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Biosensor based on horseradish peroxidase modified carbon nanotubes for determination of 2,4-dichlorophenol

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Abstract.

A novel and convenient strategy is presented for determination of 2,4-dichlorophenol (2,4-DCP). Horseradish peroxidase (HRP) was self-assembled on a multiwalled carbon nanotubues (MWNTs) modified glassy carbon (GC) electrode. In the presence of hydrogen peroxide (H2O2), 2,4-DCP can be oxidized at this enzyme electrode and the reduction current is proportional to the concentration of the 2,4-DCP. The method showed good linearly for 1.0 × 10−6–1.0 × 10−4 M 2,4-DCP with a detection limit of 3.8 × 10−7 M under the optimal conditions. The peak current of the HRP-MWNTs-GC electrode decreased by about 15% over two weeks. The mechanism of the enzyme biosensor was also studied, and a kinetic equation was derived. The performance of the electrode was verified by determination of 2,4-DCP in environmental water.

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Authors and Affiliations

  1. Life and Environmental Science College, Shanghai Normal University, Shanghai, P.R. China

    Shasheng Huang, Yongxia Qu, Ruina Li, Jian Shen & Liwei Zhu

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  1. Shasheng Huang
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  2. Yongxia Qu
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  3. Ruina Li
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  4. Jian Shen
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  5. Liwei Zhu
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Correspondence to Shasheng Huang.

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Correspondence: Shasheng Huang, Life and Environmental Science College, Shanghai Normal University, Shanghai 200234, P.R. China

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Huang, S., Qu, Y., Li, R. et al. Biosensor based on horseradish peroxidase modified carbon nanotubes for determination of 2,4-dichlorophenol. Microchim Acta 162, 261–268 (2008). https://doi.org/10.1007/s00604-007-0872-2

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  • DOI: https://doi.org/10.1007/s00604-007-0872-2

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