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Firefly luciferase as a reporter of regulated gene expression in higher plants

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Abstract

The firefly luciferase, assayedin vivo with a low-light video camera, acts as a non-invasive, real-time reporter of the temporal and spatial regulation of gene expression in single plants. Furthermore, the sensitivity of the luciferase assay in extracts of transformed plant tissue makes it a particularly useful marker in transient or stable transformation experiments.

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Abbreviations

GUS:

β-glucuronidase

ddH2O:

double-distilled water

BSA:

bovine serum albumin

LAR:

luciferase assay reagent

CLR:

cell lysis reagent

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Millar, A.J., Short, S.R., Hiratsuka, K. et al. Firefly luciferase as a reporter of regulated gene expression in higher plants. Plant Mol Biol Rep 10, 324–337 (1992). https://doi.org/10.1007/BF02668909

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