Abstract
The firefly luciferase, assayedin vivo with a low-light video camera, acts as a non-invasive, real-time reporter of the temporal and spatial regulation of gene expression in single plants. Furthermore, the sensitivity of the luciferase assay in extracts of transformed plant tissue makes it a particularly useful marker in transient or stable transformation experiments.
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Abbreviations
- GUS:
-
β-glucuronidase
- ddH2O:
-
double-distilled water
- BSA:
-
bovine serum albumin
- LAR:
-
luciferase assay reagent
- CLR:
-
cell lysis reagent
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Millar, A.J., Short, S.R., Hiratsuka, K. et al. Firefly luciferase as a reporter of regulated gene expression in higher plants. Plant Mol Biol Rep 10, 324–337 (1992). https://doi.org/10.1007/BF02668909
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DOI: https://doi.org/10.1007/BF02668909