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Chitinolytic enzymes: their contribution to basic and applied research

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Abstract

After cellulose, chitin is the second most abundant renewable resource available in nature. Marine invertebrates and fungal biomass are the two main sources of chitinous waste, which is commercially exploited. The enzymes involved in chitin degradation have been particularly well studied. Such enzymes have applications in ultrastructural studies, in the preparation of chitooligosaccharides which show anti-tumour activity, as biocontrol agents and in single-cell protein production. Here, the contribution chitin enzymology can make to basic and applied research is discussed.

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References

  • Alfonso, C., Martinez, M.J. & Reyes, F. 1991 Degradation of chitosan in the autolysis of mucorales. Mycology Research 95, 217–219.

    Google Scholar 

  • Anuratha, C.S., Huang, J.K., Pingali, A. & Muthukrishnan, S. 1992 Isolation and characterization of chitinase and its cDNA clone from rice. Journal of Plant Biochemistry and Biotechnology 1, 5–10.

    Google Scholar 

  • Aribisala, O.A. & Gooday, G.W. 1978 Properties of chitinase from Vibrio alginolyticus, as assayed with the chromogenic substrate 3,4-dinitrophenyl tetra-N-acetylchitotetraoside. Biochemical Society Transactions 6, 568–569.

    Google Scholar 

  • Barrows-Broaddus, J. & Kerr, T.J. 1981 Inhibition of Fusarium moniliforme var. subglutinans, the causal agent of pitch canker, by the soil bacterium Arthrobacter sp. Canadian Journal of Microbiology 27, 20–27.

    Google Scholar 

  • Benhamou, N. 1988 Ultrastructural localization of carbohydrates in the cell walls of two pathogenic fungi: a comparative study. Mycologia 80, 324–337.

    Google Scholar 

  • Benhamou, N. 1989 Cytochemical localization of β-(1 → 4)-d-glucans in plant and fungal cells using an exoglucanase-gold complex. Electron Microscopy Review 2, 123–138.

    Google Scholar 

  • Benhamou, N. & Asselin, A. 1989 Attempted localization of a substrate for chitinase in plant cells reveals abundant N-acetyl-d-glucosamine residues in secondary walls. Biology of the Cell 67, 341–350.

    Google Scholar 

  • Boller, T. & Mauch, F. 1988 Colorimetric assay for chitinase. Methods in Enzymology 161, 430–435.

    Google Scholar 

  • Broglie, K.E., Gaynor, J.J. & Broglie, R.M. 1986 Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris. Proceedings of the National Academy of Science of the United States of America 83, 6820–6824.

    Google Scholar 

  • Cabib, E. 1987 The synthesis and degradation of chitin. Advances in Enzymology 59, 59–101.

    Google Scholar 

  • Cabib, E. 1988 Assay for chitinase using tritiated chitin. Methods in Enzymology 161, 424–426.

    Google Scholar 

  • Davis, J.M., Clarke, H.R.G., BradshawJr., H.D. & Gordon, M.P. 1991 Populus chitinase genes: structure, organization, and similarity of translated sequences to herbaceous plant chitinases. Plant Molecular Biology 17, 631–639.

    Google Scholar 

  • Deshpande, M.V. 1986 Enzymatic degradation of chitin and its biological applications. Journal of Scientific and Industrial Research 45, 273–281.

    Google Scholar 

  • Deshpande, M.V., Eriksson, K.E. & Petterson, L.G. 1984 An assay for selective determination of exo-1,4-β-glucanases in a mixture of cellulolytic enzymes. Analytical Biochemistry 138, 481–487.

    Google Scholar 

  • ElOuakfaoui, S. & Asselin, A. 1992 Multiple forms of chitosanase activities. Phytochemistry 31, 1513–1518.

    Google Scholar 

  • Fink, D., Boucher, I., Denis, F. & Brzezinski, R. 1991 Cloning and expression in Streptomyces lividans of a chitosanase-encoding gene from actinomycetes Kitasatosporia N174 isolated from soil. Biotechnology Letters 13, 845–850.

    Google Scholar 

  • Flach, J., Pilet, P.-E. & Jolles, P. 1992 What's new in chitinase research? Experientia 48, 701–716.

    Google Scholar 

  • Fuchs, R.L., McPherson, S.A. & Drahos, D.J. 1986 Cloning of a Serratia marcescens gene encoding chitinase. Applied and Environmental Microbiology 51, 504–509.

    Google Scholar 

  • Fukuda, Y., Ohme, M. & Shinshi, H. 1991 Gene structure and expression of a tobacco endochitinase gene in suspension-cultured tobacco cells. Plant Molecular Biology 16, 1–10.

    Google Scholar 

  • Gabriel, C.J. & Cook, R.J. 1990 Biological control of plant pathogens. FAO Plant Protection Bulletin 38, 95–99.

    Google Scholar 

  • Gaynor, J.J. 1988 Primary structure of an endochitinase mRNA from Solanum tuberosum. Nucleic Acids Research 16, 521–5210.

    Google Scholar 

  • Gooday, G.W. 1990 Physiology of microbial degradation of chitin and chitosan. Biodegradation 1, 177–190.

    Google Scholar 

  • Gooday, G.W. 1991 Chitinases. In Enzymes in Biomass Conversion, ACS Symposium No. 460, eds Letham, G.F. & Himmel, M.E. pp. 478–485. Washington DC: American Chemical Society.

    Google Scholar 

  • Grenier, J., Benhamou, N. & Asselin, A. 1991 Colloidal goldcomplexed chitosanase: a new probe for ultrastructural localization of chitosan in fungi. Journal of General Microbiology 137, 2007–2015.

    Google Scholar 

  • Hamlyn, P.F., Bradshaw, R.E., Mellon, F.M., Santiago, C.M., Wilson, J.M. & Peberdy, J.F. 1981 Efficient protoplast isolation from fungi using commercial enzymes. Enzyme and Microbial Technology 3, 321–325.

    Google Scholar 

  • Hara, S., Yamamura, Y., Fujii, Y., Mega, T. & Ikenaka, T. 1989 Purification and characterization of chitinase produced by Streptomyces erythraeus. Journal of Biochemistry 105, 484–489.

    Google Scholar 

  • Herwig, R.P., Pellerin, N.B., Irgens, R.L., Maki, J.S. & Staley, J.T. 1988 Chitinolytic bacteria and chitin mineralization in the marine waters and sediments along the antarctic peninsula. FEMS Microbiology Ecology 53, 101–112.

    Google Scholar 

  • Hood, M.A. 1991 Comparison of four methods for measuring chitinase activity and the application of the 4-MUF assay in aquatic environments. Journal of Microbiological Methods 13, 151–160.

    Google Scholar 

  • Horwitz, M., Reid, J. & Ogrydziak, D. 1984 Genetic improvement of chitinase production by Serratia marcescens. In Chitin, Chitosan and Related Enzymes, ed Zikakis, J.P. pp. 191–208. Orlando: Academic Press.

    Google Scholar 

  • Huynh, Q.K., Hironaka, C.M., Levine, E.B., Smith, C.E., Borgmeyer, J.R. & Shah, D.M. 1992 Antifungal proteins from plants. Purification, molecular cloning, and antifungal properties of chitinases from maize seed. Journal of Biological Chemistry 267, 6635–6640.

    Google Scholar 

  • Izume, M. & Ohtakara, A. 1987 Preparation of d-glucosamine oligosaccharides by the enzymatic hydrolysis of chitosan. Agricultural and Biological Chemistry 51, 1189–1191.

    Google Scholar 

  • Jannatipour, M., Soto-Gil, R.W., Childers, L.C. & Zyskind, J.W. 1987 Translocation of Vibrio harveyi N, N′-diacetylchitobiase to the outer membrane of Escherichia coli. Journal of Bacteriology 169, 3785–3791.

    Google Scholar 

  • Jones, J.D.G., Grady, K.L., Suslow, T.V. & Bedbrook, J.R. 1986 Isolation and characterization of genes encoding two chitinase enzymes from Serratia marcescens. EMBO Journal 5, 467–473.

    Google Scholar 

  • Joshi, S., Kozlowski, M., Selvaraj, G., Iyer, V.N. & Davies, R.W. 1988 Cloning of genes of the chitin utilization regulon of Serratia liquefaciens. Journal of Bacteriology 170, 2984–2988.

    Google Scholar 

  • Kang, M.S., Elango, N., Mattia, E., Au-Young, J., Robbins, P.W. & Cabib, E. 1984 Isolation of chitin synthetase from Saccharomyces cerevisiae. Purification of an enzyme by entrapment in reaction product. Journal of Biological Chemistry 259, 14966–14972.

    Google Scholar 

  • Kawasumi, T., Kiuchi, N., Futatsugi, Y., Ohba, K. & Yanagi, S.O. 1987 High yeild preparation of Lentinus edodes (“Shiitake”) protoplasts with regeneration capacity and mating type stability. Agricultural and Biological Chemistry 51, 1649–1656.

    Google Scholar 

  • Kelkar, H.S., Shankar, V. & Deshpande, M.V. 1990 Rapid isolation and regeneration of Sclerotium rolfsii protoplasts and their potential application for starch hydrolysis. Enzyme and Microbial Technology 12, 510–514.

    Google Scholar 

  • Kless, H., Sitrit, Y., Chet, I. & Oppenheim, A.B. 1989 Cloning of the gene coding for chitobiase of Serratia marcescens. Molecular and General Genetics 217, 471–473.

    Google Scholar 

  • Koga, D., Hirata, T., Sueshige, N., Tanaka, S. & Ide, A. 1992 Induction patterns of chitinases in Yam callus by inoculation with autoclaved Fusarium oxysporum, ethylene, & chitin & chitosan oligosaccharides. Bioscience Biotechnology and Biochemistry 56, 280–285.

    Google Scholar 

  • Koga, D., Sueshige, N., Orikono, K., Utsumi, T., Tanaka, S., Yamada, Y. & Ide, A. 1988 Efficiency of chitinolytic enzymes in the formation of Tricholoma matsutake protoplasts. Agricultural and Biological Chemistry 52, 2091–2093.

    Google Scholar 

  • Kuranda, M.J. & Robbins, P.W. 1991 Chitinase is required for cell separation during growth of Saccharomyces cerevisiae. Journal of Biological Chemistry 266, 19758–19767.

    Google Scholar 

  • Leah, R., Tommerup, H., Svendsen, I. & Mundy, J. 1991 Biochemical and molecular characterization of three barley seed proteins with antifungal properties. Journal of Biological Chemistry 266, 1564–1573.

    Google Scholar 

  • Lindsay, G.J.H. & Gooday, G.W. 1985 Action of chitinase in spines of the diatom Thalassiosira fluviatilis. Carbohydrate Polymers 5, 131–140.

    Google Scholar 

  • Mauch, F. & Staehelin, L.A. 1989 Functional implications of the subcellular localization of ethylene-induced chitinase & β-1,3 glucanase in bean leaves. Plant Cell 1, 447–457.

    Google Scholar 

  • Miyashita, K., Fujii, T. & Sawada, Y. 1991 Molecular cloning and characterization of chitinase gene from Streptomyces lividans 66. Journal of General Microbiology 137, 2065–2072.

    Google Scholar 

  • Murao, S., Kawada, T., Itoh, H., Oyama, H. & Shin, T. 1992 Purification and characterization of a novel type of chitinase from Vibrio alginolyticus TK-22. Bioscience Biotechnology and Biochemistry 56, 368–369.

    Google Scholar 

  • Muzzarelli, R.A. 1977 Chitin. New York: Pergamon Press.

    Google Scholar 

  • Nanjo, F., Ishikawa, M., Katsumi, R. & Sakai, K. 1990 Purification, properties, and transglycosylation reaction of β-N-Acetylhexosaminidase from Nocardia orientalis. Agricultural and Biological Chemistry 54, 899–906.

    Google Scholar 

  • Nanjo, F., Sakai, K., Ishikawa, M., Isobe, K. & Usui, T. 1989 Properties and transglycosylation reaction of a chitinase from Nocardia orientalis. Agricultural and Biological Chemistry 53, 2189–2195.

    Google Scholar 

  • Neuhaus, J.M., Ahl-Goy, P., Hinz, U., Flores, S. & Meins, F. 1991 High-level expression of a tobacco chitinase gene in Nicotiana sylvestris. Susceptibility of transgenic plants to Cercospora nicotianae infection. Plant Molecular Biology 16, 141–151.

    Google Scholar 

  • Nicol, S. 1991 Life after death for empty shells. New Scientist 129, 46–48.

    Google Scholar 

  • Ohtakara, A. 1988a Viscometric assay for chitinase. Methods in Enzymology 161, 426–430.

    Google Scholar 

  • Ohtakara, A. 1988b Chitosanase from Streptomyces griseus. Methods in Enzymology 161, 505–510.

    Google Scholar 

  • Ohtakara, A. & Mitsutomi, M. 1988 Analysis of chitooligosaccharides and reduced chitooligosaccharides by high-performance liquid chromatography. Methods in Enzymology 161, 453–457.

    Google Scholar 

  • Ordentlich, A., Elad, Y. & Chet, I. 1988 The role of chitinase of Serratia marcescens in biocontrol of Sclerotium rolfsii. Phytopathology 78, 84–88.

    Google Scholar 

  • peberdy, J.F. 1985 Mycolytic enzymes. In Fungal Protoplasts—Applications in Biochemistry and Genetics, eds Peberdy, J.F. & Ferenczy, L. pp. 31–44. New York: Marcel Dekker.

    Google Scholar 

  • Pe'er, S. & Chet, I. 1990 Trichoderma protoplast fusion: a tool for improving biocontrol agents. Canadian Journal of Microbiology 36, 6–9.

    Google Scholar 

  • Pelletier, A. & Sygusch, J. 1990 Purification and characterization of three chitosanase activities from Bacillus megaterium P1. Applied and Environmental Microbiology 56, 844–848.

    Google Scholar 

  • Reid, J.D. & Ogrydziak, D.M. 1981 Chitinase-overproducing mutant of Serratia marcescens. Applied and Environmental Microbiology 41, 664–669.

    Google Scholar 

  • Revah-Moiseev, S. & Carroad, P.A. 1981 Conversion of the enzymatic hydrolysate of shellfish waste chitin to single-cell protein. Biotechnology and Bioengineering 23, 1067–1078.

    Google Scholar 

  • Ridout, C.J., Coley-Smith, J.R. & Lynch, J.M. 1988 Fractionation of extracellular enzymes from a mycoparasitic strain of Trichoderma harzianum. Enzyme and Microbial Technology 10, 180–187.

    Google Scholar 

  • Robbins, P.W., Albright, C. & Benfield, B. 1988 Cloning and expression of a Streptomyces plicatus chitinase (chitinase-63) in Escherichia coli. Journal of Biological Chemistry 263, 443–447.

    Google Scholar 

  • Robbins, P.W., Overbye, K., Albright, C., Benfield, B. & Pero, J. 1992 Cloning and high-level expression of chitinase-encoding gene of Streptomyces plicatus. Gene 111, 69–76.

    Google Scholar 

  • Roberts, W.K. & Selitrennikoff, C.P. 1988 Plant and bacterial chitinases differ in antifungal activity. Journal of General Microbiology 134, 169–176.

    Google Scholar 

  • Sakai, K., Uchiyama, T., Matahira, Y. & Nanjo, F. 1991 Immobilization of chitinolytic enzymes and continuous production of N-acetylglucosamine with the immobilized enzymes. Journal of Fermentation and Bioengineering 72, 168–172.

    Google Scholar 

  • Schlumbaum, A., Mauch, F., Vogeli, U. & Boller, T. 1986 Plant chitinases are potent inhibitors of fungal growth. Nature 324, 365–367.

    Google Scholar 

  • Shapiro, R., Ordentlich, A., Chet, I. & Oppenheim, A.B. 1989 Control of plant diseases by chitinase expressed from cloned DNA in Escherichia coli. Phytopathology 79, 1246–1249.

    Google Scholar 

  • Shinshi, H., Neuhaus, J.M., Ryals, J. & Meins, F.J. 1990 Structure of a tobacco endochitinase gene: evidence that different chitinase genes can arise by transposition of sequences encoding a cysteine-rich domain. Plant Molecular Biology 14, 357–368.

    Google Scholar 

  • Smirnoff, W.A. 1975 Insecticidal compositions. US Patent 3,911,110 (CA 84, P39727b).

  • St. Leger, R.J., Cooper, R.M. & Charnley, A.K. 1986 Cuticledegrading enzymes of entomopathogenic fungi: cuticle degradation in vitro by enzymes from entomopathogens. Journal of Invertebrate Pathology 47, 167–177.

    Google Scholar 

  • Sundheim, L., Poplawsky, A.R. & Ellingboe, A.H. 1988 Molecular cloning of two chitinase genes from Serratia marcescens and their expression in Pseudomonas species. Physiological and Molecular Plant Pathology 33, 483–491.

    Google Scholar 

  • Tanaka, H., Ogasawara, N., Nakajima, T. & Tamari, K. 1970 Cell walls of Piricularia oryzae. I. Selective enzymolysis of Piricularia oryzae walls by wall-lytic enzymes of Bacillus circulans WL 12. Journal of General and Applied Microbiology 16, 39–60.

    Google Scholar 

  • Thakur, M.S., Kennedy, M.J. & Karanth, N.G. 1991 An environmental assessment of biotechnological processes. Advances in Applied Microbiology 36, 67–86.

    Google Scholar 

  • Uchida, Y. & Ohtakara, A. 1988 Chitosanase from Bacillus species. Methods in Enzymology 161, 501–505.

    Google Scholar 

  • Ueno, H., Miyashita, K., Sawada, Y. & Oba, Y. 1990 Purification and some properties of extracellular chitinases from Streptomyces species S-84. Journal of General and Applied Microbiology 36, 377–392.

    Google Scholar 

  • Usui, T., Matsui, H. & Isobe, K. 1990 Enzymic synthesis of chitooligosaccharides utilizing transglycosylation by chitinolytic enzymes in a buffer containing ammonium sulfate. Carbohydrate Research 203, 65–77.

    Google Scholar 

  • Verburg, J.G. & Huynh, K. 1991 Purification and characterization of an antifungal chitinase from Arabidopsis thaliana. Plant Physiology 95, 450–455.

    Google Scholar 

  • Verburg, J.G., Smith, C.E., Lisek, C.A. & Huynh, Q.K. 1992 Identification of an essential tyrosine residue in the catalytic site of a chitinase isolated from Zea mays that is selectively modified during inactivation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. Journal of Biological Chemistry 267, 3886–3893.

    Google Scholar 

  • Vyas, P.R. & Deshpande, M.V. 1989 Chitinase production by Myrothecium verrucaria and its significance for fungal mycelia degradation. Journal of General and Applied Microbiology 35, 343–350.

    Google Scholar 

  • Vyas, P.R. & Deshpande, M.V. 1991 Enzymatic hydrolysis of chitin by Myrothecium verrucaria chitinase complex and its utilization to produce SCP. Journal of General and Applied Microbiology 37, 267–275.

    Google Scholar 

  • Wortman, A.T., Somerville, C.C. & Colwell, R.R. 1986 Chitinase determinants of Vibrio vulnificus: gene cloning and applications of a chitinase probe. Applied and Environmental Microbiology 52, 142–145.

    Google Scholar 

  • Yanagi, S.O. & Takebe, I. 1984 An efficient method for isolation of mycelial protoplasts from Coprinus macrorhizus and other basidiomycetes. Applied Microbiology and Biotechnology 19, 58–60.

    Google Scholar 

  • Zikakis, J.P. 1989 Chitinolytic enzymes and their application. In Biocatalysts in Agricultural Biotechnology, ACS Symposium No. 389, eds Whitaker, J.R. & Sonnet, P.E. pp. 116–126. Washington DC: American Chemical Society.

    Google Scholar 

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  1. Biochemical Sciences Division, National Chemical Laboratory, Pune-411008, India

    S. A. Shaikh & M. V. Deshpande

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Shaikh, S.A., Deshpande, M.V. Chitinolytic enzymes: their contribution to basic and applied research. World Journal of Microbiology and Biotechnology 9, 468–475 (1993). https://doi.org/10.1007/BF00328035

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