Y Huang JG Joshi T Kruck DR McLachlan I Wakayama (1996) ArticleTitleCan the controversy of the role of aluminum in Alzheimer's disease be resolved? What are the suggested approaches to this controversy and methodological issues to be considered? J Toxicol Environ Health 48 615–636 Occurrence Handle8772802 Occurrence Handle1:CAS:528:DyaK28XltlKitro%3D Occurrence Handle10.1080/009841096161104

    Article  PubMed  CAS  Google Scholar 

  7. CN Martyn DJ Barker C Osmond EC Harris JA Edwardson RF Lacey (1989) ArticleTitleGeographical relation between Alzheimer's disease and aluminum in drinking water Lancet 14 59–62

    Google Scholar 

  8. DR McLachlan C Bergeron JE Smith D Boomer SL Rifat (1996) ArticleTitleRisk for neuropathologically confirmed Alzheimer's disease and residual aluminum in municipal drinking water employing weighted residential histories Neurology 46 401–405 Occurrence Handle8614502 Occurrence Handle1:STN:280:DyaK287otF2ltg%3D%3D

    PubMed  CAS  Google Scholar 

  9. A Warley (1990) ArticleTitleStandards for the application of X-ray microanalysis to biological specimens J Microsc 157 135–147 Occurrence Handle2179559 Occurrence Handle1:CAS:528:DyaK3cXitFeiu7Y%3D

    PubMed  CAS  Google Scholar 

  10. K Kametani R Ichikawa T Nagata (1998) ArticleTitleX-ray microanalysis of the secretory granules in goblet cells of mouse intestinal tracts: change with age Med Electron Microsc 31 107–114 Occurrence Handle1:CAS:528:DyaK1cXltFGis7o%3D Occurrence Handle10.1007/BF01557788

    Article  CAS  Google Scholar 

  11. J Jingtao S Sato (1999) ArticleTitleDetection of calcium and aluminum in pyramidal neuron in the gerbil hippocampal CA1 region following reported brief cerebral ischemia: X-ray microanalysis Med Electron Microsc 32 161–166 Occurrence Handle11810440 Occurrence Handle1:CAS:528:DC%2BD3cXisFWntr4%3D Occurrence Handle10.1007/s007950050023

    Article  PubMed  CAS  Google Scholar 

  12. DP Perl AR Brody (1980) ArticleTitleAlzheimer's disease: X-ray spectrometric evidence of aluminum accumulation in neurofibrillary tangle-bearing neurons Science 208 297–299 Occurrence Handle7367858 Occurrence Handle1:CAS:528:DyaL3cXhvFGqu7c%3D

    PubMed  CAS  Google Scholar 

  13. R Jacobs T Duong RE Jones GA Trapp AB Scheibel (1989) ArticleTitleA reexamination of aluminum in Alzheimer's disease: Analysis by energy dispersive x-ray microprobe and flameless atomic absorption spectrophotometry Can J Neurol Sci 16 498–503 Occurrence Handle2804812 Occurrence Handle1:STN:280:DyaK3c%2FivF2ntA%3D%3D

    PubMed  CAS  Google Scholar 

  14. K Kametani (2002) ArticleTitleDetection of aluminum by energy dispersive X-ray microanalysis at high accelerating voltages with semi-thin sections of biological sample J Electron Microsc 51 265–274 Occurrence Handle1:CAS:528:DC%2BD38Xms1ykur4%3D Occurrence Handle10.1093/jmicro/51.4.265

    Article  CAS  Google Scholar 

  15. K Kametani K Suzuki T Nagata (2003) ArticleTitleX-ray microanalysis at high accelerating voltage detecting aluminum accumulation in mouse kidney after short-term aluminum administration Acta Histochem Cytochem 36 221–230 Occurrence Handle1:CAS:528:DC%2BD3sXmvFCqtbs%3D Occurrence Handle10.1267/ahc.36.221

    Article  CAS  Google Scholar 

  16. K Kametani T Nagata (2005) ArticleTitleDemonstration of aluminum in mouse tissues by X-ray microanalysis and energy spectroscopic imaging with electron energy loss spectroscopy Ann Microsc 5 19–27

    Google Scholar 

  17. ES Reynolds (1963) ArticleTitleThe use of lead citrate at high pH as an electron opaque stain in electron microscopy J Cell Biol 17 208–212 Occurrence Handle13986422 Occurrence Handle1:CAS:528:DyaF3sXktVClu70%3D Occurrence Handle10.1083/jcb.17.1.208

    Article  PubMed  CAS  Google Scholar 

  18. AJ Spencer JA Wood HC Saunders MS Freeman CJ Lote (1995) ArticleTitleAluminum deposition in liver and kidney following acute intravenous administration of aluminum chloride or citrate in conscious rats Hum Exp Toxicol 14 787–794 Occurrence Handle8562118 Occurrence Handle1:CAS:528:DyaK2MXpslSltLk%3D Occurrence Handle10.1177/096032719501401002

    Article  PubMed  CAS  Google Scholar 

  19. T Maitani H Kubota N Hori K Yoshihira M Takeda (1994) ArticleTitleDistribution and urinary excretion of aluminum injected with several organic acids into mice: relationship with chemical state in serum studied by the HPLC-ICP method J Appl Toxicol 14 257–261 Occurrence Handle7963237 Occurrence Handle1:CAS:528:DyaK2cXlt1Ohsrs%3D

    PubMed  CAS  Google Scholar 

  20. CJ Lote H Saunders (1991) ArticleTitleAluminum: gastrointestinal absorption and renal excretion Clin Sci 81 289–295 Occurrence Handle1655328 Occurrence Handle1:STN:280:DyaK38%2Fgs1Cktg%3D%3D

    PubMed  CAS  Google Scholar 

  21. C De Duve T De Barsy B Poole A Trouet P Tulkens F Van Hoof (1974) ArticleTitleCommentary. Lysosomotropic agents Biochem Pharmacol 15 2495–2531 Occurrence Handle10.1016/0006-2952(74)90174-9

    Article  Google Scholar 

  22. P Schneider TA Korolenko U Busch (1997) ArticleTitleA review of drug-induced lysosomal disorders of the liver in man and laboratory animals Microsc Res Tech 36 253–275 Occurrence Handle9140926 Occurrence Handle1:CAS:528:DyaK2sXjtFOis7o%3D Occurrence Handle10.1002/(SICI)1097-0029(19970215)36:4<253::AID-JEMT4>3.0.CO;2-N

    Article  PubMed  CAS  Google Scholar 

  23. JB Cannata I Fremández-Soto MJ Fremández-Menendez JL Fremández-Martin SJ McGregor JH Borck D Halls (1991) ArticleTitleRole of iron metabolism in absorption and cellular uptake of aluminum Kidney Int 39 799–803 Occurrence Handle2051739 Occurrence Handle1:CAS:528:DyaK3MXlsFOltrc%3D

    PubMed  CAS  Google Scholar 

  24. JP Berry (1996) ArticleTitleThe role of lysosomes in the selective concentration of mineral elements. A microanalytical study Cell Mol Biol 42 395–411 Occurrence Handle8793193 Occurrence Handle1:CAS:528:DyaK28Xjs1Ohur4%3D

    PubMed  CAS  Google Scholar 

  25. MR Wills CD Hewitt BC Sturgill J Savory MM Herman (1993) ArticleTitleLong-term oral or intravenous aluminum administration in rabbits. I. Renal and hepatic changes Ann Clin Lab Sci 23 1–16 Occurrence Handle7679266 Occurrence Handle1:CAS:528:DyaK3sXhs1alt7w%3D

    PubMed  CAS  Google Scholar 

  26. MR Wills CD Hewitt J Savory MM Herman (1993) ArticleTitleLong-term oral aluminum administration in rabbits. II. Brain and other organs Ann Clin Lab Sci 23 17–23 Occurrence Handle8430996 Occurrence Handle1:CAS:528:DyaK3sXhs1alt70%3D

    PubMed  CAS  Google Scholar 

  27. T Nagata (2003) ArticleTitleThe utility value of high voltage electron microscopy for X-ray microanalysis Acta Histochem Cytochem 36 299–315 Occurrence Handle1:CAS:528:DC%2BD3sXpt1ejsbY%3D Occurrence Handle10.1267/ahc.36.299

    Article  CAS  Google Scholar 

  28. T Nagata (2004) ArticleTitleX-ray microanalysis of biological specimens by high voltage electron microscopy Prog Histochem Cytochem 39 185–320 Occurrence Handle15622702 Occurrence Handle1:CAS:528:DC%2BD2MXhtlyju7o%3D Occurrence Handle10.1016/j.proghi.2004.07.001

    Article  PubMed  CAS  Google Scholar 

  29. T Nagata (2004) ArticleTitlePrinciples and applications of X-ray microanalysis to biological specimens by high voltage electron microscopy ARBS (Ann Rev Biomed Sci) 6 13–78 Occurrence Handle1:CAS:528:DC%2BD28XksVWhtLY%3D

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Instrumental Analysis, Research Center for Human Environmental Sciences, Shinshu University, and General Research Laboratory, Shinshu University School of Medicine, Matsumoto, 390-8621, Japan

    Kiyokazu Kametani

  2. Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, and Department of Anatomy, Shinshu Institute of Alternative Medicine, Nagano, Japan

    Tetsuji Nagata

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  1. Kiyokazu Kametani
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  2. Tetsuji Nagata
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Correspondence to Kiyokazu Kametani.

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Kametani, K., Nagata, T. Quantitative elemental analysis on aluminum accumulation by HVTEM-EDX in liver tissues of mice orally administered with aluminum chloride. Med Mol Morphol 39, 97–105 (2006). https://doi.org/10.1007/s00795-006-0316-9

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  • DOI: https://doi.org/10.1007/s00795-006-0316-9

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