New Spectrophotometric Methods for Microdetermination of Fluoxacillin and Cloxacillin in Pure and Dosage Forms

Abstract.

 Two simple, rapid and sensitive methods for the microdetermination of penicillin derivatives are described. The studied compounds are fluoxacillin and cloxacillin. The methods are based on the reaction of these drugs as n-electron donors with either 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) or 7,7, 8,8-tetracyanoquinodimethane (TCNQ) as π-acceptors, to give a highly coloured radical anion. The coloured products were quantified spectrophotometrically at 460 and 840 nm for DDQ and TCNQ, respectively. The proposed methods were applied successfully to the determination of the two penicillins investigated, either in pure or dosage forms, with good accuracy and precision. The results were compared with those given by the official method.